高效液相色谱法测定浓缩果汁及水果果糖中5-羟甲基糠醛

Determination of 5-hydroxymethylfurfural in fruit juice concentrate and fruit fructose by HPLC

摘要: 建立了浓缩苹果汁、浓缩梨汁和水果果糖中的5-羟甲基糠醛的高效液相色谱（HPLC）检测方法。样品用甲醇溶解后,经水稀释,InetrsilODS-3C18（250mm×4.6mm,5μm）色谱柱分离,紫外检测器在282nm处进行检测;5-羟甲基糠醛在1.0～25.0mg/L范围内线性关系良好,相关系数为0.9998,回收率为82.2%～103.3%,精密度（RSD）为0.62%～1.25%,方法的检出限（LOD）为0.2mg/kg。本方法具有快速、简单、灵敏度高、适用范围广等特点,可以满足果汁中5-羟甲基糠醛的分析要求。

Abstract: A HPLC method was established for the determination of 5-hydroxymethylfurfural (5-HMF) in fruit juice concentrate and fruit fructose. The sample was diluted with water, separated by using Inetrsil ODS-3 Cl8 (250mm×4. 6mm, 5μm) chromatographic column. The method showed that a good linearity within the range of 1. 0 ~ 25. 0mg/L (r=9998) . The LOD of 5-HMF was 0. 2mg/kg, the average recoveries from 82. 2% to 103. 3%, the relative standard deviation (RSD) from 0. 62% to 1. 25%. The HPLC method is quick, accurate, sensitive and suitable for determining the content of 5-HMF in fruit juice concentrate.