Abstract: The extruding experiment by two-screws extruder was studied with the soy flake as materias.The high denatured soybean meal was obtained from soybean flakes by extrusion, degreasing and desolventizing at high temperature.The effect of moisture content of materials, barrel temperature, the rotating speed of screws and die diameter on the peptide yield of enzymatic high denatured soybean meal were studied.Single factor experiments and orthogonal test were applied to analyze the factors.The contribution rate of factor was as follow:moisture content of materials 20%, barrel temperature 60℃, the rotating speed of screws 100r/min and die diameter 15mm, the peptide yield can reached at 36.88%.Extrusion can effectively improve the utilization of soybean meal to provide a theoretical basis for future industrial production.