Effects of Oviductus Ranae on Follicular Developmental Dysfunction by <i>Tripterygium</i> in Rats

Xueyuan SUN; Yu WANG; Xingyao HE; Lin DI; Xinyu LIU; Hongyu ZHAO; Fengqing ZHANG

doi:10.13386/j.issn1002-0306.2022090023

Volume 44 Issue 13

Jun. 2023

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2023 > 44(13): 398-406. > DOI: 10.13386/j.issn1002-0306.2022090023

SUN Xueyuan, WANG Yu, HE Xingyao, et al. Effects of Oviductus Ranae on Follicular Developmental Dysfunction by Tripterygium in Rats[J]. Science and Technology of Food Industry, 2023, 44(13): 398−406. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022090023.

Citation:

PDF (4793 KB)

Effects of Oviductus Ranae on Follicular Developmental Dysfunction by Tripterygium in Rats

Xueyuan SUN^{1, 2,},
Yu WANG²,
Xingyao HE^{1, 2},
Lin DI²,
Xinyu LIU²,
Hongyu ZHAO^2, ,,
Fengqing ZHANG^1, ,

1.
College of Chemistry and Life Sciences, Changchun University of Technology, Changchun 130012, China
2.
Pharmacodynamic and Toxicological Evaluation Center, Jilin Academy of Traditional Chinese Medicine Sciences, Changchun 130012, China

More Information

Received Date: September 04, 2022
Available Online: May 08, 2023

Graphical Abstract

Abstract

Abstract

Objective: To study the effect of oviductus ranae (OR) on follicle development and phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt) signaling pathway in the ovaries of follicular developmental dysfunction rats. Methods: Randomly divide the screened 40 female WISTAR rats into control group (CG), model group (MG), positive qroup (PG), OR high-dose (ORHG) and low-dose group (ORLG). Except the CG rats, the rats in other groups were treated with triptolide solution (40 mg/kg) once a day. The PG were treated with estradiol valerate solution (0.1 mg/kg) and megestrol acetate solution (0.8 mg/kg). The ORHG and ORLG were treated with OR solution (400、200 mg/kg). The rat serum was collected to determine the contents of estradiol (E2), progesterone (P), Luteinizing hormone (LH), Follicle-stimulating hormone (FSH) and testosterone (T) at 8th week . The ovaries and uterus of rats were harvested and weighed, and the organ index was calculated. HE staining and TUNEL staining were performed on one ovary to calculate the number of follicles at all levels. The relative expression of PI3K, AKT, phosphatase and tensin homolog deleted on chromosome ten (PTEN) mammalian target of rapamycin (mTOR) mRNA and the contents of PI3K, Akt, phosphorylated protein kinase B(p-Akt) protein in the other ovary was measured. Result: The results indicated that the follicular development dysfunction model was successfully established. Compared with the MG, the wet weight and index of uterus of the ORHG was increased significantly (P<0.05). The estrous cycle of rats in the ORHG and ORLG was significantly shortened (P<0.05), the number of secondary follicles was significantly increased (P<0.05), the follicular atresia rate was significantly reduced (P<0.05), the serum FSH and LH content was decreased significantly (P<0.05), the serum T and E2 content was increased significantly (P<0.05), PI3K protein content was increased significantly (P<0.05), Akt protein phosphorylation level was increased significantly (P<0.05), the relative expression of PTEN mRNA was decreased significantly (P<0.05). The relative expression of mTOR mRNA in ORHG was significantly lower than that of MG (P<0.05). Conclusion: OR can obviously improve the follicular development dysfunction of rats induced by triptolide, and it can up-regulate PI3K/Akt signal pathway to promote the growth and development of follicles in rats with follicular development dysfunction.

FullText(HTML)

References (25)

References

[1]	张熬, 张悦, 陈缘, 等. 东北林蛙油研究进展[J]. 养殖与饲料,2020(1):38−71. [ZHANG Ao, ZHANG Yue, CHEN Yuan, et al. Research progress of oviductus ranae in Northeast China[J]. Animals Breeding and Feed,2020(1):38−71. doi: 10.13300/j.cnki.cn42-1648/s.2020.01.025 ZHANG Ao, ZHANG Yue, CHEN Yuan, et al. Research progress of oviductus ranae in Northeast China[J]. Animals Breeding and Feed, 2020(1): 38-71. doi: 10.13300/j.cnki.cn42-1648/s.2020.01.025
[2]	郭宪一, 范红艳. 林蛙油的药理作用研究进展[J]. 吉林医药学院学报,2020,41(3):209−211. [GUO Xianyi, FAN Hongyan. Research progress on pharmacological effects of rana oil[J]. Journal of Jilin Medical University,2020,41(3):209−211. doi: 10.13845/j.cnki.issn1673-2995.2020.03.022 GUO Xianyi, FAN Hongyan. Research progress on pharmacological effects of rana oil[J]. Journal of Jilin Medical University, 2020, 41(3): 209-211. doi: 10.13845/j.cnki.issn1673-2995.2020.03.022
[3]	赵宏宇, 王玉, 孙雪缘, 等. 基于PI3K/Akt信号通路探讨哈蟆油对卵泡发育作用的影响[J]. 中国实验方剂学杂志,2021,27(21):121−130. [ZHAO Hongyu, WANG Yu, SUN Xueyuan, et al. Investigating the effect of oviductus ranae on follicle development based on PI3K/Akt signaling pathway[J]. Chinese Journal of Experimental Traditional Medical Formulae,2021,27(21):121−130. doi: 10.13422/j.cnki.syfjx.20212137 ZHAO Hongyu, WANG Yu, SUN Xueyuan, et al. Investigating the effect of oviductus ranae on follicle development based on PI3K/Akt signaling pathway[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2021, 27(21): 121-130. doi: 10.13422/j.cnki.syfjx.20212137
[4]	赵宏宇, 王玉, 刘新宇, 等. 林蛙油提升雌激素的机制研究[J]. 食品工业科技,2019,40(19):296−300. [ZHAO Hongyu, WANG Yu, SUN Xueyuan, et al. Study on the mechanism of oviductus ranae to elevate estrogen[J]. Science and Technology of Food Industry,2019,40(19):296−300. doi: 10.13386/j.issn1002-0306.2019.19.051 ZHAO Hongyu, WANG Yu, SUN Xueyuan, et al. Study on the mechanism of oviductus ranae to elevate estrogen[J]. Science and Technology of Food Industry, 2019, 40(19): 296-300. doi: 10.13386/j.issn1002-0306.2019.19.051
[5]	吴克明, 谌婕, 熊巍. 雷公藤多甙对雌性小鼠生殖功能影响的实验研究[J]. 中医研究,2007,20(4):28−33. [WU Keming, ZHAN Jie, XIONG Wei. Experimental study on the effects of Tripterygium wilfordii polyglycosides on reproductive function of female mice[J]. Traditional Chinese Medicinal Research,2007,20(4):28−33. doi: 10.3969/j.issn.1001-6910.2007.04.012 WU Keming, ZHAN Jie, XIONG Wei. Experimental study on the effects of Tripterygium wilfordii polyglycosides on reproductive function of female mice[J]. Traditional Chinese Medicinal Research, 2007, 20(4): 28-33. doi: 10.3969/j.issn.1001-6910.2007.04.012
[6]	曹俊岩. 雷公藤多苷致大鼠卵泡发育障碍模型的中医症候属性研究[D]. 成都: 成都中医药大学, 2011 CAO Junyan. Study on TCM symptom attributes of rat follicular development disorder model induced by Tripterygium wilfordii[D]. Chengdu: Chengdu University of Traditional Chinese Medicine, 2011.
[7]	白俊, 吴也可, 吴克明, 等. 雷公藤甲素通过 PI3K /AKT / mTOR通路诱导卵巢颗粒细胞自噬的实验研究[J]. 中国中药杂志,2019,44(16):3429−3434. [BAI Jun, WU Yeke, WU Keming, et al. Experimental study on triptolide-induced autophagy in ovarian granulosa cells via PI3K/AKT/mTOR pathway[J]. China Journal of Chinese Materia Medica,2019,44(16):3429−3434. BAI Jun, WU Yeke, WU Keming, et al. Experimental study on triptolide-induced autophagy in ovarian granulosa cells via PI3K/AKT/mTOR pathway[J]. China Journal of Chinese Materia Medica, 2019, 44(16): 3429-3434.
[8]	谌婕, 吴克明, 王家葵, 等. 雷公藤多苷致雌性小鼠肾虚生殖功能低下动物模型的研究[J]. 中药新药与临床药理,2007,18(3):208−211. [ZHAN Jie, WU Keming, WANG Jiahui, et al. Study on the animal model of kidney deficiency and reproductive dysfunction in female mice induced by Tripterygium wilfordii polyglycosides[J]. Traditional Chinese Drug Research and Clinical Pharmacology,2007,18(3):208−211. doi: 10.3321/j.issn:1003-9783.2007.03.013 ZHAN Jie, WU Keming, WANG Jiahui, et al. Study on the animal model of kidney deficiency and reproductive dysfunction in female mice induced by Tripterygium wilfordii polyglycosides[J]. Traditional Chinese Drug Research and Clinical Pharmacology, 2007, 18(3): 208-211. doi: 10.3321/j.issn:1003-9783.2007.03.013
[9]	张芳, 丁怡, 于潇, 等. 补肾养精颗粒调控早发性卵巢功能不全模型大鼠卵巢颗粒细胞凋亡机制研究[J]. 山东中医药大学学报,2022,46(3):365−372. [ZHANG Fang, DING Yi, YU Xiao, et al. Mechanism of Bushen Yangjing Granule in regulating apoptosis of ovarian granulosa cell in model rats with premature ovarian insufficiency[J]. Journal of Shandong University of Traditional Chinese Medicine,2022,46(3):365−372. doi: 10.16294/j.cnki.1007-659x.2022.03.015 ZHANG Fang, DING Yi, YU Xiao, et al. Mechanism of Bushen Yangjing Granule in regulating apoptosis of ovarian granulosa cell in model rats with premature ovarian insufficiency[J]. Journal of Shandong University of Traditional Chinese Medicine, 2022, 46(3): 365-372. doi: 10.16294/j.cnki.1007-659x.2022.03.015
[10]	张婷, 王颖, 王莉. 大鼠阴道涂片两种染色方法比较[J]. 中国比较医学杂志,2018,28(12):98−101. [ZHANG Ting, WANG Ying, WANG Li. Comparison of two staining methods for rat vaginal smears[J]. Chinese Journal of Comparative Medicine,2018,28(12):98−101. doi: 10.3969/j.issn.1671-7856.2018.12.016 ZHANG Ting, WANG Ying, WANG Li. Comparison of two staining methods for rat vaginal smears[J]. Chinese Journal of Comparative Medicine, 2018, 28(12): 98-101. doi: 10.3969/j.issn.1671-7856.2018.12.016
[11]	毛菁沁, 冒韵东. 原始卵泡激活与卵泡发育相关机制的研究进展[J]. 国际生殖健康/计划生育杂志,2020,39(3):225−228. [MAO Jingqin, MAO Yundong. Research progress on the mechanism of primordial follicle activation and follicle development[J]. Journal of International Reproductive Health/Family Planning,2020,39(3):225−228. doi: 10.3969/j.issn.1674-1889.2020.03.012 MAO Jingqin, MAO Yundong. Research progress on the mechanism of primordial follicle activation and follicle development[J]. Journal of International Reproductive Health/Family Planning, 2020, 39(3): 225-228. doi: 10.3969/j.issn.1674-1889.2020.03.012
[12]	吕文漪, 董晓英. 卵泡发育与卵巢早衰相关性的研究进展[J]. 医学研究杂志,2016,45(12):160−162. [LÜ Wenyi, DONG Xiaoying. Research progress on the relationship between follicular development and premature ovarian failure[J]. Journal of Medical Research,2016,45(12):160−162. LV Wenyi, DONG Xiaoying. Research progress on the relationship between follicular development and premature ovarian failure[J]. Journal of Medical Research, 2016, 45(12): 160-162.
[13]	ROLAKI A, DRAKAKIS P, MILLINGOS S, et al. Novel trends in follicular development, atresia and corpus luteum regression: A role for apoptosis[J]. Reprod Biomed Online,2005,11(1):93−103. doi: 10.1016/S1472-6483(10)61304-1
[14]	郝丽, 卢文, 林辉, 等. 雌孕激素替代治疗雷公藤所致卵巢早衰的疗效观察[J]. 中华肾脏病杂志,2005,21(3):143−145. [HAO Li, LU Wen, LIN Hui, et al. Efficacy of estrogen and progesterone replacement in the treatment of premature ovarian failure caused by Tripterygium wilfordii[J]. Chinese Journal of Nephrology,2005,21(3):143−145. doi: 10.3760/j.issn:1001-7097.2005.03.008 HAO Li, LU Wen, LIN Hui, et al. Efficacy of estrogen and progesterone replacement in the treatment of premature ovarian failure caused by Tripterygium wilfordii[J]. Chinese Journal of Nephrology, 2005, 21(3): 143-145. doi: 10.3760/j.issn:1001-7097.2005.03.008
[15]	付雨, 吴克. 雷公藤多苷对大鼠卵巢血管生成及血供的影响[J]. 四川中医,2011,29(6):50−52. [FU Yu, WU Ke. Effects of Tripterygium wilfordii polyglycosides on angiogenesis and blood supply in rat ovary[J]. Journal of Sichuan of Traditional Chinese Medicine,2011,29(6):50−52. FU Yu, WU Ke. Effects of Tripterygium wilfordii polyglycosides on angiogenesis and blood supply in rat ovary[J]. Journal of Sichuan of Traditional Chinese Medicine, 2011, 29(6): 50-52.
[16]	王桂玲, 任春娥, 王丽. 雷公藤多甙对雌性大鼠不良反应的实验研究[J]. 河北医药,2009,31(4):416−418. [WANG Guiling, REN Chune, WANG Li. Experimental study on adverse reactions of Tripterygium wilfordii polyglycosides in female rats[J]. Hebei Medical Journal,2009,31(4):416−418. doi: 10.3969/j.issn.1002-7386.2009.04.011 WANG Guiling, REN Chune, WANG Li. Experimental study on adverse reactions of Tripterygium wilfordii polyglycosides in female rats[J]. Hebei Medical Journal, 2009, 31(4): 416-418. doi: 10.3969/j.issn.1002-7386.2009.04.011
[17]	徐丽萍, 张蕾, 宋欣伟. 雷公藤多苷不同给药方式对雌性大鼠肝功能及生殖功能影响的比较研究[J]. 浙江中医药大学学报,2019,43(2):177−181. [XU Liping, ZHANG Lei, SONG Xinwei. Comparative study on the effects of different administration modes of Tripterygium wilfordii polyglycosides on liver function and reproductive function in female rats[J]. Journal of Zhejiang Chinese Medical University,2019,43(2):177−181. doi: 10.16466/j.issn1005-5509.2019.02.013 XU Liping, ZHANG Lei, SONG Xinwei. Comparative study on the effects of different administration modes of Tripterygium wilfordii polyglycosides on liver function and reproductive function in female rats[J]. Journal of Zhejiang Chinese Medical University, 2019, 43(2): 177-181. doi: 10.16466/j.issn1005-5509.2019.02.013
[18]	RICHARD S, BALTZ J M. Prophase I Arrest of mouse oocytes mediated by natriuretic peptide precursor c requires gja1 (connexin-43) andgja4 (connexin-37) gap Junctions in the antral follicle and cumulus-oocyte complex[J]. Biol Reprod,2014,90(6):137.
[19]	CONTI M, HSIEH M, ZAMAH A M, et al. Novel signaling mechanisms in the ovary during oocyte maturation and ovulation[J]. Mol Cell Endocrinol,2012,356(1/2):65−73.
[20]	ADHIKARI D, LIU K. Molecular mechanisms underlying the activation of mammalian primordial follicles[J]. Endocr Rev,2009(5):438−464.
[21]	MAKKER A, GOEL M M, MAHDI A A. Pi3k/pten/akt and tsc/mtor signaling pathways, ovarian dysfunction, and infertility: An update[J]. J Mol Endocrinol,2014,53(3):103−118. doi: 10.1530/JME-14-0220
[22]	李娇娇, 焦亚飞, 石德顺. PI3K/Akt信号通路对卵泡发育和卵母细胞成熟作用的研究进展[J]. 中国畜牧杂志,2021,57(8):33−36. [LI Jiaojiao, JIAO Yafei, SHI Deshun. Research progress on the role of PI3K/Akt signaling pathway on follicle development and oocyte maturation[J]. Chinese Journal of Animal Science,2021,57(8):33−36. doi: 10.19556/j.0258-7033.20201010-02 LI Jiaojiao, JIAO Yafei, SHI Deshun. Research progress on the role of PI3K/Akt signaling pathway on follicle development and oocyte maturation[J]. Chinese Journal of Animal Science, 2021, 57(8): 33-36. doi: 10.19556/j.0258-7033.20201010-02
[23]	GROSBOIS J, DEMEESTERE I. Dynamics of pi3k and hippo signaling path ways during in vitro human follicle activation[J]. HumReprod,2018,33(9):1705−1714.
[24]	MIRZA-AGHAZADEH-ATTARI M, EKRAMI E M, AGHDAS S A M, et al. Targeting pi3k/akt/mtor signaling pathway by polyphenols: Implication for cancer therapy[J]. Life Sciences,2020(255):117481.
[25]	LEE H N, CHANG E M. Primordial follicle activation as new treatment for primary ovarian insufficiency[J]. Clin Exp Reprod Med,2019,46(2):43−49. doi: 10.5653/cerm.2019.46.2.43

[1]	MIAO Xingyu, WANG Qinghua, CHAI Chunrong, FAN Xin, REN Dabing, LI Siyu, DONG Wenjiang, HU Yongdan, YI Lunzhao. Effects of Enhanced Fermentation of Saccharomyces cerevisiae and Lactobacillus plantarum on Volatile Flavor of Coffee[J]. Science and Technology of Food Industry, 2025, 46(7): 1-10. DOI: 10.13386/j.issn1002-0306.2024050272
[2]	CUI Yan, LIU Hanxin, ZHU Lin, SHANG Haitao, LIN Xudong, CHEN Shuying, XUAN Xiaoting. Effect of High Hydrostatic Pressure Sterilization on the Physicochemical Properties, Taste, and Flavor of Large Yellow Croaker (Larimichthys crocea)[J]. Science and Technology of Food Industry, 2025, 46(5): 44-55. DOI: 10.13386/j.issn1002-0306.2024020169
[3]	LIU Yang, HUANG Jia, JIA Hongfeng, FANG Xiaowei, LONG Juyi, LAN Ning. Effects of Different Cooking Methods on Volatile Flavor Compounds in Beef[J]. Science and Technology of Food Industry, 2022, 43(10): 305-313. DOI: 10.13386/j.issn1002-0306.2021080198
[4]	LIU Guomin, QIN Weizhi, WEI Rongchang, YI Ruolan, LIAO Yujiao, ZHENG Xu, CHE Jianglü. Comparative Analysis of Volatile Flavor Compounds in Different Varieties (Lines) of Potatoes[J]. Science and Technology of Food Industry, 2022, 43(9): 284-292. DOI: 10.13386/j.issn1002-0306.2021080141
[5]	MA Guoli, TANG Shanhu, LI Sining, LIU Huilun, REN Ran. Changes of Physicochemical Properties and Volatile Flavor Substances in Tibetan Air-dried Yak Meat Jerky during the Simulated Processing[J]. Science and Technology of Food Industry, 2021, 42(2): 19-25. DOI: 10.13386/j.issn1002-0306.20200301018
[6]	YUAN Qin-qin, LIU Wen-ying. Taste and Flavor Characteristics of Peony Flower Tea under Different Soaking Methods[J]. Science and Technology of Food Industry, 2020, 41(21): 273-280. DOI: 10.13386/j.issn1002-0306.2020020195
[7]	ZHANG Kang-yi, HE Meng-ying, GUO Dong-xu, SONG Fan-fan, GAO Ling-ling, WANG Meng-xi. Effects of different drying methods on quality and volatile components of Nianzhuan[J]. Science and Technology of Food Industry, 2018, 39(2): 75-80,85. DOI: 10.13386/j.issn1002-0306.2018.02.015
[8]	LI Xing, BU Li-jun, ZHANG Xiao-chun, XIE Hua-dong, ZHONG Zheng-ze. Effects of microwave sterilization on volatile flavor compounds of spiced goose meat[J]. Science and Technology of Food Industry, 2017, (14): 97-100. DOI: 10.13386/j.issn1002-0306.2017.14.019
[9]	TANG Qiu-shi, CHEN Zhi-yi, LIU Xue-ming, YANG Chun-ying, WANG Si-yuan, LIN Yao-sheng. Influence of drying methods on volatile components of Flammulina velutipe[J]. Science and Technology of Food Industry, 2015, (10): 119-124. DOI: 10.13386/j.issn1002-0306.2015.10.016
[10]	CHEN Yi- ying, GUO Bei- bei, ZHANG Hui- ying, ZHANG Yu- yu, CHEN Hai-tao, SUN Bao-guo, XIE Jian-chun. Volatile flavor compounds analysis of the dapanji by GC- MS[J]. Science and Technology of Food Industry, 2014, (21): 291-296. DOI: 10.13386/j.issn1002-0306.2014.21.054

Supplements (0)

Cited By

Cited by

Periodical cited type(3)

1.	李颜博，杜庆萍，杨瑞，李薇，张路思，宋安康，李玮，李学文，王伟. 沙棘原浆关键加工环节品质动态变化. 江苏农业学报. 2025(02): 362-371 .
2.	陈亦辉，陈伟，尚海涛，王建成. 我国水蜜桃种质、保鲜贮运和加工技术研究进展. 浙江万里学院学报. 2024(02): 81-89 .
3.	翟若涵，张全艳，陈翠榕，高云燕. 桃果实保鲜技术研究综述. 南方农业. 2024(04): 179-181 .