Preparation, Structural Characterization and <i>in Vitro</i> Activity of Chromium Complexes of Sweet Corn Cob Polysaccharide

Weiye XIU; Xin WANG; Yumeng LI; Shiyou YU; Chenchen LI; Yu LUO; Zhuo ZHOU; Yongqiang MA

doi:10.13386/j.issn1002-0306.2022080338

Volume 44 Issue 13

Jun. 2023

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Science and Technology of Food Industry > 2023 > 44(13): 186-196. > DOI: 10.13386/j.issn1002-0306.2022080338

XIU Weiye, WANG Xin, LI Yumeng, et al. Preparation, Structural Characterization and in Vitro Activity of Chromium Complexes of Sweet Corn Cob Polysaccharide[J]. Science and Technology of Food Industry, 2023, 44(13): 186−196. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022080338.

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Preparation, Structural Characterization and in Vitro Activity of Chromium Complexes of Sweet Corn Cob Polysaccharide

Key Laboratory of Cereal Food and Cereal Resources in Heilongjiang Province, School of Food Engineering, Harbin University of Commerce, Harbin 150028, China

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Received Date: August 30, 2022
Available Online: May 07, 2023

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Abstract

Abstract

To enhance the biological activity of sweet corn cob polysaccharide, a sweet corn cob polysaccharide chromium complex (SCP80-Cr) was prepared, characterized and its in vitro activity was also explored. The results showed that the optimal process conditions for the preparation were pH10.1, reaction temperature 74 ℃, polysaccharide concentration of 3 mg/mL, and the reaction time of 60 min. The weight percentage of Cr(III) in SCP80-Cr was 21.08% and the elemental percentage was 6.67%. The characterization revealed that SCP80-Cr contained almost no protein, nucleic acid or pigment, while Cr was conjugated with SCP80 in the form of Cr-O and Cr-OH and had strong molecular agglomeration properties. The molar percentage of the monosaccharide composition of SCP80-Cr was mannose: glucose: galactose: xylose: arabinose = 0.58: 89.50: 4.65: 1.33: 3.58. The results of in vitro hypoglycemia experiments showed that the IC₅₀ of SCP80-Cr on α-amylase and α-glucosidase inhibitions were 4.70±0.38 mg/mL and 3.99±0.26 mg/mL, respectively. Moreover, the results of in vitro antioxidant experiments showed that SCP80-Cr scavenged DPPH radicals and hydroxyl radicals with IC₅₀ of 4.80±0.34 mg/mL and 4.99±0.28 mg/mL, respectively, indicating that SCP80-Cr had better in vitro hypoglycemic and antioxidant activities compared with SCP80. The results of the study provided some references for the study of sweet corn cob polysaccharides in functional foods.

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[1]	BI Z, ZHAO Y, HU J, et al. A novel polysaccharide from Lonicerae japonicae caulis: Characterization and effects on the function of fibroblast-like synoviocytes[J]. Carbohydrate Polymers,2022,292:119674. doi: 10.1016/j.carbpol.2022.119674
[2]	WANG S, LI G, ZHANG X, et al. Structural characterization and antioxidant activity of Polygonatum sibiricum polysaccharides[J]. Carbohydrate Polymers,2022,291:119524. doi: 10.1016/j.carbpol.2022.119524
[3]	SONG J, WU Y, MA X, et al. Structural characterization and α-glycosidase inhibitory activity of a novel polysaccharide fraction from Aconitum coreanum[J]. Carbohydrate Polymers,2020,230(C):115586.
[4]	LIU W, HU C, LIU Y, et al. Preparation, characterization, and α-glycosidase inhibition activity of a carboxymethylated polysaccharide from the residue of Sarcandra glabra (Thunb.) Nakai[J]. International Journal of Biological Macromolecules, 2017, 99: 454−464.
[5]	LU K, CHEN S, LIN Y, et al. An antidiabetic nutraceutical combination of red yeast rice (Monascus purpureus), bitter gourd (Momordica charantia), and chromium alleviates dedifferentiation of pancreatic β cells in db/db mice[J]. Food Science & Nutrition, 2020, 8(12): 6718-6726.
[6]	YE H, SHEN Z, CUI J, et al. Hypoglycemic activity and mechanism of the sulfated rhamnose polysaccharides chromium(III) complex in type 2 diabetic mice[J]. Bioorganic Chemistry, 2019, 88: 102942.
[7]	WANG X, XIU W, HAN Y, et al. Structural characterization of a novel polysaccharide from sweet corncob that inhibits glycosylase in STZ-induced diabetic rats: Structural characterization of a novel polysaccharide[J]. Glycoconjugate Journal,2022,39(3):413−427.
[8]	WANG X, WANG Z, ZHANG K, et al. Effects of sweet corncob polysaccharide on pancreatic protein expression in type 2 diabetic rats[J]. Journal of Functional Foods, 2022, 88.
[9]	MA Y, WANG X, GAO S. Hypoglycemic activity of polysaccharides from sweet corncob on streptozotocin-induced diabetic rats.[J]. Journal of Food Science,2017,82(1):208−213. doi: 10.1111/1750-3841.13554
[10]	WANG X, XIU W, HAN Y. et al. Structural characterization of a non-starch polysaccharide from sweet corn cobs[J]. Journal of Food Processing and Preservation, 2022, 46(9).
[11]	秦月. 虎眼万年青多糖及其铬复合物降血糖作用的研究[D]. 哈尔滨: 黑龙江中医药大学, 2018: 30−32 QIN Y. Hypoglycemic effect of Ornithogalum caudatum polysaccharide and its chromium complex[D]. Harbin: Heilongjiang University of Chinese Medicine, 2018: 30−32.
[12]	公衍玲, 郭遥遥, 刘洋. 樱桃核乙醇提取物体外抗氧化及降糖降脂研究[J]. 青岛科技大学学报(自然科学版),2017,38(6):14−17. [GONG Y L, GUO Y Y, LIU Y. Study on in vitro antioxidant, hypoglycemic and lipid-lowering activities of cherry kernel ethanol extracts[J]. Journal of Qingdao University of Science and Technology(Natural Science Edition),2017,38(6):14−17. GONG Y, GUO Y, LIU Y. Study on in vitro antioxidant, hypoglycemic and lipid-lowering activities of cherry kernel ethanol extracts[J]. Journal of Qingdao University of Science and Technology(Natural Science Edition), 2017, 38(6): 14-17.
[13]	陈玥彤, 张闪闪, 李文意, 等. 黑木耳多糖的磷酸化修饰、结构表征及体外降糖活性[J]. 食品科学,2022,43(8):29−35. [CHEN Y T, ZHANG S S, LI W Y, et al. Structural characterization and hypoglycemic effect in vitro of phosphorylated Auricularia auriculata polysaccharide[J]. Food Science,2022,43(8):29−35. CHEN Y, ZHANG S, LI W, et al. Structural characterization and hypoglycemic effect in vitro of phosphorylated Auricularia auriculata polysaccharide[J] Food Science, 2022, 43(8): 29-35.
[14]	李春英, 夏依拉·艾尼, 毛建卫, 等. 栀子黄对α-葡萄糖苷酶抑制活性的研究[J]. 食品工业科技,2015,36(24):112−114, 119. [LI C Y, XIAYILA A, MAO J W, et al. Study on the inhibitory effect of gardenia yellow on α-glucosidase activity[J]. Science and Technology of Food Industry,2015,36(24):112−114, 119. LI C, XIAYILA A, MAO, J, et al. Study on the inhibitory effect of gardenia yellow on α-glucosidase activity[J] Science and Technology of Food Industry, 2015, 36(24): 112-114, 119.
[15]	ZUO M, LIU X, LIU D, et al. Extraction, Characterization and antioxidant activity in vitro of proteins from Semen Allii Fistulosi[J]. Molecules,2018,23(12):3235. doi: 10.3390/molecules23123235
[16]	ZHANG Y, CHEN Z, HUANG Z, et al. A comparative study on the structures of Grifola Frondosa polysaccharides obtained by different decolourization methods and their in vitro antioxidant activities[J]. Food & Function,2019,10(10):.6720−6731.
[17]	LI L, XU J, CAO Y, et al. Preparation of Ganoderma lucidum polysaccharide-chromium (III) complex and its hypoglycemic and hypolipidemic activities in high-fat and high-fructose diet-induced prediabetic mice[J]. International Journal of Biological Macromolecules,2019,140(C):782−793.
[18]	QIU J, ZHANG H, WANG Z, et al. Response surface methodology for the synthesis of an Auricularia auricula-judae polysaccharides CDDP complex[J]. International Journal of Biological Macromolecules,2016,93:333−343. doi: 10.1016/j.ijbiomac.2016.06.066
[19]	GUO W, SHI F, LI L, et al. Preparation of a novel Grifola frondosa polysaccharide-chromium (III) complex and its hypoglycemic and hypolipidemic activities in high fat diet and streptozotocin-induced diabetic mice[J]. International Journal of Biological Macromolecules,2019,131:81−88. doi: 10.1016/j.ijbiomac.2019.03.042
[20]	张雯, 张禧庆, 董淑君, 等. 南瓜皮多糖铬的制备与体外消化的分析[J]. 食品工业科技,2022,43(17):223−229. [ZHANG W, ZHANG X Q, DONG S J, et al. Preparation and in vitro digestibility of pumpkin peel polysaccharide chromium(III) complex[J]. Science and Technology of Food Industry,2022,43(17):223−229. doi: 10.13386/j.issn1002-0306.2021110329 ZHANG W, ZHANG X, DONG S, et al. Preparation and in vitro digestibility of pumpkin peel polysaccharide chromium(III) complex[J]. Science and Technology of Food Industry, 2022, 43(17): 223-229. doi: 10.13386/j.issn1002-0306.2021110329
[21]	邰克东, 赵苏茂, 杨紫恒, 等. 高压均质对脂质体囊泡特性和稳定性的影响[J]. 食品科学,2019,40(17):169−177. [TAI K D, ZHAO S M, YANG Z H. et al. Effect of high-pressure homogenization on vesicle characteristics and stability of liposomes[J]. Food Science,2019,40(17):169−177. doi: 10.7506/spkx1002-6630-20180916-158 TAI K, ZHAO S, YANG Z. et al. Effect of high-pressure homogenization on vesicle characteristics and stability of liposomes[J]. Food Science, 2019, 40(17): 169-177. doi: 10.7506/spkx1002-6630-20180916-158
[22]	郑成娟, 李珊珊, 陈青. 金樱子果渣中脂肪酸与玉米淀粉包合物的制备及性能[J]. 精细化工,2020,37(12):2482−2489. [ZHENG C J, LI S S, CHEN Q. Preparation and properties of inclusion compound of corn starch and fatty acid in Rasa laevigata pomace[J]. Fine Chemicals,2020,37(12):2482−2489. doi: 10.13550/j.jxhg.20200656 ZHENG C, LI S, CHEN Q. Preparation and properties of inclusion compound of corn starch and fatty acid in Rasa laevigata pomace[J]. Fine Chemicals, 2020, 37(12): 2482-2489. doi: 10.13550/j.jxhg.20200656
[23]	张秀娟, 刘治廷, 杨诗涵, 等. 超声波辅助酶法提取蓝莓果渣花色苷的工艺优化及降解动力学[J]. 精细化工,2022,39(10):2069−2077, 2098. [ZHANG X J, LIU Z T, YANG S H, et al. Condition optimization and degradation kinetics of anthocyanins from blueberry pomace by ultrasonic-assisted enzymatic extraction[J]. Fine Chemicals,2022,39(10):2069−2077, 2098. ZHANG X, LIU Z, YANG S, et al. Condition optimization and degradation kinetics of anthocyanins from blueberry pomace by ultrasonic-assisted enzymatic extraction[J], Fine Chemicals, 2022, 39(10): 2069-2077, 2098.
[24]	ZHANG W, LI L, MA Y, et al. Structural characterization and hypoglycemic activity of a novel pumpkin peel polysaccharide-chromium(III) complex[J]. Foods, 2022, 11(13): 1821-1821.
[25]	全志刚, 王维浩, 赵姝婷, 等. 小米麸皮水溶性膳食纤维-Cr(III)配合物的合成、表征及其体外抗氧化活性[J]. 食品科学,2021,42(21):46−55. [QUAN Z G, WANG W H, ZHAO S T, et al. Synthesis, characterization and antioxidant activity of millet bran soluble dietary fiber-Cr(Ⅲ) complexes[J]. Food Science,2021,42(21):46−55. doi: 10.7506/spkx1002-6630-20200902-023 QUAN Z, WANG W, ZHAO S, et al. Synthesis, characterization and antioxidant activity of millet bran soluble dietary fiber-Cr(Ⅲ) complexes[J]. Food Science, 2021, 42(21): 46-55. doi: 10.7506/spkx1002-6630-20200902-023
[26]	TAO E, CHENG Y, YANG S, et al. Recovering Cr(III) from chromium-containing waste: An in-depth study on mechanism via retaining organic matters[J]. Journal of Environmental Chemical Engineering, 2021, 9(5).
[27]	刘思美, 赵鹏, 张婷婷, 等. 地黄硒多糖的合成、表征及免疫活性分析[J]. 中国中药杂志,2022,47(11):2938−2946. [LIU S M, ZHAO P, ZHANG T T, et al. Synthesis, characterization, and immunological activity of Rehmannia glutinosa seleno-polysaccharides[J]. China Journal of Chinese Materia Medica,2022,47(11):2938−2946. doi: 10.19540/j.cnki.cjcmm.20220224.304 LIU S, ZHAO P, ZHANG T, et al. Synthesis, characterization, and immunological activity of Rehmannia glutinosa seleno-polysaccharides[J]. China Journal of Chinese Materia Medica, 2022, 47(11): 2938-2946. doi: 10.19540/j.cnki.cjcmm.20220224.304
[28]	汤陈鹏, 吕峰, 王蓉琳. 孔石莼多糖锌结构表征与体外降血糖活性[J]. 食品科学,2020,41(7):52−58. [TANG C P, LÜ F, WANG R L. Structural characterization and hypoglycemic activity in vitro of Ulva pertusa polysaccharides-zinc complex[J]. Food Science,2020,41(7):52−58. doi: 10.7506/spkx1002-6630-20190320-263 TANG C, LV F, WANG R. Structural characterization and hypoglycemic activity in vitro of Ulva pertusa polysaccharides-zinc complex[J]. Food Science, 2020, 41(7): 52-58. doi: 10.7506/spkx1002-6630-20190320-263
[29]	WANG C, GAO X, SANTHANAM R, et al. Effects of polysaccharides from Inonotus obliquus and its chromium (III) complex on advanced glycation end-products formation, α-amylase, α-glucosidase activity and H₂O₂ -induced oxidative damage in hepatic L02 cells[J]. Food and Chemical Toxicology, 2018, 116(Pt B): 335−345.
[30]	ZHANG J, CHEN C, FU X. et al. Fructus mori L. polysaccharide-iron chelates formed by self-embedding with iron(iii) as the core exhibit good antioxidant activity[J]. Food & Function,2019,10(6):3150−3160.

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