Optimization of Extraction Process and Antibacterial Activity of Total Alkaloids from Three Kinds of <i>Actinidia</i> Stems

LIU Dejiang; GUO Lele; TIAN Lijuan; LI Li; YANG Tianzhu; ZHANG Huiming; LI Lili; XIA Jun

doi:10.13386/j.issn1002-0306.2022090262

Volume 44 Issue 16

Aug. 2023

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Science and Technology of Food Industry > 2023 > 44(16): 195-203. > DOI: 10.13386/j.issn1002-0306.2022090262

LIU Dejiang, GUO Lele, TIAN Lijuan, et al. Optimization of Extraction Process and Antibacterial Activity of Total Alkaloids from Three Kinds of Actinidia Stems[J]. Science and Technology of Food Industry, 2023, 44(16): 195−203. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022090262.

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Optimization of Extraction Process and Antibacterial Activity of Total Alkaloids from Three Kinds of Actinidia Stems

LIU Dejiang^{1, 2,},
GUO Lele^{1, 2},
TIAN Lijuan^{1, 2},
LI Li³,
YANG Tianzhu^{2, 4},
ZHANG Huiming^{2, 4},
LI Lili^{1, 2},
XIA Jun^{1, 2, ,}

1.
College of Biology and Agriculture, Jiamusi University, Jiamusi 154007, China
2.
China-Ukraine Agriculture & Forestry Technology Development and Application International Cooperation Joint Lab, Jiamusi 154007, China
3.
Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China
4.
College of Basic Medical, Jiamusi University, Jiamusi 154007, China

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Received Date: September 25, 2022
Available Online: June 19, 2023

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Abstract

Abstract

The yield of total alkaloids from stems of Actinidia arguta (A. arguta), Actinidia polygama (A. polygama), and Actinidia kolomikta (A. kolomikta) was used as an index, and the optimal extraction conditions of liquid-solid ratio, ultrasonic power, ultrasonic time, and ethanol concentration were investigated. The inhibitory effects of total alkaloids from the stems of A. arguta, A. polygama, and A. kolomikta against Botrytis cinerea (BC), Colletotrichum gloeosporioides (CG), Alternaria alternata (AA), Corynespora cassiicola (CC) and Pseudomonas syringae pv. Actinidiae (Psa) were investigated. Results showed that, the optimal extraction conditions were an ethanol concentration of 92%, liquid-solid ratio of 24 mL/g, ultrasonic power of 300 W, and an ultrasonic time of 20 min. Under optimized conditions, the highest yields of total alkaloids were 0.41, 0.43 and 0.39 mg/g for A. arguta, A. polygama and A. kolomikta stems, respectively. The total alkaloids from A. arguta stems showed antibacterial activity against Psa, and its minimum inhibitory concentration (MIC) was 50 mg/L. The total alkaloids from the stems of A. polygama and A. kolomikta did not show antibacterial activity against Psa and those from A. arguta, A. polygama and A. kolomikta stems showed no antibacterial activity against BC, CG, AA, and CC. The results confirmed that all three Actinidia spp. stems contained alkaloids and showed inhibitory activity against Psa, providing a theoretical basis for subsequent in-depth studies on the total alkaloids of Actinidia spp.
- Actinidia arguta,
- Actinidia polygama,
- Actinidia kolomikta,
- total alkaloids,
- antibacterial activity

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