响应面法优化沙苑子中黄酮类化合物超声辅助提取工艺
详细信息Ultrasound-assisted extraction technology for flavones from Semen Astragali Complanati optimized by response surface methodology
-
摘要: 为了获得沙苑子黄酮类化合物的最佳提取工艺参数,选取液料比、超声温度、沙苑子粒度、超声时间作为实验因素,以提取物的吸光度为响应值,采用响应面实验设计法对工艺参数进行优化。结果表明:沙苑子黄酮类丙酮提取物在323nm处有最大吸光值;60%丙酮为最佳提取剂;最佳工艺参数为粒度120目,超声温度50℃,超声时间60min,液料比40:1(mL/g),粗提率为16.19%。环境扫描电镜显示,超声处理后沙苑子粉的表面出现了蜂窝状形貌图,从而促使胞内物质溶出,提高了提取率。Abstract: In order to obtain the optimum extraction conditions for flavoness from Semen Astragali Complanati, on the basis of one-factor experiment, the ratio of solvent-to-sample, ultrasonic extraction temperature, particle size and ultrasonic extraction time were optimized by response surface methodology (RSM) and the absorption of extracts for flavones was chosen as the response.The results showed as follows:the maximum absorbance wavelength for the flavones extracts was 323nm, the suitable extractant was 60% acetone, the particle size was 120 mesh, the ultrasonic extraction temperature was 50℃, the ultrasonic extraction time was 60min, and the ratio of solvent-to-sample was 40:1 (mL/g) .Under these conditions, the crude yield was 16.19%.The morphology for the particles was studied by scanned electron microscopy, many honey-combed microstructures appeared, and hence facilitated the mass transfer and promoted extraction efficiency.
-
[1] 汤臣康, 谢人明.沙苑子和黄芪药理作用研究Ⅰ-对麻醉动物脑血流量的影响[J].西北药学杂志, 1987, 2 (1) :5-8. [2] 刘春宇, 顾振纶, 韩蓉, 等.沙苑子提取物对小鼠四氯化碳肝损伤的保护作用[J].中草药, 2002, 33 (12) :1104-1106. [3] 刘春宇, 顾振纶, 韩蓉, 等.沙苑子黄酮对CCl4及D-氨基半乳糖致急性肝损伤的保护作用[J].中草药, 2005, 36 (12) :1838-1841. [4] 张清安, 范学辉, 张志琪, 等.沙苑子提取物中多酚含量的测定[J].食品科学, 2010, 31 (14) :178-181. [5] 常玉华, 张清安.沙苑子化学成分研究现状与展望[J].陕西农业科学, 2011 (6) :129-131, 186. [6] 庞来祥, 刘燕, 刘小民, 等.沙苑子中总黄酮的提取工艺研究[J].西北药学杂志, 2008, 23 (4) :214-216. [7] 李君玲, 王大伟.正交设计优选沙苑子总黄酮的提取工艺[J].中华中医药学刊, 2008, 26 (7) :1588-1589. [8] 于猛, 竺梅, 王英锋, 等.沙苑子中沙苑子苷A超声波提取工艺及含量测定[J].首都师范大学学报:自然科学版, 2011, 32 (2) :41-44. [9] 刘银芳, 吴文倩, 刘春宇, 等.沙苑子黄酮提取工艺研究[J].时珍国医国药, 2010, 21 (6) :1401-1402. [10] 李洪娟, 孙居锋, 代现平.沙苑子总黄酮的超声波提取工艺[J].湖北农业科学, 2009, 48 (6) :1479-1480. [11] Qing-An Zhang, Zhi-Qi Zhang, Xuan-Feng Yue, et al.Response surface optimization of ultrasound-assisted oil extraction from autoclaved Almond powder[J].Food Chemistry, 2009, 116:513-518.
[12] Qing-An Zhang, Xue-Hui Fan, Zhi-Qi Zhang, et al.Optimization of SC-CO2extraction of oil from almond pretreated with autoclaving[J].LWT-Food Science and Technology, 2009, 42:1530-1537.
计量
- 文章访问数:
- HTML全文浏览量:
- PDF下载量:
下载:
