Optimization of Alcohol Extraction Process of <i>Platycodon grandiflorum</i> by Box-Behnken Design-Response Surface Method and Analysis of Whitening Activity of Alcohol Extract

CAI Tiequan; PANG Huina; HUANG Yiqing; WAN Zhiqiang; YAN Mingming

doi:10.13386/j.issn1002-0306.2022110203

Volume 44 Issue 20

Oct. 2023

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2023 > 44(20): 189-196. > DOI: 10.13386/j.issn1002-0306.2022110203

CAI Tiequan, PANG Huina, HUANG Yiqing, et al. Optimization of Alcohol Extraction Process of Platycodon grandiflorum by Box-Behnken Design-Response Surface Method and Analysis of Whitening Activity of Alcohol Extract[J]. Science and Technology of Food Industry, 2023, 44(20): 189−196. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022110203.

Citation:

PDF (1917 KB)

Optimization of Alcohol Extraction Process of Platycodon grandiflorum by Box-Behnken Design-Response Surface Method and Analysis of Whitening Activity of Alcohol Extract

1.
Food Evaluation Center of the State Administration of Market Supervision and Administration, Beijing 100070, China
2.
Northeast Asia Institute of Chinese Medicine, Changchun University of Traditional Chinese Medicine, Changchun 130117, China
3.
Provincial Health Food Science and Technology Innovation Center, Changchun 130117, China

More Information

Received Date: November 16, 2022
Available Online: July 24, 2023

Graphical Abstract

Abstract

Abstract

To optimize the alcohol extraction process of Platycodon grandiflorum by response surface methodology, then investigate its whitening activity. With extracting time, extracting times and solvent volumn as factors, the ethanol extraction process of Platycodon grandiflorum was optimized by single factor investigation and Box-Behnken response surface design. The optimal ethanol extracting technology was 8 times of solvent, extraction for 3 times, 1 h each time. The extraction rates of platycodon D, arbutin, and total saponins in the ethanol extract were 0.122%±0.003%, 0.128%±0.005%, and 0.582%±0.007%, respectively, and the extract had good tyrosinase inhibition activity (92.39%), hyaluronidase inhibition activity (88.26%), cell melanin inhibition effect, and DPPH· (90.19%), ABTS⁺ (80.57%) free radical scavenging activity. The optimal ethanol extraction process of Platycodon grandiflorum was stable, reliable and operable. The ethanol extract had good whitening and antioxidant activity, which would provide scientific basis for further research on biological activity and whitening mechanism of Platycodon grandiflorum.
- Platycodon grandiflorum,
- alcohol extract,
- extraction process,
- tyrosinase,
- hyaluronidase,
- melanin

FullText(HTML)

References (34)

References

[1]	左军, 尹柏坤, 胡晓阳. 桔梗化学成分及现代药理研究进展[J]. 辽宁中医药大学学报,2019,21(1):113−116. [ZUO J, YIN B K, HU X Y. Progress in the chemical composition and modern pharmacological studies of Platycodon grandiflorum[J]. Journal of Liaoning University of Traditional Chinese Medicine,2019,21(1):113−116. ZUO J, YIN B K, HU X Y. Progress in the chemical composition and modern pharmacological studies of Platycodon grandiflorum [J]. Journal of Liaoning University of Traditional Chinese Medicine, 2019, 21 (1): 113-116.
[2]	李时珍. 本草纲目（校点本上、下册）[M]. 北京: 人民卫生出版社, 2004: 714 LI S Z. Compendium of materia medica (the first and second volumes)[M]. Beijing: People's Health Publishing House, 2004: 714.
[3]	董双勇, 徐远胜, 王弋, 等. 桔梗总皂苷对大鼠急性肺损伤保护作用及机制[J]. 中华急诊医学杂志,2017,26(12):1413−1417. [DONG S Y, XU Y S, WANG Y, et al. Protective effects and mechanisms of acute lung injury in rats[J]. Chinese Journal of Emergency Medicine,2017,26(12):1413−1417. doi: 10.3760/cma.j.issn.1671-0282.2017.12.016 DONG S Y, XU Y S, WANG Y, et al. Protective effects and mechanisms of acute lung injury in rats [J]. Chinese Journal of Emergency Medicine, 2017, 26 (12): 1413-1417. doi: 10.3760/cma.j.issn.1671-0282.2017.12.016
[4]	姚琳, 张俊威, 孟庆杰, 等. 桔梗总皂苷对PM2.5致肺损伤大鼠表面活性物质相关蛋白A表达的影响[J]. 中国药业,2018,27(1):17−20. [YAO L, ZHANG J W, MENG Q J, et al. Effect of total saponin on the expression of surfactant-related protein A in PM2.5-induced lung injury rats[J]. China Pharmaceutical Company,2018,27(1):17−20. YAO L, ZHANG J W, MENG Q J, et al. Effect of total saponin on the expression of surfactant-related protein A in PM2.5-induced lung injury rats [J]. China Pharmaceutical Company, 2018, 27 (1): 17-20.
[5]	梁仲远. 桔梗水提液的镇咳、祛痰作用研究[J]. 中国药房,2011,22(35):3291−3292. [LIANG Z Y. Study on cough and expectorant effects of aqueous extract of C. platycodon[J]. Pharmacy of China,2011,22(35):3291−3292. LIANG Z Y. Study on cough and expectorant effects of aqueous extract of C. platycodon [J]. Pharmacy of China, 2011, 22 (35): 3291-3292.
[6]	邓亚羚, 任洪民, 叶先文, 等. 桔梗的炮制历史沿革、化学成分及药理作用研究进展[J]. 中国实验方剂学杂志,2020,26(2):190−202. [DENG Y L, REN H M, YE X W, et al. Processing history, chemical composition and pharmacological effects of Platycodon grandiflorum[J]. Chinese Journal of Experimental Medicine,2020,26(2):190−202. DENG Y L, REN H M, YE X W, et al. Processing history, chemical composition and pharmacological effects of Platycodon grandiflorum [J]. Chinese Journal of Experimental Medicine, 2020, 26 (2): 190-202.
[7]	田雨弘. 桔梗的化学成分分析及药理活性研究[D]. 长春: 吉林农业大学, 2017 TIAN Y H. Chemical composition analysis and pharmacological activity study of Platycodon grandiflorum [D]. Changchun: Jilin Agricultural University, 2017.
[8]	朴向民, 于营, Han Sin-Hee, 等. 桔梗的体外抗氧化活性及总多酚和黄酮苷元含量分析[J]. 吉林农业大学学报,2017,39(5):579−584. [PU X M, YU Y, HAN S H, et al. In vitro antioxidant activity and element content of total polyphenols and flavonoids[J]. Journal of Jilin Agricultural University,2017,39(5):579−584. PU X M, YU Y, HAN S H, et al. In vitro antioxidant activity and element content of total polyphenols and flavonoids [J]. Journal of Jilin Agricultural University, 2017, 39 (5): 579-584.
[9]	周勇. 桔梗研究进展[J]. 世界最新医学信息文摘,2017,17(39):19, 22. [ZHOU Y. Progress in Platycodon grandiflorum[J]. World's Latest Medical Information Abstract,2017,17(39):19, 22. ZHOU Y. Progress in Platycodon grandiflorum [J]. World's Latest Medical Information Abstract, 2017, 17 (39): 19, 22.
[10]	仉劲, 李国清, 毕研文, 等. 中药材桔梗中常量元素和微量元素含量分析[J]. 农学学报,2017,7(7):43−46. [ZHANG J, LI G Q, BI Y W, et al. Analysis of constant elements and trace elements in Platycodon grandiflorum[J]. Journal of Agriculture,2017,7(7):43−46. ZHANG J, LI G Q, BI Y W, et al. Analysis of constant elements and trace elements in Platycodon grandiflorum [J]. Journal of Agriculture, 2017, 7 (7): 43-46.
[11]	KIM Y S, PARK C S, SUN N. ChaePotential of glycoside hydrolases treatment for production of balloon flower (Platycodon grandiflorum) extract[J]. Journal of Chitin and Chitosan,2022,27(3):132−136. doi: 10.17642/jcc.27.3.3
[12]	乔彩虹, 孟祥顺. 桔梗多糖降血糖作用及其机制[J]. 中国老年学杂志,2015,35(7):1944−1946. [QIAO C H, MENG X S. The hypoglycemic effect of Platycodon grandiflorum polysaccharide and its mechanism[J]. Chinese Journal of Gerontology,2015,35(7):1944−1946. QIAO C H, MENG X S. The hypoglycemic effect of Platycodon grandiflorum polysaccharide and its mechanism [J]. Chinese Journal of Gerontology, 2015, 35 (7): 1944-1946.
[13]	JUNG S, JOON A S, YEONGIN K, et al. Pharmacological effects and pharmacokinetic properties of Panax ginseng and Platycodon grandiflorum[J]. Korean Journal of Crop Science,2022,67(4):305−318.
[14]	单进军, 杨瑞, 张新庄, 等. 桔梗汤止咳祛痰的网络药理学研究[J]. 中草药,2018,49(15):3501. [SHAN J J, YANG R, ZHANG X Z, et al. Network pharmacology study of cough and expectorant in Tangycodon decoction[J]. Chinese Herbal Medicine,2018,49(15):3501. SHAN J J, YANG R, ZHANG X Z, et al. Network pharmacology study of cough and expectorant in Tangycodon decoction [J]. Chinese Herbal Medicine, 2018, 49 (15): 3501.
[15]	何毅帆, 龙芸鸾, 李征, 等. 天然植物美白成分作用机理的研究进展[J]. 日用化学品科学,2022,45(10):37−43. [HE Y F, LONG Y L, LI Z, et al. Progress on the mechanism of whitening components of natural plants[J]. Science of Daily Chemicals,2022,45(10):37−43. HE Y F, LONG Y L, LI Z, et al. Progress on the mechanism of whitening components of natural plants [J]. Science of Daily Chemicals, 2022, 45 (10): 37-43.
[16]	WANG B, AN X H, QU L P, et al. Review on oral plant extracts in skin whitening[J]. Food Science and Technology,2022,42:e83922. doi: 10.1590/fst.83922
[17]	ZHAO W, YANG A Q, WANG J, et al. Potential application of natural bioactive compounds as skin-whitening agents: A review[J]. Journal of Cosmetic Dermatology,2022,21(12):6669−6687. doi: 10.1111/jocd.15437
[18]	马馨桐, 邵帅, 张荣榕, 等. 桔梗美白活性部位的提取分离及活性初步研究[J]. 时珍国医国药,2021,32(10):2388−2392. [MA X T, SHAO S, ZHANG RR, et al. Preliminary study on extraction and activity of whitening active site of Platycodon grandiflorus[J]. Shizhen National Chinese Medicine,2021,32(10):2388−2392. doi: 10.3969/j.issn.1008-0805.2021.10.21 MA X T, SHAO S, ZHANG RR, et al. Preliminary study on extraction and activity of whitening active site of platgrandiflorum [J]. Shizhen National Chinese Medicine, 2021, 32 (10): 2388-2392. doi: 10.3969/j.issn.1008-0805.2021.10.21
[19]	但汉龙, 李娜, 叶红莲, 等. 响应面优化无患子皂苷超声辅助提取工艺[J]. 中南林业科技大学学报,2015,35(6):107−113. [DAN H L, LI N, YE H L, et al. The response surface optimized the ultrasound-assisted extraction process[J]. Journal of Central South University of Forestry and Technology,2015,35(6):107−113. DAN H L, LI N, YE H L, et al. The response surface optimized the ultrasound-assisted extraction process [J]. Journal of Central South University of Forestry and Technology, 2015, 35 (6): 107-113.
[20]	彭明. 星点设计-响应面法优化沙棘总黄酮提取工艺[J]. 中国药师,2020,23(7):1322−1326. [PENG M. Star design-response surface method to optimize total flavonoids extraction process of sea seabuckthorn[J]. Chinese Pharmacist,2020,23(7):1322−1326. doi: 10.3969/j.issn.1008-049X.2020.07.017 PENG M. Star design-response surface method to optimize total flavonoids extraction process of sea seabuckthorn [J]. Chinese Pharmacist, 2020, 23 (7): 1322-1326. doi: 10.3969/j.issn.1008-049X.2020.07.017
[21]	李凡, 许琦, 熊建, 等. 响应面优化设计饮料专用酵母抽提物多糖提取工艺[J]. 食品科技,2020,45(4):164−168. [LI F, XU Q, XIONG J, et al. The response surface was optimized to design the polysaccharide extraction process for beverages[J]. Food Technology,2020,45(4):164−168. LI F, XU Q, XIONG J, et al. The response surface was optimized to design the polysaccharide extraction process for beverages [J]. Food Technology, 2020, 45 (4): 164-168.
[22]	许守超, 陈屠梦, 包绍印, 等. 基于响应面试验设计优选衢枳壳多指标成分工艺研究[J]. 中药材,2019,42(11):2617−2621. [XU S C, CHEN T M, BAO S Y, et al. Research on multi-index composition technology of C. aurantii based on response surface test design[J]. Traditional Chinese medicine,2019,42(11):2617−2621. XU S C, CHEN T M, BAO S Y, et al. Research on multi-index composition technology of C. aurantii based on response surface test design [J]. Traditional Chinese medicine, 2019, 42 (11): 2617-2621.
[23]	毛艳, 古丽白热木·玉素因, 蔡晓翠, 等. Box-Behnken响应面法结合层次分析法优化新疆紫草根的提取工艺[J]. 中药材,2019,42(7):1605−1610. [MAO Y, GULI BAI RE MU Y, CAI X C, et al. Box-Behnken response surface method combined with hierarchical analysis to optimize the extraction process of Xinjiang purple roots[J]. Chinese Medicinal Materials,2019,42(7):1605−1610. MAO Y, GULI BAI RE MU Y, CAI X C, et al. Box-Behnken response surface method combined with hierarchical analysis to optimize the extraction process of Xinjiang purple roots [J]. Chinese medicinal materials, 2019, 42 (7): 1605-1610.
[24]	MA X T, SHAO S, XIAO F Q, et al. Platycodon grandiflorum extract: Chemical composition and whitening, antioxidant, and anti-inflammatory effects[J]. RSC advances,2021,11(18):10814−10826. doi: 10.1039/D0RA09443A
[25]	JIAO B, XU C T, LI Q, et al. Chemical constituents of flavonoids from Camellia oleifera and their anti-inflammatory activity in vitro[J]. Proprietary Chinese Medicine,2019,41:327−333.
[26]	ELZBIETA W, ALDONA K, RENATA Z W. Antioxidant activity and free radicals of roasted herbal materials[J]. Herba Polonica,2017,63(2):34−41. doi: 10.1515/hepo-2017-0011
[27]	庞会娜, 董红影, 肖凤琴, 等. 响应面法优化葛根蛋白酶解工艺及其体外抗氧化特性分析[J]. 食品工业科技,2022,43(24):197−204. [PANG H N, DONG H Y, XIAO F Q, et al. Optimization of protedigestion process and in vitro antioxidant properties[J]. science and technology of Food industry,2022,43(24):197−204. PANG H N, DONG H Y, XIAO F Q, et al. Optimization of protedigestion process and in vitro antioxidant properties [J]. Food Industry Technology, 2022, 43 (24): 197-204.
[28]	张倩. 黄茶多糖的分离纯化及其抗氧化活性研究[J]. 食品研究与开发,2021,42(14):49−55. [ZHANG Q. Isolation and purification of yellow tea polysaccharides and their antioxidant activity[J]. Food Research and Development,2021,42(14):49−55. ZHANG Q. Isolation and purification of yellow tea polysaccharides and their antioxidant activity [J]. Food Research and Development, 2021, 42 (14): 49-55.
[29]	张猛猛. 玛咖根部多糖的结构鉴定及免疫调节活性研究[D]. 广州: 华南理工大学, 2019 ZHANG M M. Structural identification and immunomodulatory activity of maca root marcan[D]. Guangzhou: South China University of Technology, 2019.
[30]	AUNG N N, NGAWHIRUNPAT T, ROJANARATA T, et al. HPMC/PVP dissolving microneedles: A promising delivery platform to promote trans-epidermal delivery of alpha-arbutin for skin lightening[J]. AAPS PharmSciTech,2019,21(1):25.
[31]	陈虎, 侯丽娟, 路付勇, 等. 响应面法优化超声溶剂法提取甜菊糖苷工艺[J]. 食品工业,2022,43(5):6−9. [CHEN H, HOU L J, LU F Y, et al. Optimization of stevia glycoside extraction by response surface method[J]. Food Industry,2022,43(5):6−9. CHEN H, HOU L J, LU F Y, et al. Optimization of stevia glycoside extraction by response surface method [J]. Food Industry, 2022, 43 (5): 6-9.
[32]	夏禹, 张文珍, 黄真, 等. 温莪术药渣再提取工艺及其美白活性研究[J]. 天然产物研究与开发,2022,34(3):457−464. [XIA Y, ZHANG W Z, HUANG Z, et al. Study on the reextraction process and whitening activity of the residue[J]. Research and Development of Natural Products,2022,34(3):457−464. XIA Y, ZHANG W Z, HUANG Z, et al. Study on the reextraction process and whitening activity of the residue [J]. Research and Development of Natural Products, 2022, 34 (3): 457-464.
[33]	LEE J, KIM E, JI H, et al. Anti-melanogenesis and skin protective activities of Panax ginseng calyx ethanol extract[J]. J Ginseng Res,2018,42:389−399. doi: 10.1016/j.jgr.2018.02.007
[34]	邱金东, 汤昆. DPPH和ABTS法测定核桃仁的体外抗氧化活性[J]. 中成药,2008(8):1215−1216. [QIU J D, TANG K. In vitro antioxidant activity of walnut kernel by DPPH and ABTS[J]. Chinese Patent Medicine,2008(8):1215−1216. QIU J D, TANG K. In vitro antioxidant activity of walnut kernel by DPPH and ABTS [J]. Chinese patent medicine, 2008 (8): 1215-1216.