Abstract: Aqueous enzymatic extraction process with extrusion pretreatment was used in this experiment to extract blending oil and the response surface method was employed to optimize the parameters in the experiment. The optimum parameters were as follows:enzyme additive amount for 1.84%, hydrolysis temperature for 50℃, hydrolysis time for 3.3h, solids-to-liquid ratio for 17.6%, pH 8. Proven experiment showed that extraction rate of blending oil could reach 91.67%. Blending oil which was extracted met the demands that the ratio of saturated fatty acids, monounsaturated fatty acids and polyunsaturated fatty acids equals to 0.46∶1∶1, and that the ratio of n-6 polyunsaturated fatty acids and n-3 polyunsaturated fatty acids equals to 4∶1.