不同浓度苦丁茶对TCA8113人舌鳞癌细胞的体外抗癌效果
Anticancer effect of different concentrations of Ilex kudingcha C.J.Tseng in TCA8113 human tongue squamous carcinoma cells in vitro
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摘要: 对一种市售苦丁茶进行TCA8113人舌鳞癌细胞体外抗癌效果评价。通过MTT（3-（4,5-二甲基噻唑-2）-2,5-二苯基四氮唑溴盐）比色法实验、DAPI（4’,6-二脒基-2-苯基吲哚）荧光染色分析、RT-PCR（逆转录聚合酶链式反应）和Westernblot分析,研究其抗癌效果。通过MTT法测定苦丁茶对癌细胞体外生长抑制效果时发现,200μg/mL浓度的苦丁茶（75%癌细胞生长抑制率）对TCA8113人舌鳞癌细胞生长有相对最强的抑制效果。DAPI染色分析显示,与100、50μg/mL苦丁茶相比,使用200μg/mL浓度的苦丁茶,对TCA8113细胞有较强的诱其凋亡的能力。在RT-PCR和Westernblot分析结果中,凋亡因子Bax（B细胞淋巴瘤/白血病基因伴随蛋白x）、caspase-3（半胱天冬氨酸酶-3）、caspase-9（半胱天冬氨酸酶-9）的mRNA和蛋白质表达得到增强,Bcl-2（B细胞淋巴瘤/白血病基因-2）表达减弱。综合分析得出,一定浓度的苦丁茶具有良好的防癌抗癌效果。Abstract: One Ilex kudingcha C.J.Tseng product was sold for the evaluation of in vitro anticancer effect in TCA8113 human tongue squamous carcinoma cells.In MTT assay, apoptosis analysis by DAPI staining and RT-PCR, Western blot gene expressions were employed to check their anticancer effect.Using MTT assay to check the in vitro cancer cell growth inhibitory effect, at the concentration of 200μg/mL, Ilex kudingcha C.J.Tseng (75% inhibition rates) showed the best inhibitory effect on the growth of TCA8113 human tongue squamous carcinoma cells.DAPI staining showed that 200μg/mL of Ilex kudingcha C.J.Tseng induced apoptosis on TCA8113 cells.Ilex kudingcha C.J.Tseng (200μg/mL) significantly induced apoptosis, up regulated mRNA and protein expressions of Bax, caspase-3 and caspase-9, and down-regulated Bcl-2 expression, compared with 100 and 50μg/mL of Ilex kudingcha C.J.Tseng.These results suggested that Ilex kudingcha C.J.Tseng showed good cancer preventive effect.
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