牛鼻栓三裂轮簇霉菌发酵产物的抗氧化活性研究

Antioxidant activity of fermentation broth extracts of Verticicladium trifidum from Fortunearia sinensis

摘要: 为了探讨筛选出的具有较高总抗氧化活性的牛鼻栓三裂轮簇霉菌（Verticicladiumtrifidum）发酵液提取物,本研究制备了菌株发酵液粗提取物,比较了其抗脂质过氧化作用和清除超氧阴离子自由基（O-2.）、羟自由基（.OH）、二苯基苦基苯肼自由基（DPPH.）的能力。结果表明:该菌株发酵液提取物的抗氧化活性不仅高于阳性对照芦丁,而且具有良好的稳定性。浓度为1.0mg/mL时,对.OH具有一定的清除作用,但弱于芦丁,清除率为74.12%;对O-2.和DPPH.均具有较强的清除作用,清除能力优于芦丁,清除率分别达到84.25%和84.27%。

Abstract: The antioxidant capacity of an endophytic Verticicladium trifidum No.48 isolated from Fortunearia sinensis was studied.The reducing power (total antioxidant capacity) and radical scavenging abilities of its fermentation broth extracts were compared with rutin.The results showed that the extracts of the isolate had stronger antioxidant activity with good stability than rutin.According to the evaluation by O-2·, ·OH, DPPH· scavenging in present study, O-2· and DPPH· scavenging activities of the extracts reached 84.25% and 84.27% at the concentration of 1.0mg/mL, respectively.On the other hand, the scavenging rate against ·OH was 74.12% at the concentration of 1.0mg/mL.