LIU Dejiang, GUO Lele, TIAN Lijuan, et al. Optimization of Extraction Process and Antibacterial Activity of Total Alkaloids from Three Kinds of Actinidia Stems[J]. Science and Technology of Food Industry, 2023, 44(16): 195−203. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022090262.
Citation: LIU Dejiang, GUO Lele, TIAN Lijuan, et al. Optimization of Extraction Process and Antibacterial Activity of Total Alkaloids from Three Kinds of Actinidia Stems[J]. Science and Technology of Food Industry, 2023, 44(16): 195−203. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022090262.

Optimization of Extraction Process and Antibacterial Activity of Total Alkaloids from Three Kinds of Actinidia Stems

More Information
  • Received Date: September 25, 2022
  • Available Online: June 19, 2023
  • The yield of total alkaloids from stems of Actinidia arguta (A. arguta), Actinidia polygama (A. polygama), and Actinidia kolomikta (A. kolomikta) was used as an index, and the optimal extraction conditions of liquid-solid ratio, ultrasonic power, ultrasonic time, and ethanol concentration were investigated. The inhibitory effects of total alkaloids from the stems of A. arguta, A. polygama, and A. kolomikta against Botrytis cinerea (BC), Colletotrichum gloeosporioides (CG), Alternaria alternata (AA), Corynespora cassiicola (CC) and Pseudomonas syringae pv. Actinidiae (Psa) were investigated. Results showed that, the optimal extraction conditions were an ethanol concentration of 92%, liquid-solid ratio of 24 mL/g, ultrasonic power of 300 W, and an ultrasonic time of 20 min. Under optimized conditions, the highest yields of total alkaloids were 0.41, 0.43 and 0.39 mg/g for A. arguta, A. polygama and A. kolomikta stems, respectively. The total alkaloids from A. arguta stems showed antibacterial activity against Psa, and its minimum inhibitory concentration (MIC) was 50 mg/L. The total alkaloids from the stems of A. polygama and A. kolomikta did not show antibacterial activity against Psa and those from A. arguta, A. polygama and A. kolomikta stems showed no antibacterial activity against BC, CG, AA, and CC. The results confirmed that all three Actinidia spp. stems contained alkaloids and showed inhibitory activity against Psa, providing a theoretical basis for subsequent in-depth studies on the total alkaloids of Actinidia spp.
  • [1]
    孙宁宁. 长白山野生软枣猕猴桃的成分分析及保鲜研究[D]. 吉林: 吉林农业大学, 2007.

    SUN N N. Component analyzes and refreshing stady on Actinidia arguta in Changbaishan[D]. Jilin: Jilin Agricultural University, 2007.
    [2]
    刘春涵, 刘汉柱, 辛华. 葛枣猕猴桃果实形态及营养成分含量分析[J]. 植物资源与环境学报,2014,23(3):117−118. [LIU C H, LIU H Z, XIN H. Analysis on morphology and content of nutrient components of Actinidia polygama fruit[J]. Journal of Plant Resources and Environment,2014,23(3):117−118.

    LIU C H, LIU H Z, XIN H. Analysis on morphology and content of nutrient components of Actinidia polygama fruit[J]. Journal of Plant Resources and Environment, 2014, 23(3): 117-118.
    [3]
    陆娟, 常清泉, 谭莉, 等. 响应面优化超声提取狗枣猕猴桃叶粗多糖研究[J]. 食品研究与开发,2017,38(22):65−69. [LU J, CHANG Q Q, TAN L, et al. Optimization of the ultrasonic-assisted extraction of polysaccharide from the leaves of Actinidia kolomikta using response surface methodology[J]. Food Research and Development,2017,38(22):65−69.

    LU J, CHANG Q Q, TAN L, et al. Optimization of the ultrasonic-assisted extraction of polysaccharide from the leaves of Actinidia kolomikta using response surface methodology[J]. Food Research And Development, 2017, 38(22): 65-69.
    [4]
    丁玉萍, 王梦泽, 刘宇欣, 等. 软枣猕猴桃产品开发及利用研究进展[J]. 食品与发酵工业,2023,49(06):308−314、323. [DING Y P, WANG M Z, LIU Y X, et al. Research progress on product development and utilization of Actinidia Arguta[J]. Food and Fermentation Industries,2023,49(06):308−314、323.

    DING Y P, WANG M Z, LIU Y X, et al. Research progress on product development and utilization of Actinidia Arguta[J]. Food and Fermentation Industries, 2023, 49(06): 308-314、323.
    [5]
    王业社, 杨贤均, 陈立军, 等. 湖南云山野生药用藤本植物资源[J]. 贵州农业科学,2014,42(4):29−35、41. [WANG Y S, YANG X J, CHEN L J, et al. Wild medicinal liana resources in hunan yun maintain[J]. Guizhou Agricultural Sciences,2014,42(4):29−35、41.

    WANG Y S, YANG X J, CHEN L J, et al. Wild medicinal liana resources in hunan yun maintain[J]. Guizhou Agricultural Sciences, 2014, 42(4): 29-35、41.
    [6]
    左丽丽, 高永欣, 张岚, 等. 狗枣猕猴桃多酚对肥胖小鼠的减肥降脂作用[J]. 粮食与油脂,2021,34(10):132−136. [ZUO L L, GAO Y X, ZHANG L, et al. Effects of Actinidia Kolomikta (Maxim & Rupr. ) Maxim polyphenols on reduce weight and lipid lowering in obese mice[J]. Cereals & Oils,2021,34(10):132−136.

    ZUO L L, GAO Y X, ZHANG L, et al. Effects of Actinidia Kolomikta (Maxim & Rupr. ) Maxim polyphenols on reduce weight and lipid lowering in obese mice[J]. Cereals & Oils, 2021, 3 4(10): 132-136.
    [7]
    刘旸旸. 软枣猕猴桃中生物碱的提取纯化及生物活性研究[D]. 沈阳: 沈阳农业大学, 2016.

    LIU Y Y. Extraction, Purification and biological activities of alkaloid from Actinidia auguta[D]. Shenyang: Shenyang Agricultural University, 2016.
    [8]
    刘梦韩. 葛枣猕猴桃化学成分的研究[D]. 吉林: 吉林大学, 2009.

    LIU M H. Study on the chemical constituents of Actinidia Polygama (Sieb. Et Zucc. ) Miq[D]. Jilin: Jilin University, 2009.
    [9]
    连政. 海拔对野生狗枣猕猴桃生长及生理生化指标的影响[D]. 吉林: 延边大学, 2021.

    LIAN Z. Effects of altitude on growth and physiological and biochemcal indexes of wild Actinidia kolomikta[D]. Jilin: Yanbian University, 2021.
    [10]
    LUO M L, HUANG W, ZHU H P, et al. Advances in indole-containing alkaloids as potential anticancer agents by regulating autophagy[J]. Biomedicine & Pharmacotherapy,2022,149:112827.
    [11]
    DHYANI P, QUISPE C, SHARMA E, et al. Anticancer potential of alkaloids: a key emphasis to colchicine, vinblastine, vincristine, vindesine, vinorelbine and vincamine[J]. Cancer Cell International,2022,22(1):206. doi: 10.1186/s12935-022-02624-9
    [12]
    OTHMAN L, SLEIMANl A, ABDEL-MASSIH R M. Antimicrobial activity of polyphenols and alkaloids in middle eastern plants[J]. Frontiers in Microbiology,2019,10:911. doi: 10.3389/fmicb.2019.00911
    [13]
    HUANG B, WANG J, HAN X B, et al. The relationship between material transformation, microbial community and amino acids and alkaloid metabolites in the mushroom residue-prickly ash seed oil meal composting with biocontrol agent addition[J]. Bioresource Technology,2022,350:12691−126913.
    [14]
    Li H, Shi H D, Xu P, et al. Metabolomics and microbiome reveal potential root microbiota affecting the alkaloidal metabolome in Aconitum vilmorinianum Kom[J]. BMC Microbiology,2022,22(1):70. doi: 10.1186/s12866-022-02486-1
    [15]
    WANG X Y, GAO H, MA Z C, et al. Two new alkaloids from Pachysandra terminalis and their antibacterial activity assessment[J]. Phytochemistry Letters,2022,48:23−27. doi: 10.1016/j.phytol.2022.01.014
    [16]
    朱海云, 李勃, 李燕, 等. 丁香假单胞菌猕猴桃致病变种的遗传多样性及进化关系[J]. 微生物学杂志,2013,33(4):66−71. [ZHU H Y, LI B, LI Y, et al. Relation of genetic diversity and evolution of kiwifruit pathogen Pseudomonas syringae pv. actinidiae[J]. Journal of Microbiology,2013,33(4):66−71.

    ZHU H Y, LI B, LI Y, et al. Relation of genetic diversity and evolution of kiwifruit pathogen Pseudomonas syringae pv. actinidiae[J]. Journal of Microbiology, 2013, 33(4): 66-71.
    [17]
    王海云. 永泰县猕猴桃细菌性溃疡病发生的原因与防治策略[J]. 植物医学,2022,1(3):113−118. [WANG H Y. Cause and control strategies of kiwifruit bacterial canker in Yongtai County[J]. Plant Health and Medicine,2022,1(3):113−118.

    WANG H Y. Cause and control strategies of kiwifruit bacterial canker in Yongtai County [J]. Plant Health and Medicine, 2022, 1(3): 113-118.
    [18]
    LEI Y H, JINGZ B, LI L. Election and evaluation of a new kiwifruit rootstock hybrid for bacterial canker resistance[J]. Acta Horticulturae,2015(1096):413−420.
    [19]
    赵奕彭. 软枣猕猴桃中生物碱的提取工艺优化、分离及抗氧化活性研究[D]. 沈阳: 沈阳农业大学, 2019.

    ZHAO Y P. Optimum extraction isolation of Actinidia arguta alkaloids, and antioxidant activity[D]. Shenyang: Shenyang Agricultural University, 2019.
    [20]
    陈静, 冷鹃, 杨喜爱, 等. 麻叶提取物对金黄色葡萄球菌的体外抑制初步研究[J]. 中国麻业科学,2018,40(1):17−21. [CHEN J, LENG J, YANG X A, et al. A preliminary study on antibacterial activities of bast fiber crop leaves extracts against S. aureus in vitro[J]. Plant Fiber Sciences in China,2018,40(1):17−21.

    CHEN J, LENG J, YANG X A, et al. A preliminary study on antibacterial activities of bast fiber crop leaves extracts against S. aureus in vitro[J]. Plant Fiber Sciences in China, 2018, 40(1): 17-21.
    [21]
    马玉楠. 黄花蒿和丁香挥发油抑制三七病害发生的研究[D]. 云南: 云南中医药大学, 2019.

    MA Y M. Study on the inhibitory effect of Artemisia annua and Syzygium aromaticum essential oil on the disease of Panax notoginseng[D]. Yunan: Yunnan University of Chinese Medicine, 2019.
    [22]
    张永欣, 朱童, 杨丹, 等. 黄连须根提取物生物碱类成分及抑菌活性研究[J]. 世界中医药,2021,16(17):2556−2561. [ZHANG Y X, ZHU T, YANG D, et al. Study on alkaloids and antibacterial activity of fibrous root extract of Coptis Chinensis[J]. World Chinese Medicine,2021,16(17):2556−2561.

    ZHANG Y X, ZHU T, YANG D, et al. Study on alkaloids and antibacterial activity of fibrous root extract of Coptis Chinensis[J]. World Chinese Medicine, 2021, 16(17): 2556-2561.
    [23]
    肖倩, 李守望, 梁晨, 等. 贝莱斯芽孢杆菌对黄瓜霜霉病的作用方式和持效期[J]. 农药,2021,60(11):829−831. [XIAO Q, LI S W, Liang C, et al. Action mode and duration of Bacillus velezensis against cucumber downy mildew[J]. Agrochemicals,2021,60(11):829−831.

    XIAO Q, LI S W, Liang C, et al. Action mode and duration of Bacillus velezensis against cucumber downy mildew[J]. Agrochemicals, 2021, 60(11): 829-831.
    [24]
    杜彦影, 刘长江, 刘旸旸. 响应面设计法优化软枣猕猴桃茎中总生物碱的提取工艺[J]. 食品工业科技,2015,36(13):220−224. [DU Y Y, LIU C J, LIU Y Y. Optimization of total alkaloids extraction from Actinidia arguta stems using response surface methodology[J]. Science and Technology of Food Industry,2015,36(13):220−224.

    DU Y Y, LIU C J, LIU Y Y. Optimization of total alkaloids extraction from Actinidia arguta stems using response surface methodology[J]. Science and Technology of Food Industry, 2015, 36(13): 220-224.
    [25]
    ZHANG X, WANG S, WU Q X, et al. Recovering high value-added anthocyanins from blueberry pomace with ultrasound-assisted extraction[J]. Food Chemistry: X,2022,16:100476. doi: 10.1016/j.fochx.2022.100476
    [26]
    LEI Y L, LI L L, LIU T T, et al. Development of sample preparation method for isoliquiritigenin, liquiritin,  and glycyrrhizic acid analysis in licorice by ionic liquids-ultrasound based extraction and high-performance liquid chromatography detection[J]. Food Chemistry,2013(138):173−179.
    [27]
    HUI X J, ZHEN L X, WEI P, et al. Berberine protects against diet-induced obesity through regulating metabolic endotoxemia and gut hormone levels[J]. Molecular Medicine Reports,2017,15(5):2765−2787. doi: 10.3892/mmr.2017.6321
    [28]
    裴沪荣, 卢明秀, 龙力, 等. 黄芩乙醇提取物对猕猴桃溃疡病致病菌抑菌机理研究[J]. 南方农业学报,2022,53(2):477−485. [PEI H R, LU M X, LONG L, et al. Primary antibacterial mechanism of ethanol extract of scutellaria baicalensis against the pathogens of kiwifruit canker[J]. Journal of Southern Agriculture,2022,53(2):477−485.

    PEI H R, LU M X, LONG L, et al. Primary antibacterial mechanism of ethanol extract of scutellaria baicalensis against the pathogens of kiwifruit canker[J]. Journal of Southern Agriculture, 2022, 53(2): 477-485.
    [29]
    NALIWAJSKI M R, WILENṄSKAl B, MISICKA A, et al. HPLC-PDA-ESI-HRMS-Based profiling of secondary metabolites of rindera graeca anatomical and hairy roots treated with drought and cold stress[J]. Cells,2022,11(6):931. doi: 10.3390/cells11060931
  • Related Articles

    [1]CHEN Wenlu, LIU Yanyan, GONG Yanni. Optimization of Fermentation Processing of Cheese Flavor Fermented Milk by Response Surface Methodology[J]. Science and Technology of Food Industry, 2021, 42(23): 235-242. DOI: 10.13386/j.issn1002-0306.2021040211
    [2]GUAN Jiaqi, KE Chuxin, HUANG He, LI Bailiang, JIAO Wenshu, LENG Youbin, HUO Guicheng. Optimization of Tendon Yogurt Jelly Formula by Response Surface Method[J]. Science and Technology of Food Industry, 2021, 42(3): 171-178. DOI: 10.13386/j.issn1002-0306.2020020156
    [3]LIN Hong-bin, FANG Jia-xing, BI Xiao-peng, YU Xiao-yu, HE Qiang, LIU Ping, DING Wen-wu, CHE Zhen-ming. Optimization of the Extraction Technique of Free Amino Acids from Pixian Board-bean Paste by Response Surface Methodology and Analysis of Their Taste Characteristics[J]. Science and Technology of Food Industry, 2019, 40(17): 56-63. DOI: 10.13386/j.issn1002-0306.2019.17.010
    [4]LV Kai-bo. Optimization of Lipase Hydrolysis of Safflower Seed Oil by Response Surface Methodology[J]. Science and Technology of Food Industry, 2018, 39(14): 76-80. DOI: 10.13386/j.issn1002-0306.2018.14.015
    [5]ZHOU Xiu-li, DONG Fu-kai, ZHA En-hui. Response surface optimization of pickled liquid formulation and quality determination of conditioning steak[J]. Science and Technology of Food Industry, 2017, (24): 194-200. DOI: 10.13386/j.issn1002-0306.2017.24.038
    [6]WANG Zhao-yu, CHI Xue-wen, BI Yan-hong, ZHAO Xiang-jie, FU Rui-ping, LI Ning-yang. Optimization of anti-browning technology of Agaricus bisporus by response surface methodology[J]. Science and Technology of Food Industry, 2017, (16): 167-171. DOI: 10.13386/j.issn1002-0306.2017.16.031
    [7]RAO Dan- hua, BAI Chan, GENG Sheng-rong, DENG Zi-hao, XU Chen, XIONG Guang-quan, ZU Xiao- yan, LI Hai-lan, JI Fei, WANG Bao-hua, LI Xin, LIAO Tao. Optimization of extraction process of sturgeon sperm protein by response surface methodology[J]. Science and Technology of Food Industry, 2017, (04): 272-277. DOI: 10.13386/j.issn1002-0306.2017.04.043
    [8]CHEN Wen-qiang, QIAO Yan-ming. Application of response surface for the optimization of liquid seed production process of Lentinus edodes[J]. Science and Technology of Food Industry, 2015, (18): 290-294. DOI: 10.13386/j.issn1002-0306.2015.18.050
    [9]BAO Yu-ting, WANG Wei-min, CHEN Su-hua, ZHONG Si-yan, YAO Wen-ting, ZHANG Ke. Optimization of smoked processing of tilapia through response surface method[J]. Science and Technology of Food Industry, 2015, (14): 275-281. DOI: 10.13386/j.issn1002-0306.2015.14.048
    [10]LIU Yan, FENG Yin, MO Yan, MIN Wei-hong, MA Jing-xi. Response surface optimization of fermentation medium for astaxanthin by pseudomonas[J]. Science and Technology of Food Industry, 2014, (20): 186-190. DOI: 10.13386/j.issn1002-0306.2014.20.032
  • Cited by

    Periodical cited type(1)

    1. 王小红,向东山,李文恒,汪鹏,张瑞刚. 响应面法优化沙棘葡萄果冻的制备工艺. 湖北民族大学学报(自然科学版). 2023(01): 33-39 .

    Other cited types(2)

Catalog

    Article Metrics

    Article views (146) PDF downloads (15) Cited by(3)

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return