Abstract: Effects of preharvest spraying of antagonistic yeast Cryptococcus laurentii on the decay and quality of post harvest strawberry (Toyonoka) under 20℃conditions for 4d were investigated.The result showed that the concentration of 10 8 CFU/mL of yeast cell suspension spraying could effectively reduce strawberry fruit rot caused by Botrytis cinerea as well as natural disease, the more the number of preharvest spraying, the better the inhibitory efficacy.The decay index of fruits sprayed with antagonistic yeast three times was reduced 68.31% compared with the control after storage.Antagonistic yeast before harvest spraying could reduce the weight loss rate, delay the hardness and V C decrease of strawberry fruit, but had no significant effect on contents of soluble solids, titratable acidity and fruit surface color.Bengal agar plate test results showed that antagonistic yeast colonization could be found in the spraying treated fruit epidermis.