Optimization of Ultrahigh Pressure Assisted Micellar Extraction of Taxifolin from Larch

In this study, in order to simplify the extraction process of taxifolin, cut costs and energy sources, improve extraction efficiency, and promote comprehensive application of taxifolin. Taxifolin was extracted from the trunk of Larix gemlini by ultra-high pressure assisted micellar green solvent extraction technology, using Larix gemlini of Heilongjiang Province as raw material. Based on the total content of taxifolin in different parts of larch root and trunk, tea saponin was selected as the most appropriate surfactant from candidate surfactants. The extraction process was optimized by response surface experiment, investigating effects of solid-liquid ratio, extraction pressure, extraction times and micellar concentration on the extraction rate of taxifolin. Results showed that, the optimal extraction process conditions were selected as follows: The tea saponin concentration was 8%, the solid-liquid ratio was 1:11.5, the extraction pressure was 157 MPa, the extraction times were 3 times, the holding time was 5 min. The experiment was repeated for 3 times under the optimum condition, and the actual extraction rate of taxifolin was 84.35%±1.2%, which was basically consistent with the predicted value of 84.98%. Compared with different extraction processes such as microwave extraction, ultrasonic extraction and reflux extraction, the energy consumption and CO₂ emission per unit of raw material of ultra-high pressure assisted micelle extraction were the lowest, which was 1.64×10⁻⁴ kW·h·g⁻¹ and 1.29×10⁻⁴ kg/g respectively. To summarize, the extraction of taxifolin from larch by ultra-high pressure assisted micellar green solvent extraction technology could be used widely which was environmentally friendly, simple, stable, reasonable and reliable.