Abstract: 17 isolates of lactic acid bacteria (LAB) were isolated from the traditional fermented milk gathering from the herdmen's home in Hulunbeier of Inner Mongolia by traditional pure culture method.Based on 16S rDNA sequence analysis, pheS sequence analysis and physiological and biochemical test, these isolates were identified as 11 strains of Lactococcus lactic.subsp.lactic, 1 strain of Lactococcus garvieae, 1 strain of Enterococcus faecalis, two strains of Lactobacillus plantarum subsp.plantarum and 2 strains Lactobacillus curvatus.Lactococcus lactic.subsp.lactic was the predominant strain (64.7% of the total isolates) .