Abstract: The blackberry seed oil was extracted by soxhlet methods from blackberry seed, and the effect of different solvents, grinding degree, extraction temperature, extraction time, solid-liquid ratio on the oil yield extraction were investigated.The optimal conditions were determined by orthogonal test as follows:benzine as solvents, grinding degree 80 mu, extraction temperature 90℃, extraction time 2h, solid-liquid ratio 1∶15 (g∶mL) .Under the optimal conditions, the yield of blackberry seed oil was 22.855%.