Abstract: Using lomefloxacin as hapten, the immunogen was prepared with water soluble carbodiimide (EDC) method, and the coating agent was synthesized with active ester method.The polyclonal antibody was obtained successfully after immunization.Characterized by indirect competitive enzyme linked immunosorbent assay (icELISA) , the antibody exhibited strong broad-specificity, 11 fluoroquinolone drugs could be recognized with a limit of detection lower than 10ng/mL, which could meet the requirement of related international organization.This study would benefit to the immunoassay development for the simultaneous detection of many fluoroquinolone drugs.