Abstract: Objective:Study the preservation methods about the CHE, in order to get the best enzyme activity.Methods:Based wheat on the testing material, took cold storage, frozen and enzyme immobilization measures, adding different types and different proportions of preservatives, using the different packing material were investigated.Results:The result showed that the better effect of remain well enzyme solution of the enzyme activity was set for-20℃ temperature preservation, adding 0.1% thimerosal used plastic bottles packaging storage.Conclusion:The cold preservation CHE and adding regular doses of preservative could make cholinesterase enzyme very good maintenance.