Study on Process Optimization and Immunomodulatory Effect of <i>Dendrobium</i> <i>Huoshanense</i> Tea

HUANG Ke; LIN Qiyan; YANG Ying; HOU Tingting; HAN Bangxing; LIU Dong

doi:10.13386/j.issn1002-0306.2022120003

Volume 44 Issue 20

Oct. 2023

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Science and Technology of Food Industry > 2023 > 44(20): 213-220. > DOI: 10.13386/j.issn1002-0306.2022120003

HUANG Ke, LIN Qiyan, YANG Ying, et al. Study on Process Optimization and Immunomodulatory Effect of Dendrobium Huoshanense Tea[J]. Science and Technology of Food Industry, 2023, 44(20): 213−220. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022120003.

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Study on Process Optimization and Immunomodulatory Effect of Dendrobium Huoshanense Tea

HUANG Ke^{1, 2,},
LIN Qiyan^{2, 3},
YANG Ying^{1, 2},
HOU Tingting^{1, 2},
HAN Bangxing^{2, 3},
LIU Dong^{2, 3, ,}

1.
College of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China
2.
Anhui Ecological Agriculture Engineering Research Center of Traditional Chinese Medicine, Liuan 237012, China
3.
College of Biological and Pharmaceutical Engineering, West Anhui University, Liuan 237012, China

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Received Date: December 04, 2022
Available Online: August 01, 2023

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Abstract

Abstract

To explore the synergisic effect of DH (Dendrobium huoshanense) and KBT (Keemun black tea) on preventing immunity reduction. The solid-liquid ratio, extraction time and extraction times of DH and KBT were studied by single factor test and orthogonal test with the extraction rate of Dendrobium polysaccharide and black tea polyphenols as indexes, the extraction techniques of DH and Keemun black tea were optimized respectively. The optimal ratio of KBT and DH was determined by toxicity test and phagocytosis test on RAW264.7 cells. The results showed that the optimum extraction conditions of DH polysaccharide were as follows: The ratio of material to liquid was 1:80 (g/mL), the extraction time was 80 min, and the extraction times were 4 times. The optimum extraction conditions of KBT polyphenols were as follows: The ratio of material to liquid was 1:80 (g/mL), the extraction time was 25 min, and the extraction times were 5 times. The ratio of KBT to DH was determined by observing the effect of different proportions of the mixture on macrophages. The results of the in vitro experiment showed that the extract mixture of 75% KBT and 25% DH had the best immune-enhancing effect. The results of the in vivo test showed that the high-dose group of KTDH could significantly reduce the changes of levels of cytokines IL-2 and IL-6 due to immunosuppression in mice (P<0.01). To sum up, DH and KBT can synergistically enhance immunity and prevent immune decline, which can provide new ideas for the development and application of DH and KBT.
- Dendrobium huoshanense,
- Keemun black tea,
- extraction process,
- immunoregulation

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References

[1]	ZHA X Q, ZHAO H W, BANSAL V, et al. Immunoregulatory activities of Dendrobium huoshanense polysaccharides in mouse intestine, spleen and liver[J]. International Journal Of Biological Macromolecules,2014,64:377−82. doi: 10.1016/j.ijbiomac.2013.12.032
[2]	SI H Y, CHEN N F, CHEN N D, et al. Structural characterisation of a water-soluble polysaccharide from tissue-cultured Dendrobium huoshanense C.Z. Tang et S.J. Cheng[J]. Natural Product Research,2018,32(3):252−60. doi: 10.1080/14786419.2017.1350670
[3]	SHANG Z Z, QIN D Y, LI Q M, et al. Dendrobium huoshanense stem polysaccharide ameliorates rheumatoid arthritis in mice via inhibition of inflammatory signaling pathways[J]. Carbohydrate Polymers,2021,258:117657. doi: 10.1016/j.carbpol.2021.117657
[4]	WAN J, GONG X, WANG F, et al. Comparative analysis of chemical constituents by hplc-esi-ms(n) and antioxidant activities of Dendrobium huoshanense and dendrobium officinale[J]. Biomedical Chromatography,2022,36(1):e5250.
[5]	LIN J, CHANG Y J, YANG W B, et al. The multifaceted effects of polysaccharides isolated from Dendrobium huoshanense on immune functions with the induction of interleukin-1 receptor antagonist (IL-1ra) in monocytes[J]. PLoS One,2014,9(4):e94040. doi: 10.1371/journal.pone.0094040
[6]	LEE C T, KUO H C, CHEN Y H, et al. Current advances in the biological activity of polysaccharides in dendrobium wit source[J]. Current Medicinal Chemistry,2017,24:1−19. doi: 10.2174/092986732401170116154528
[7]	李囡囡, 周远远. 霍山石斛药理学研究及其在保健食品中的应用进展[J]. 宜春学院学报,2022,44(6):25−27, 107. [LI N N, ZHOU Y Y. Pharmacological research of dendrobium huoshanense and its application in health food[J]. Journal of Yichun College,2022,44(6):25−27, 107. Li N N, ZHOU Y Y. Pharmacological research of dendrobium huoshanense and its application in health food [J]. Journal of Yichun College, 202, 44(6): 25-27+107.
[8]	GAO Y, XU Y, RUAN J, et al. Selenium affects the activity of black tea in preventing metabolic syndrome in high-fat diet-fed Sprague-Dawley rats[J]. Journal of The Science of Food And Agriculture,2020,100(1):225−34. doi: 10.1002/jsfa.10027
[9]	DAS A S, DAS D, MUKHERJEE M, et al. Phytoestrogenic effects of black tea extract (Camellia sinensis) in an oophorectomized rat (Rattus norvegicus) model of osteoporosis[J]. Life Sciences,2005,77(24):3049−57. doi: 10.1016/j.lfs.2005.02.035
[10]	HAYAT K, IQBAL H, MALIK U, et al. Tea and its consumption: Benefits and risks[J]. Critical Reviews in Food Science and Nutrition,2015,55(7):939−954.
[11]	AHAMMED G J, LI X. Hormonal regulation of health-promoting compounds in tea (Camellia sinensis L.)[J]. Plant Physiology and Biochemistry,2022,185:390−400. doi: 10.1016/j.plaphy.2022.06.021
[12]	SUN L, ZHANG Y, ZHANG W, et al. Green tea and black tea inhibit proliferation and migration of HepG2 cells via the pi3k/akt and mmps signalling pathway[J]. Biomed Pharmacotherapy,2020,125:109893. doi: 10.1016/j.biopha.2020.109893
[13]	SINGH B N, RAWAT A K, BHAGAT R M, et al. Black tea: Phytochemicals, cancer chemoprevention, and clinical studies[J]. Critical Reviews in Food Science and Nutrition,2017,57(7):1394−1410. doi: 10.1080/10408398.2014.994700
[14]	KHAN N, MUKHTAR H. Tea polyphenols in promotion of human health[J]. Nutrients,2018,11(1):39. doi: 10.3390/nu11010039
[15]	WANG S, LI Z, MA Y, et al. Immunomodulatory effects of green tea polyphenols[J]. Molecules,2021,26(12):3755. doi: 10.3390/molecules26123755
[16]	ZHANG L, SANTOS J S, CRUZ T M, et al. Multivariate effects of Chinese keemun black tea grades (Camellia sinensis var. sinensis) on the phenolic composition, antioxidant, antihemolytic and cytotoxic/cytoprotection activities[J]. Food Research International,2019,125:108516. doi: 10.1016/j.foodres.2019.108516
[17]	WEISKOPF D, WEINBERGER B, GRUBECK-LOEBENSTEIN B. The aging of the immune system[J]. Transplant International,2009,22(11):1041−1050. doi: 10.1111/j.1432-2277.2009.00927.x
[18]	HILL Q A, NEWLAND A C. Fatigue in immune thrombocytopenia[J]. Br J Haematol,2015,170(2):141−9. doi: 10.1111/bjh.13385
[19]	李晓萌, 李佳惠, 王雨行, 等. 响应面优化莲藕多酚提取工艺及其生物活性研究[J]. 食品科技,2022,47(1):203−211. [LI X M, LI J H, WANG Y X, et al. Optimization of extraction process and bioactivities of polyphenols from lotus root by response surface method[J]. Food Sci Technol,2022,47(1):203−211. doi: 10.3969/j.issn.1005-9989.2022.1.spkj202201031 LI X M, LI J H, WANG Y X, et al. Optimization of extraction process and bioactivities of polyphenols from lotus root by response surface method [J]. Food Sci Technol, 2022, 47(1): 203-211. doi: 10.3969/j.issn.1005-9989.2022.1.spkj202201031
[20]	田先娇, 罗雪维, 杨新周, 等. 不同炮制方式对黄精有效成分含量的影响[J]. 化学试剂,2021,43(6):790−794. [TIAN X J, LUO X W, YANG X Z, et al. The effects of different processing methods on components in Polygonatum kingianum Coll. et Hemsl[J]. Chem Reagents,2021,43(6):790−794. doi: 10.13822/j.cnki.hxsj.2021007907 TIAN X J, LUO X W, YANG X Z, et al. The effects of different processing methods on components in polygonatum kingianum coll. et Hemsl [J]. Chem Reagents, 2021, 43(6): 790-794. doi: 10.13822/j.cnki.hxsj.2021007907
[21]	贾小演, 赵晓歌, 谢丹等. 蘘荷根茎乙醇提取物对LPS诱导巨噬细胞RAW264.7炎症反应的影响[J]. 中药药理与临床,2023,39(3):71−77. [JIA X Y, ZHAO X G, XIE D, et al. Effects of ethanol extract from rhizome of Yanghe rhizome on lps-induced inflammatory response of macrophage RAW264.7[J]. Pharmacology and Clinics of Chinese Materia Medica,2023,39(3):71−77. JIA X Y, ZHAO X G, XIE D, et al. Effects of ethanol extract from rhizome of Yanghe rhizome on lps-induced inflammatory response of macrophage RAW264.7[J]. Pharmacology and Clinics of Chinese Materia Medica, 2023, 39（3）: 71-77
[22]	KING W, TOLER K, WOODELL-MAY J. Role of white blood cells in blood- and bone marrow-based autologous therapies[J]. Biomed Research International,2018,2018:6510842.
[23]	LOCATI M, CURTALE G, MANTOVANI A. Diversity, mechanisms, and significance of macrophage plasticity[J]. Annual Review of Pathology-mechanisms of Disease,2020,15:123−47. doi: 10.1146/annurev-pathmechdis-012418-012718
[24]	FANG X, HENAO-MEJIA J, HENRICKSON S E. Obesity and immune status in children[J]. Current Opinion In Pediatrics,2020,32(6):805−15. doi: 10.1097/MOP.0000000000000953
[25]	LIN Q S. Mitochondria and apoptosis[J]. Sheng Wu Hua Xue Yu Sheng Wu Wu LI Xue Bao,1999,10(3−4):116−118.
[26]	LI F, CUI S H, ZHA X Q, et al. Structure and bioactivity of a polysaccharide extracted from protocorm-like bodies of dendrobium huoshanense[J]. International Journal of Biological Macromolecules,2015,72:664−72. doi: 10.1016/j.ijbiomac.2014.08.026
[27]	申羽佳, 李卫国, 张惠, 等. 绿茶多酚对小鼠皮肤伤口愈合时白介素-1β表达的影响[J]. 分子细胞生物学报,2009,42(Z1):179−185. [SHEN Y J, LI W G, ZHANG H, et al. Effect of green tea polyphenols on the expression of interleukin-1β during skin wound healing in mice[J]. Journal of Molecular Cell Biology,2009,42(Z1):179−185. SHEN Y J, LI W G, ZHANG H, et al. Effect of green tea polyphenols on the expression of interleukin-1β during skin wound healing in mice [J]. Journal of Molecular Cell Biology, 2009, 42(Z1): 179-185.
[28]	SARKAR A A, ALLYN D M, DELAY R J, et al. Cyclophosphamide-induced inflammation of taste buds and cytoprotection by amifostine [J]. Chem Senses, 2021, 46: 1−10.

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