Isolation, Purification, Monosaccharide Composition and Antioxidant Activity of Polysaccharides from Cortex Periplocae

Ruiping ZHANG; Zhaohui REN; Haonan ZHANG; Xiaoping ZHANG; Pengfei LIU; Xiaoli WANG

doi:10.13386/j.issn1002-0306.2022080349

Volume 44 Issue 13

Jun. 2023

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Science and Technology of Food Industry > 2023 > 44(13): 71-78. > DOI: 10.13386/j.issn1002-0306.2022080349

ZHANG Ruiping, REN Zhaohui, ZHANG Haonan, et al. Isolation, Purification, Monosaccharide Composition and Antioxidant Activity of Polysaccharides from Cortex Periplocae[J]. Science and Technology of Food Industry, 2023, 44(13): 71−78. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022080349.

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Isolation, Purification, Monosaccharide Composition and Antioxidant Activity of Polysaccharides from Cortex Periplocae

1.
College of Tobacco Science, Henan Agricultural University, Zhengzhou 450046, China
2.
Technology Center, Jilin Tobacco Industry Co., Ltd., Yanji 133001, China
3.
College of Animal Medicine, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, China

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Received Date: September 01, 2022
Available Online: May 08, 2023

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Abstract

Abstract

Objective: In this experiment, polysaccharides from cortex periplocae (CPP) were isolated and purified, and their monosaccharide composition and antioxidant activity were investigated, so as to provide a reference for the development and application of CPP in the food industry. Methods: The crude polysaccharides were obtained by aqueous extraction, alcoholic precipitation and protein removal by Sevag method, and four polysaccharide fractions CPP0, CPP1, CPP2 and CPP3 were separated and purified by DEAE-52 cellulose column. The chemical composition, molecular weight, monosaccharide composition, infrared spectrum and antioxidant activity were analyzed. Results: The sugar contents of these four polysaccharides were 82.20%, 77.13%, 75.23% and 72.85%, respectively, and all of them contained glyoxylate. The relative molecular weights were 685, 477, 411 and 572 kDa, respectively. The four polysaccharides were all heteropolysaccharides composed of mannose, rhamnose, galacturonic acid, glucose, galactose and arabinose in different molar ratios. The infrared spectra showed that four polysaccharides contained β-glycosidic bonds and with furan rings. The antioxidant experiments showed that all four polysaccharides had certain antioxidant properties, and the overall antioxidant capacity was in the following order: CPP0>CPP3>CPP1>CPP2. Conclusion: The four acidic polysaccharides extracted from cortex periplocae have high sugar content, high relative molecular weight, and antioxidant activity, among which CPP0 fraction has the best antioxidant activity.
- cortex periplocae,
- polysaccharide,
- isolation and purification,
- infrared spectroscopy,
- monosaccharide composition,
- antioxidant activity

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References

[1]	徐硕, 金鹏飞, 徐文峰, 等. 中药香加皮的研究进展[J]. 西北药学杂志,2017,32(1):118−121. [XU S, JIN P F, XU W F, et al. Advances on Chinese herbal medicine Periplocae cortex[J]. Northwest Pharmaceutical Journal,2017,32(1):118−121. doi: 10.3969/j.issn.1004-2407.2017.01.032 XU S, JIN P F, XU W F, et al. Advances on Chinese herbal medicine Periplocae cortex[J]. Northwest Pharmaceutical Journal, 2017, 32(1): 118-121. doi: 10.3969/j.issn.1004-2407.2017.01.032
[2]	李金楠, 赵丽迎, 于静, 等. 香加皮化学成分的研究[J]. 中成药,2010,32(9):1552−1556. [LI J N, ZHAO L Y, YU J, et al. Chemical constituents of the root barks of Periploca sepium[J]. Chin Tradit Pat Med,2010,32(9):1552−1556. doi: 10.3969/j.issn.1001-1528.2010.09.028 LI J N, ZHAO L Y, YU J, et al. Chemical constituents of the root barks of Periploca sepium[J]. Chin. Tradit. Pat. Med, 2010, 32: 1552-1556. doi: 10.3969/j.issn.1001-1528.2010.09.028
[3]	杨雪, 李梦雨, 颜昌钖, 等. 香加皮的化学成分与药理作用研究进展及质量标志物的预测分析[J]. 中国中药杂志,2020,45(12):2772−2783. [YANG X, LI M Y, YAN C Y, et al. Research progress on chemical composition and pharmacological effects of Periplocae cortex and predictive analysis on Q-marker[J]. China Journal of Chinese Materia Medica,2020,45(12):2772−2783. doi: 10.19540/j.cnki.cjcmm.20200327.202 YANG X, LI M Y, YAN C Y, et al. Research progress on chemical composition and pharmacological effects of Periplocae cortex and predictive analysis on Q-marker[J]. China Journal of Chinese Materia Medica, 2020, 45(12): 2772-2783. doi: 10.19540/j.cnki.cjcmm.20200327.202
[4]	CHEN S H, HUANG H L, HUANG G L. Extraction, derivatization and antioxidant activity of cucumber polysaccharide[J]. International Journal of Biological Macromolecules,2019,140:1047−1053. doi: 10.1016/j.ijbiomac.2019.08.203
[5]	WANG M, ZHU P, ZHAO S, et al. Characterization, antioxidant activity and immunomodulatory activity of polysaccharides from the swollen culms of Zizania latifolia[J]. International Journal of Biological Macromolecules,2017,95:809−817. doi: 10.1016/j.ijbiomac.2016.12.010
[6]	LIU F, MA C, MC CLEMENTS D J, et al. Development of polyphenol-protein-polysaccharide ternary complexes as emulsifiers for nutraceutical emulsions: Impact on formation, stability, and bioaccessibility of β-carotene emulsions[J]. Food Hydrocolloids,2016,61:578−588. doi: 10.1016/j.foodhyd.2016.05.031
[7]	HU B, CHEN Q, CAI Q, et al. Gelation of soybean protein and polysaccharides delays digestion[J]. Food Chemistry,2017,221:1598−1605. doi: 10.1016/j.foodchem.2016.10.132
[8]	王笑月, 刘效兰, 薛燕, 等. 黄精及其复配植物多糖提取工艺优化及人体保湿评价[J]. 食品工业科技,2018,39(5):221−225, 240. [WANG X Y, LIU X L, XUE Y, et al. Optimization of process extraction for polysaccharides of Polygonatum and its prescription and evaluation of moisture retention capacity of human body[J]. Science and Technology of Food Industry,2018,39(5):221−225, 240. doi: 10.13386/j.issn1002-0306.2018.05.042 WANG X Y, LIU X L, XUE Y, et al. Optimization of process extraction for polysaccharides of Polygonatum and its prescription and evaluation of moisture retention capacity of human body[J]. Science and Technology of Food Industry, 2018, 39(5): 221-225+240. doi: 10.13386/j.issn1002-0306.2018.05.042
[9]	KONTOGIORGOS V. Polysaccharides at fluid interfaces of food systems[J]. Advances in Colloid and Interface Science,2019,270:28−37. doi: 10.1016/j.cis.2019.05.008
[10]	BARCLAY T G, DAY C M, PETROVSKY N, et al. Review of polysaccharide particle-based functional drug delivery[J]. Carbohydrate Polymers,2019,221:94−112. doi: 10.1016/j.carbpol.2019.05.067
[11]	段漓童, 刘正猛, 劳风云, 等. 香加皮多糖分离提取及含量测定[J]. 中国煤炭工业医学杂志,2007(7):766−768. [DUAN L T, LIU Z M, LAO F Y, et al. Isolation, extraction and determination of Cortex periplocae polysaccharides[J]. Chinese Journal of Coal Industry Medicine,2007(7):766−768. doi: 10.3969/j.issn.1007-9564.2007.07.016 DUAN L T, LIU Z M, LAO F Y, et al. Isolation, extraction and determination of s Cortex periplocae polysaccharides[J]. Chinese Journal of Coal Industry Medicine, 2007(7): 766-768. doi: 10.3969/j.issn.1007-9564.2007.07.016
[12]	WANG X L, ZHANG Y F, LIU P F, et al. Purification, characterization, and antioxidant activity of polysaccharides isolated from Cortex periplocae[J]. Molecules,2017,22(11):1866. doi: 10.3390/molecules22111866
[13]	王小莉. 香加皮多糖提取纯化及其在烟草中的保润增香效应研究[D]. 郑州: 河南农业大学, 2018 WANG X L. Extraction and purification of Cortex periplocae polysaccharides and their use in tobacco moisture retention and flavor enhancement[D]. Zhengzhou: Henan Agricultural University, 2018.
[14]	曾凡珂, 李伟, 曹庸, 等. 金樱子多糖提取、纯化及生理活性研究进展[J]. 食品工业科技,2019,40(7):307−312. [ZENG F K, LI W, CAO Y, et al. Research progress on extraction, purification and physiological activities of polysaccharides from Rosa laevigata Michx[J]. Science and Technology of Food Industry,2019,40(7):307−312. doi: 10.13386/j.issn1002-0306.2019.07.053 ZENG F K, LI W, CAO Y, et al. Research progress on extraction, purification and physiological activities of polysaccharides from Rosa laevigata Michx[J]. Science and Technology of Food Industry, 2019, 40(7): 307-312. doi: 10.13386/j.issn1002-0306.2019.07.053
[15]	DUBOIS M, GILLES K A, HAMILTON J K, et al. Colorimetric method for determination of sugars and related substances[J]. Analytical Chemistry,1956,28(3):350−356. doi: 10.1021/ac60111a017
[16]	王艾平, 周丽明. 考马斯亮蓝法测定茶籽多糖中蛋白质含量条件的优化[J]. 河南农业科学,2014,43(3):150−153. [WANG A P, ZHOU L M. Determination of protein content in Camellia oleifera seed polysaccharides by Coomassie brilliant blue method[J]. Journal of Henan Agricultural Sciences,2014,43(3):150−153. doi: 10.3969/j.issn.1004-3268.2014.03.033 WANG A P, ZHOU L M. Determination of protein content in Camellia oleifera seed polysaccharides by Coomassie brilliant blue method[J]. Journal of Henan Agricultural Sciences, 2014, 43(3): 150-153. doi: 10.3969/j.issn.1004-3268.2014.03.033
[17]	任珍芸, 陈晓航, 王玺, 等. 硫酸-咔唑微孔板法检测肺炎链球菌荚膜多糖中糖醛酸含量[J]. 微生物学免疫学进展,2017,45(2):36−41. [REN Z Y, CHEN X H, WANG X, et al. Determination of uronic acid content in pneumococcal polysaccharide by sulfate-carbazole method in microplate[J]. Progress in Microbiology and Immunology,2017,45(2):36−41. REN Z Y, CHEN X H, WANG X, et al Determination of uronic acid content in pneumococcal polysaccharide by sulfate-carbazole method in microplate[J]. Progress in Microbiology and Immunology, 2017, 45(2): 36-41.
[18]	王少杰, 巴娟, 张勇军, 等. 白术多糖的分子量及其单糖组成分析[J]. 中国兽药杂志,2019,53(5):26−32. [WANG S J, BA J, ZHANG Y J, et al. Analysis of molecular weight and monosaccharide composition of Atractylodes macrocephala polysaccharides[J]. Chinese Journal of Veterinary Drug,2019,53(5):26−32. WANG S J, BA J, ZHANG Y J, et al. Analysis of molecular weight and monosaccharide composition of Atractylodes macrocephala polysaccharides[J]. Chinese Journal of Veterinary Drug, 2019, 53(5): 26-32.
[19]	余亦婷, 皮文霞, 谢辉, 等. PMP柱前衍生化-HPLC法分析不同生长年限黄芪中6种单糖的含量[J]. 中国药房,2021,32(12):1448−1452. [YU Y T, PI W X, XIE H, et al. Content determination of 6 kinds of monosaccharide in Astragalus membranaceus with different growth years by pre-column derivatization-HPLC[J]. China Pharmacy,2021,32(12):1448−1452. doi: 10.6039/j.issn.1001-0408.2021.12.07 YU Y T, PI W X, XIE H, et al. Content determination of 6 kinds of monosaccharide in Astragalus membranaceus with different growth years by pre-column derivatization-HPLC[J]. China Pharmacy, 2021, 32(12): 1448-1452. doi: 10.6039/j.issn.1001-0408.2021.12.07
[20]	SHEN S, JIA S, WU Y, et al. Effect of culture conditions on the physicochemical properties and antioxidant activities of polysaccharides from Nostoc flagelliforme[J]. Carbohydrate Polymers,2018,198:426−433. doi: 10.1016/j.carbpol.2018.06.111
[21]	程爽, 贺斐, 付龙洋, 等. 冬凌草硒多糖的制备及其抗氧化活性分析[J]. 精细化工,2021,38(10):2064−2071. [CHENG S, HE P, FU L Y, et al. Preparation of selenium polysaccharide from Rabdosia rubescens and analysis of its antioxidant activity[J]. Fine Chemicals,2021,38(10):2064−2071. doi: 10.13550/j.jxhg.20210320 CHENG S, HE P, FU L Y, et al. Preparation of selenium polysaccharide from Rabdosia rubescens and analysis of its antioxidant activity[J]. Fine Chemicals, 2021, 38(10): 2064-2071. doi: 10.13550/j.jxhg.20210320
[22]	宋丽丽, 闻格, 霍姗浩, 等. 小黄姜多糖的分离纯化及其结构特征及抗氧化活性研究[J]. 食品与发酵工业,2020,46(12):73−79. [SONG L L, WEN G, HUO S H, et al. Isolation, purification, structural characterization and antioxidant activity of polysaccharide from Zingiber officinale Roscoe[J]. Food and Fermentation Industries,2020,46(12):73−79. doi: 10.13995/j.cnki.11-1802/ts.023519 SONG L L, WEN G, HUO S H, et al. Isolation, purification, structural characterization and antioxidant activity of polysaccharide from Zingiber officinale Roscoe[J]. Food and Fermentation Industries, 2020, 46(12): 73-79. doi: 10.13995/j.cnki.11-1802/ts.023519
[23]	徐雯雯, 陆春霞, 肖潇, 等. 桑黄多糖的提取纯化技术及药理作用研究进展[J]. 蚕学通讯,2022,42(3):20−30. [XU W W, LU C X, XIAO X, et al. Research progress on the extraction and purification technology and pharmacological effects of polysaccharides from Sanghuangporus[J]. Newsletter of Sericultural Science,2022,42(3):20−30. doi: 10.3969/j.issn.1006-0561.2022.03.005 XU W W, LU C X, XIAO X, et al. Research progress on the extraction and purification technology and pharmacological effects of polysaccharides from Sanghuangporus [J]. Newsletter of Sericultural Science, 2022, 42(3): 20-30. doi: 10.3969/j.issn.1006-0561.2022.03.005
[24]	闫征, 郝利民, 张黎明, 等. 3种不同产地灵芝子实体主要化学成分比较[J]. 食品科学,2019,40(6):240−246. [YAN Z, HAO L M, ZHANG L M, et al. Comparison of main chemical constituents in Ganoderma lucidum fruiting bodies collected from three producing regions[J]. Food Science,2019,40(6):240−246. doi: 10.7506/spkx1002-6630-20180620-396 YAN Z, HAO L M, ZHANG L M, et al. Comparison of main chemical constituents in Ganoderma lucidum fruiting bodies collected from three producing regions[J]. Food Science, 2019, 40(6): 240-246. doi: 10.7506/spkx1002-6630-20180620-396
[25]	WANG L, LIU H M, Xie A J, et al. Chinese quince (Chaenomeles sinensis) seed gum: Structural characterization[J]. Food Hydrocolloids,2018,75:237−245. doi: 10.1016/j.foodhyd.2017.08.001
[26]	艾于杰. 抗氧化活性茶多糖构效关系研究[D]. 武汉: 华中农业大学, 2019 AI Y J. Study on the conformational relationship of antioxidant active tea polysaccharides[D]. Wuhan: Huazhong Agricultural University, 2019.
[27]	LI F, GAO J, XUE F, et al. Extraction optimization, purification and physicochemical properties of polysaccharides from Gynura medica[J]. Molecules,2016,21(4):397. doi: 10.3390/molecules21040397
[28]	XU Z, LI X, FENG S, et al. Characteristics and bioactivities of different molecular weight polysaccharides from Camellia seed cake[J]. International Journal of Biological Macromolecules,2016,91:1025−1032. doi: 10.1016/j.ijbiomac.2016.06.067
[29]	胡彦波, 翟丽媛, 刘扬, 等. 薇菜多糖的分离纯化及体外抗氧化活性[J]. 食品科学,2022,43(1):59−66. [HU Y B, ZHAI L Y, LIU Y, et al. Isolation, purification and antioxidant activity of polysaccharides from Osmunda japonica[J]. Food Science,2022,43(1):59−66. doi: 10.7506/spkx1002-6630-20210426-373 HU Y B, ZHAI L Y, LIU Y, et al. Isolation, purification and antioxidant activity of polysaccharides from Osmunda japonica[J]. Food Science, 2022, 43(1): 59-66. doi: 10.7506/spkx1002-6630-20210426-373
[30]	凌洁玉, 周文轩, 庄志雄, 等. 鲊辣椒多糖组分变化初步分析及抗氧化活性研究[J]. 东北农业科学,2022,47(3):145−149. [LING J Y, ZHOU W X, ZHUANG Z X, et al. Analysis of components and antioxidant activity of Fermented cayenne polysaccharides[J]. Journal of Northeast Agricultural Sciences,2022,47(3):145−149. LING J Y, ZHOU W X, ZHUANG Z X, et al. Analysis of components and antioxidant activity of Fermented cayenne polysaccharides[J]. Journal of Northeast Agricultural Sciences, 2022, 47(3): 145-149.
[31]	伍晓萍, 代玉玲, 张玲, 等. 三七多糖吸湿、保湿性能及体外抗氧化活性[J]. 昆明医科大学学报,2022,43(5):1−6. [WU X P, DAI Y L, ZHANG L, et al. Studies on moisture-absorption-retention and antioxidant activity of Panax notoginseng polysaccharides in vitro[J]. Journal of Kunming Medical University,2022,43(5):1−6. doi: 10.12259/j.issn.2095-610X.S20220514 WU X P, DAI Y L, ZHANG L, et al. Studies on moisture-absorption-retention and antioxidant activity of Panax notoginseng polysaccharides in vitro[J]. Journal of Kunming Medical University, 2022, 43(5): 1-6. doi: 10.12259/j.issn.2095-610X.S20220514
[32]	孙明杰, 张越, 姚亮, 等. 茯苓多糖的分离纯化、组成及其抗氧化活性研究[J]. 安徽中医药大学学报,2022,41(1):86−91. [SUN M J, ZHANG Y, YAO L, et al. Isolation, purification, composition, and antioxidant activity of Poria cocos polysaccharides[J]. Journal of Anhui University of Chinese Medicine,2022,41(1):86−91. doi: 10.3969/j.issn.2095-7246.2022.01.017 SUN M J, ZHANG Y, YAO L, et al. Isolation, purification, composition, and antioxidant activity of Poria cocos polysaccharides[J]. Journal of Anhui University of Chinese Medicine, 2022, 41(1): 86-91. doi: 10.3969/j.issn.2095-7246.2022.01.017
[33]	董扬, 郝利民, 刘宇琪, 等. 不同破壁方法对灵芝孢子粗多糖抗氧化活性的影响[J]. 食品科学,2017(17):101−106. [DONG Y, HAO L M, LIU Y Q, et al. Extracts and biological activities of Sporodem-broken spores of Ganoderma lucidum[J]. Food Science,2017(17):101−106. doi: 10.7506/spkx1002-6630-201717017 DONG Y, HAO L M, LIU Y Q, et al. Extracts and biological activities of Sporodem-broken spores of Ganoderma lucidum[J]. Food Science, 2017(17): 101-106. doi: 10.7506/spkx1002-6630-201717017
[34]	冯湘沅, 成莉凤, 谢纯良, 等. 不同品种黄麻叶多糖的理化特征及抗氧化活性研究[J]. 天然产物研究与开发,2020,32(8):1278−1284. [FENG X Y, CHENG L F, XIE C L, et al. Study on the physicochemical characteristics and antioxidant activities of polysaccharides from different varieties of jute leaves[J]. Natural Product Research and Development,2020,32(8):1278−1284. doi: 10.16333/j.1001-6880.2020.8.002 FENG X Y, CHENG L F, XIE C L, et al. Study on the physicochemical characteristics and antioxidant activities of polysaccharides from different varieties of jute leaves[J]. Natural Product Research and Development, 2020, 32(8): 1278-1284. doi: 10.16333/j.1001-6880.2020.8.002

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