Abstract: The ethanol deposition technology of chondroitin sulfate was studied in the paper, witch was extracted from bovine nose cartilage hydrolyzed by alkaline solution. The optimal ethanol deposition conditions of chondroitin sulfate were explored by single factor and orthogonal experiments to be ethanol solution of 65%, sodium acetate solution of 4.5%, pH6.0. Under the optimal conditions, the yield was 14.81%, the content of glucuronic acid was 33.36%, the purity quotient of chondroitin sulfate was 85.9%.UV analysis revealed that the absorption peak of chondroitin sulfate appears at 196nm, while characteristic absorption peaks of protein (280nm) and nucleic acid (260nm) were not found.