Abstract: The effectiveness of a single treatment with natamycin, tea polyphenol and phytic acid in the preservation of bundle of hoof of Gaogou stored at 37℃ was investigated by examining the change of total bacterial count at intervals of 10d.In order to obtain the optimal preservation for bundle of hoof of Gaogou, the combined use of the nature preservatives was adopted and their optimum addition proportions were explored for maximizing weighed score of sensory evaluation and total bacterial count of bundle of hoof of Gaogou using orthogonal array design of dual indicators.The result showed that each natural preservative could significantly inhibit microbiological contamination and natamycin exhibited the highest preservation effect among them.The optimal formula of compound preservatives consisted of natamycin 0.06%, tea polyphenol 0.10%, phytic acid 0.04%.This formula had much stronger inhibitory effect against bacteria than a single preservative.