Optimization of the Preparation Process and Evaluation of the Activity of <i>β</i>-Glucan/Nano-selenium Complex (DNT-Se) from Black Fungus

HU Cheng; SONG Haoying; CHANG Cong; QIU Zhenpeng; ZHENG Guohua; MENG Yan

doi:10.13386/j.issn1002-0306.2022100004

Volume 44 Issue 21

Oct. 2023

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Science and Technology of Food Industry > 2023 > 44(21): 162-170. > DOI: 10.13386/j.issn1002-0306.2022100004

HU Cheng, SONG Haoying, CHANG Cong, et al. Optimization of the Preparation Process and Evaluation of the Activity of β-Glucan/Nano-selenium Complex (DNT-Se) from Black Fungus[J]. Science and Technology of Food Industry, 2023, 44(21): 162−170. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022100004.

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Optimization of the Preparation Process and Evaluation of the Activity of β-Glucan/Nano-selenium Complex (DNT-Se) from Black Fungus

1.
College of Pharmacy, Hubei University of Chinese Medicine, Wuhan 432500, China
2.
Key Laboratory of Chinese Medicine Resource and Compound Prescription, Hubei University of Chinese Medicine, Wuhan 432500, China

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Received Date: October 07, 2022
Available Online: September 05, 2023

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Abstract

Abstract

This study aimed to explore the optimal conditions for the preparation of β-glucan/nanosized selenium (Se NPs) complexes (DNT-Se) and determining the safety of DNT-Se. The concentration of polysaccharides (DNTs), the ratio of vitamin C (V_C) to Na₂SeO₃, the concentration of Na₂SeO₃, the reaction time, and the reaction temperature were used as experimental factors. The dual-wavelength colorimetric method and the Malvern particle-size assay method were used to characterize changes in the particle size of Se NPs in DNT-Se, and the single-factors and orthogonal experimental design were used to investigate the preparation process of DNT-Se, and the toxicity of DNT-Se to 293T human embryonic kidney cells and normal liver cells of AML-12 mice was evaluated by using CCK-8. The results showed that the optimal conditions for DNT-Se preparation were list as follows: 1.0 mg/mL of DNTs, and 1.0 mg/mL of Na₂SeO₃ with the ratio of vitamin C to Na₂SeO₃ 6:1. Moreover, the reaction time was 12 h, and the reaction temperature was 25 ℃. The mean diameter of DNT-Se prepared under such conditions was around 34.50 nm. Cell proliferation experiment results showed that DNT-Se was non-toxic to 293T human embryonic kidney cells and normal liver cells of AML-12 mice, but it could inhibit the proliferation of HepG2 cells, and the IC₅₀ value of DNT-Se for HepG2 cells at 72 h was 42.54 μg/mL. The synthesis process of DNT-Se was fixed in this work, and the obtained nanoparticles were with smaller size and uniform distribution. Moreover, the DNT-Se could significantly inhibit the proliferation of HepG2 hepatoma cells without toxicity to normal cells, which was of good biosafety.
- Se NPs,
- black fungus,
- β-glucans,
- process investigation

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