Abstract: Fishmeal which was produced by wet process that was used as the raw material. Organic solvent extraction for fish oil from fishmeal was optimized. Processing parameters of extraction including extract solvent, extract time and extract temperature were investigated on quality indicators (yield) of fish oil by single factor experiments. Optimum operating parameters were obtained by response surface methodology. Best conditions to produce fish oil were petroleum ether, extract time 3.5h and extract temperature 64℃ . The predicted results of yield of fish oil 19.5%±0.028% were close to the experimental values 19.4% ±0.874% , the content of protein in fishmeal had been improved from 55.9%±0.534% to 68.0%±0.251% , fulfill requirement of the national standard for super fishmeal.