田口设计优化耶氏酵母生产γ-癸内酯

Optimization of culture medium for γ-decalactone production by Yarrowia sp.using Taguchi design

摘要: 通过田口设计对产γ-癸内酯的培养基成分进行优化。根据单因素实验结果,利用Minitab软件设计正交实验,采用田口设计方法分析正交实验中各因素水平的均值情况和信噪比情况。实验结果表明,复合氮源即酵母膏和（NH4）2HPO4对γ-癸内酯产量影响极显著,MgSO.47H2O对γ-癸内酯产量影响显著。软件预测培养基的最优组合为麸皮20g/L、酵母膏7.5g/L、（NH4）2HPO416.5g/L、KH2PO46g/L、MgSO4.7H2O0.5g/L,预测产量为2.49g/L,信噪比为9.04dB,经验证γ-癸内酯产量为2.65g/L,信噪比为9.68dB,优化方案达到了预期效果,具有较好的稳定性。

Abstract: Culture medium for γ-decalacton production by Yarrowia sp.was optimized by using Taguchi design.First, single factor was tested, then orthogonal experiment was designed to carry out using Minitab software, and the result of the mean and SNR of level of each factor were analyzed by using Taguchi design.The result proved that the effect of composite nitrogen was very significant, and (NH4) 2HPO4 was significant.Software predicted the optimal culture medium consisted of 20g/L wheat bran, 7.5g/L yeast extract, 16.5g/L (NH4) 2HPO4, 6g/L KH2PO4 and 0.5g/L MgSO4·7H2O, and GDL yield was 2.49g/L, the SNR was 9.04dB.The γ-decalacton yield of verification experiment was 2.65g/L, and the SNR was 9.68dB.The result showed that the optimal solution achieved the desired effect with good stability.