Effects of Growth Period and Germination Treatment on Saponin Content of Different Bean Varieties

GUAN Tingting; KOU Yu; ZHANG Yiting; LIU Dajun; SONG Yong; SUN Qingshen

doi:10.13386/j.issn1002-0306.2022120108

Volume 44 Issue 20

Oct. 2023

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Science and Technology of Food Industry > 2023 > 44(20): 70-76. > DOI: 10.13386/j.issn1002-0306.2022120108

GUAN Tingting, KOU Yu, ZHANG Yiting, et al. Effects of Growth Period and Germination Treatment on Saponin Content of Different Bean Varieties[J]. Science and Technology of Food Industry, 2023, 44(20): 70−76. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022120108.

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Effects of Growth Period and Germination Treatment on Saponin Content of Different Bean Varieties

1.
School of Life Sciences, Heilongjiang University, Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang Provincial Key Laboratory of Ecological Restoration and Resource Utilization for Cold Region, Key Laboratory of Microbiology, College of Heilongjiang Province, Harbin 150080, China
2.
Hebei University of Environmental Engineering, Hebei Key Laboratory of Agroecological Safety,Qinhuangdao 066102, China
3.
College of Advanced Agricultural, Ecological and Environmental Sciences,Heilongjiang University, Harbin 150080, China

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Received Date: December 13, 2022
Available Online: August 20, 2023

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Abstract

Abstract

In order to improve the processing and utilization value of the Northeast snap beans products by clarifying the saponin content, sites and influencing factors, this study extracted saponins from the seeds and pods of seven kinds of Northeast snap beans, including Golden Crown, Green Crown, and Sheng Crown within 10~40 d after blooming using ultrasonic-assisted methods. 0.4%~1.2% sodium chloride solution was used to conduct germination treatment under salt stress on the saponin content of Golden Crown and Mantangcai seed. Finally, ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS) was used to identify the saponin species in the seeds of Golden Crown. The results showed that saponin existed mainly in the seeds, and the saponin content increased firstly but then decreased with the increase of growth time. The peak period of saponin content varied with the bean varieties. Compared with the other six varieties of snap beans, the saponin content in the full hall was the highest, with the highest saponin content of Mantangcai whole pods of 23.62±0.14 mg/g at 35 d. The change of saponin content was closely related to the sprouting time of bean seeds and bean varieties. Salt stress treatment could significantly increase the saponin content of Mantangcai seeds from (7.08±0.15) mg/g to (10.15±0.55) mg/g. The UHPLC-Q-TOF-MS analysis showed that there were seven saponins in the crude saponins of the Golden Crown bean, including saikosaponin F, adzuki saponin II and astragaloside IV. This study provides data support for the in-depth development of soybean saponins in Northeast China.
- snap bean saponins,
- different growth periods,
- germination treatment,
- component analysis

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References (31)

References

[1]	李香梅, 侯志敏, 肖宇, 等. 豆角豆荚和种子中皂苷含量的比较[J]. 安徽农业科学,2009,37(17):7944−7946 doi: 10.3969/j.issn.0517-6611.2009.17.056 LI X M, HOU Z M, XIAO Y, et al. Comparison of saponin content in bean pods and seeds[J]. Anhui Agricultural Sciences,2009,37(17):7944−7946. doi: 10.3969/j.issn.0517-6611.2009.17.056
[2]	李健, 金龙哲, 刘宁, 等. 豆角总皂苷提取工艺的研究[J]. 食品与发酵工业,2007(1):127−130 doi: 10.3321/j.issn:0253-990X.2007.01.033 LI J, JIN L Z, LIU N, et al. Study on extraction technology of total saponins from beans[J]. Food and Fermentation Industry,2007(1):127−130. doi: 10.3321/j.issn:0253-990X.2007.01.033
[3]	陈莉, 吴耀生. 三萜皂苷生物合成途径及相关酶[J]. 国外医药(植物药分册),2004(4):156−161 CHEN L, WU Y S. Biosynthesis pathway of triterpenoid saponins and related enzymes[J]. Foreign Medicine (Botanical Medicine),2004(4):156−161.
[4]	徐圆圆, 陈仲, 贾黎明, 等. 植物三萜皂苷生物合成途径及调控机制研究进展[J]. 中国科学:生命科学,2021,51(5):525−555 XU Y Y, CHEN Z, JIA L M, et al. Research progress on biosynthesis pathway and regulation mechanism of plant triterpenoid saponins[J]. China Science:Life Sciences,2021,51(5):525−555.
[5]	GUO S Y, YIN Y, LEI T, et al. A cycloartenol synthase from the steroidal saponin biosynthesis pathway of paris polyphylla[J]. Journal of Asian Natural Products Research, 2021, 23(4): 353-362.
[6]	唐瑶, 陈洋, 曹婉鑫, 等. 三萜皂苷结构及应用的研究进展[J]. 安徽化工,2015,41(2):13−15,22 doi: 10.3969/j.issn.1008-553X.2015.02.005 TANG Y, CHEN Y, CAO W X, et al. Research progress on the structure and application of triterpenoid saponin[J]. Anhui Chemical Industry,2015,41(2):13−15,22. doi: 10.3969/j.issn.1008-553X.2015.02.005
[7]	刘宁, 李健, 金龙哲, 等. 超声波法提取豆角总皂苷的工艺研究[J]. 食品科学,2008(10):327−329 doi: 10.3321/j.issn:1002-6630.2008.10.074 LIU N, LI J, JIN L Z, et al. Study on ultrasonic extraction of total saponins from beans[J]. Food Science,2008(10):327−329. doi: 10.3321/j.issn:1002-6630.2008.10.074
[8]	隆逸瑶, 刘渝港, 夏会平, 等. 三萜皂苷抗肥胖及其作用机制的研究进展[J]. 食品工业科技,2022,43(7):445−456 LONG Y Y, LIU Y G, XIA H P, et al. Research progress of triterpenoid saponins in anti-obesity and its mechanism[J]. Food Industry Science and Technology,2022,43(7):445−456.
[9]	任师超, 孙秋艳, 冯旭东, 等. 微生物细胞工厂合成五环三萜皂苷类化合物[J]. 合成生物学,2022,3(1):168−183 doi: 10.12211/2096-8280.2020-082 REN S C, SUN Q Y, FENG X D, et al. Synthesis of pentacyclic triterpenoid saponins in microbial cell factory[J]. Synthetic Biology,2022,3(1):168−183. doi: 10.12211/2096-8280.2020-082
[10]	张慧颖, 肖旺钏, 郑可利, 等. 超声波法夏枯草总皂苷提取工艺研究[J]. 三明学院学报,2017,34(6):50−56 doi: 10.14098/j.cn35-1288/z.2017.06.008 ZHANG H Y, XIAO W C, ZHENG K L, et al. Ultrasonic assisted extraction of total saponins from Prunella vulgaris[J]. Journal of Sanming University,2017,34(6):50−56. doi: 10.14098/j.cn35-1288/z.2017.06.008
[11]	赵露, 苗玉华, 吴儒琳, 等. 不同盐胁迫对苦豆子种子萌发及幼苗生长的影响[J]. 山西农业科学, 2021, 49（7）: 844−848. doi: 10.3969/j.issn.1002-2481.2021.07.10 ZHAO L, MIAO Y H, WU R L, et al. Effects of different salt stress on seed germination and seedling growth of Sophora alopecuroides L.[J]. Shanxi Agricultural Sciences, 2021, 49（7）: 844−848. doi: 10.3969/j.issn.1002-2481.2021.07.10
[12]	侯亚楠, 王思尧, 魏朝俊, 等. 小豆皂苷提取和液质联用检测方法的优化[J]. 北京农学院学报,2020,35(3):17−21 doi: 10.13473/j.cnki.issn.1002-3186.2020.0304 HOU Y N, WANG S Y, WEI Z J, et al. Optimization of extraction and LC-MS detection methods of adzuki bean saponin[J]. Journal of Beijing Agricultural College,2020,35(3):17−21. doi: 10.13473/j.cnki.issn.1002-3186.2020.0304
[13]	李启照. 女贞不同部位中总皂苷的含量测定及提取工艺[J]. 怀化学院学报,2016,35(11):1−5 doi: 10.3969/j.issn.1671-9743.2016.11.001 LI Q Z. Determination and extraction technology of total saponins in different parts of Ligustrum lucidum[J]. Journal of Huaihua University,2016,35(11):1−5. doi: 10.3969/j.issn.1671-9743.2016.11.001
[14]	徐伟, 肖志强, 李纯懿, 等. 不同生长时间对美国皂荚壳皂苷的影响比较研究[J]. 林产化学与工业,2021,41(3):19−25 doi: 10.3969/j.issn.0253-2417.2021.03.003 XU W, XIAO Z Q, LI C Y, et al. Comparative study on properties of Gleditsia triacanthos L. shell saponins at different growth time[J]. Forest Products Chemistry and Industry, 2021, 41(3):19−25. doi: 10.3969/j.issn.0253-2417.2021.03.003
[15]	HUANG G C, CAI W X, XU B J. Improvement in beta-carotene, vitamin B2, gaba, free amino acids and isoflavones in yellow and black soybeans upon germination[J]. LWT-Food Science and Technology,2017,75:488−496. doi: 10.1016/j.lwt.2016.09.029
[16]	蔡晓锋, 胡体旭, 叶杰, 等. 植物盐胁迫抗性的分子机制研究进展[J]. 华中农业大学学报,2015,34(3):134−141 doi: 10.13300/j.cnki.hnlkxb.2015.03.039 CAI X F, HU T X, YE J, et al. Research progress on molecular mechanism of salt stress resistance in plants[J]. Journal of Huazhong Agricultural University,2015,34(3):134−141. doi: 10.13300/j.cnki.hnlkxb.2015.03.039
[17]	崔悦. 盐胁迫对人参转录因子及皂苷合成酶基因表达能力的影响[D]. 吉林:吉林大学, 2021 CUI Y. Effects of salt stress on gene expression ability of ginseng transcription factor and saponin synthase[D]. Jilin:Jilin University, 2021.
[18]	高春霞, 王凤忠, 袁莉, 等. 发芽过程中大豆活性物质、抗营养因子及抗氧化活性变化的综述[J]. 核农学报,2019,33(5):962−968 doi: 10.11869/j.issn.100-8551.2019.05.0962 GAO C X, WANG F Z, YUAN L, et al. Review on the changes of active substances, anti-nutritional factors and antioxidant activities of soybean during germination[J]. Journal of Nuclear Agriculture,2019,33(5):962−968. doi: 10.11869/j.issn.100-8551.2019.05.0962
[19]	AlAM M A, JURAIMI A S, RAFII M Y, et al. Effects of salinity and salinity-induced augmented bioactive compounds in purslane (Portulaca oleracea L.) for possible economical use[J]. Food Chemistry, 2015, 169(15):439−447.
[20]	SARKAR D, CHRISTOPHER A, SHETTY K. Phenolic bioactives from plant-based foods for glycemic control[J]. Frontiers in Endocrinology,2022,12:1921.
[21]	高晓娟, 郎多勇, 周丽, 等. NaCl胁迫对银柴胡药材活性成分含量及其积累量的影响研究[J]. 现代农业科技,2015, 649(11):94−96 doi: 10.3969/j.issn.1007-5739.2015.11.054 GAO X J, LANG D Y, ZHOU L, et al. Effects of NaCl stress on the content and accumulation of active components in Bupleurum chinense DC[J]. Modern Agricultural Science and Technology,2015,49(11):94−96. doi: 10.3969/j.issn.1007-5739.2015.11.054
[22]	齐琪, 马书荣, 徐维东, 等. 盐胁迫对植物生长的影响及耐盐生理机制研究进展[J]. 分子植物育种,2020,18(8):2741−2746 doi: 10.13271/j.mpb.018.002741 QI Q, MA S R, XU W D, et al. Effects of salt stress on plant growth and research progress on physiological mechanism of salt tolerance[J]. Molecular Plant Breeding,2020,18(8):2741−2746. doi: 10.13271/j.mpb.018.002741
[23]	ZHU H J, CHANG W Q, ZHOU C G, et al. Chemicalome and metabolome profiling of chai-gui decoction using an integrated strategy based on UHPLC-Q-TOF-MS/MS analysis[J]. Journal of Chromatography B,2021,1185:122979. doi: 10.1016/j.jchromb.2021.122979
[24]	梁军, 夏永刚, 郭信东, 等. 超高效液相色谱-高分辨质谱法同时测定类叶牡丹中9种三萜皂苷类成分[J]. 中草药,2017,48(20):4323−4327 doi: 10.7501/j.issn.0253-2670.2017.20.029 LIANG J, XIA Y G, GUO X D, et al. Simultaneous determination of nine triterpenoid saponins in ye mudan-like compounds by ultra performance liquid chromatography-high resolution mass spectrometry[J]. Chinese Herbal Medicine,2017,48(20):4323−4327. doi: 10.7501/j.issn.0253-2670.2017.20.029
[25]	韩君. 白扁豆总皂苷的含量测定与成分分析[J]. 安徽农业科学,2021,49(8):195−198 doi: 10.3969/j.issn.0517-6611.2021.08.051 HAN J. Content determination and analysis of total saponins in white lentil[J]. Anhui Agricultural Sciences,2021,49(8):195−198. doi: 10.3969/j.issn.0517-6611.2021.08.051
[26]	李婧, 马小兵, 沈杰, 等. 基于文献挖掘与分子对接技术的抗新型冠状病毒中药活性成分筛选[J]. 中草药,2020,51(4):845−850 doi: 10.7501/j.issn.0253-2670.2020.04.003 LI J, MA X B, SHEN J, et al. Screening of active components of anti-novel coronavirus traditional chinese medicine based on literature mining and molecular docking technology[J]. Chinese Herbal Medicine,2020,51(4):845−850. doi: 10.7501/j.issn.0253-2670.2020.04.003
[27]	赵灵改, 吕学泽, 刘毅, 等. 黄芪中皂苷类成分的研究进展[J]. 食品安全质量检测学报,2021,12(12):4937−4946 doi: 10.19812/j.cnki.jfsq11-5956/ts.2021.12.034 ZHAO L G, LÜ X Z, LIU Y, et al. Research progress of saponins in Astragalus membranaceus[J]. Journal of Food Safety and Quality Inspection,2021,12(12):4937−4946. doi: 10.19812/j.cnki.jfsq11-5956/ts.2021.12.034
[28]	申佩瑶, 张也, 李玉钦, 等. 竹节参生物活性与质量控制研究进展[J]. 食品与机械,2021,37(11):211−220 doi: 10.13652/j.issn.1003-5788.2021.11.037 SHEN P Y, ZHANG Y, LI Y Q, et al. Research progress of saponins in Astragalus membranaceus[J]. Food and Machinery,2021,37(11):211−220. doi: 10.13652/j.issn.1003-5788.2021.11.037
[29]	陈禹汐, 于寒松, 王敏, 等. 大豆皂苷的研究进展与应用[J]. 食品工业科技,2021,42(21):420−427 doi: 10.13386/j.issn1002-0306.2020090316 CHEN Y X, YU H S, WANG M, et al. Research progress and application of soybean saponin[J]. Food Industry Science and Technology,2021,42(21):420−427. doi: 10.13386/j.issn1002-0306.2020090316
[30]	MAI E K, RADWAN A M, MOHAMED T M, et al. Cytotoxic effect of soybean saponin against colon cancer[J]. Biological Science and Medicine,2019(7):007.
[31]	高丽娜, 周长征, 刘青芝, 等. 远志皂苷类化合物及其药理作用研究进展[J]. 北京联合大学学报,2022,36(3):58−64 GAO L N, ZHOU C Z, LIU Q Z, et al. Research progress of polygala tenuifolia saponins and their pharmacological effects[J]. Journal of Beijing Union University,2022,36(3):58−64.