Abstract: In this research, a strain of trichoderma and aspergillus filtered out from the environment was used to ferment in powder-liquid medium where the main carbon source was rice straw.Along with the fermentation, the pretreated rice straw powder was catalytic degraded into glucose by enzyme.There was orthogonal test based on single factor experiment.The orthogonal experiment showed that the crude enzyme liquid dosage caused the biggest influence on the degradation of straw powder, second was temperature and followed by pH value, and the straw powder concentration caused the minimum effects on degradation.The optimal conditions of degradation of rice straw powder was:the percentage of the volume of crude enzyme liquid in total degradation liquid was 3%, initial straw powder concentration was 60g/L, the pH value was 4.8, the degradation temperature was 50℃.Under the optimal conditions, the degradation rate could be able to reach 80%.