动物组织中呋喃妥因代谢物残留酶联免疫试剂盒的研制
Development of an enzyme linked immunosorbent assay kit for furantoin metabolite residues determination in animal tissue
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摘要: 通过对呋喃妥因代谢物分子结构进行改造,制备了半抗原及人工抗原,免疫动物,制备特异性强的单克隆抗体。在筛选呋喃妥因代谢物单克隆抗体的基础上,建立酶联免疫检测方法,并研制出检测动物组织中呋喃妥因代谢物残留的试剂盒。该试剂盒的IC50值为0.094μg/L,最低检测限为0.1μg/kg,样本添加回收率为78.2%～102.7%,变异系数为5.1%～11.4%;与呋喃妥因的交叉反应率为13%,与其他药物的交叉反应率均小于1%;对实际样本的检测结果与液相色谱-串联质谱法基本一致。该方法快速、灵敏、可靠,为动物组织中呋喃妥因代谢物的残留检测提供了便捷、准确的分析检测手段。Abstract: Artificial hapten and immunogen were synthesized, after which monoclonal antibodies against furantoin metabolite was raised.On the basis of furantoin metabolite monoclonal antibody, rapid test enzyme linked immunosorbent assay (ELISA) kit for furantoin metabolite residues in animal tissue was developed.The sensitivity and detection limit of ELISA kit were 0.05μg/L and 0.1μg/kg, respectively.Sample recoveries were 78.2%～102.7%, and the coefficients of variation were 5.1%～11.4%.The cross-reactivity towards furantoin was 13%.As for other drugs, the cross-reactivity was below 1%.The results of ELISA kit and liquid chromatographic tandem mass spectrometry (LC-MS/MS) were in accordance.This method was rapid, sensitive and reliable, which provided a convenient and accurate way for detection of furantoin metabolite residues.
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