Improving Effects of Epigallocatechin-3-gallate (EGCG) on Dextran Sulfate Sodium (DSS)-Induced Colitis

Jie LI; Zhixu ZHANG

doi:10.13386/j.issn1002-0306.2022080331

Volume 44 Issue 13

Jun. 2023

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Science and Technology of Food Industry > 2023 > 44(13): 390-397. > DOI: 10.13386/j.issn1002-0306.2022080331

LI Jie, ZHANG Zhixu. Improving Effects of Epigallocatechin-3-gallate (EGCG) on Dextran Sulfate Sodium (DSS)-Induced Colitis[J]. Science and Technology of Food Industry, 2023, 44(13): 390−397. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022080331.

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Improving Effects of Epigallocatechin-3-gallate (EGCG) on Dextran Sulfate Sodium (DSS)-Induced Colitis

Jie LI^1,,
Zhixu ZHANG^{2, 3, 4, ,}

1.
College of Food Science and Technology, Hunan Agricultural University, Changsha 410128 , China
2.
Horticulture College, Hunan Agricultural University, Changsha 410128, China
3.
State Key Laboratory of Subhealth Intervention Technology, Changsha 410128, China
4.
Hunan Co-Innovation Center for Utilization of Botanical Functional Ingredients, Changsha 410128, China

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Received Date: August 30, 2022
Available Online: May 08, 2023

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Abstract

Abstract

This study aimed to investigate the effect of epigallocatechin-3-gallate (EGCG) on dextran sulfate sodium (DSS)-induced colitis and gut microbiota in mice. The C57BL/6 male mice were divided into normal control group, enteritis model group and EGCG treatment group (50 mg/kg), 10 mice in each group, and were administered orally for 9 consecutive days. The improvement effect of EGCG on DSS-induced colitis in mice was evaluated by weighing the weight of the mice, observing and recording the fecal viscosity and fecal bleeding of the mice, measuring the length of the colon in the mice and detecting inflammatory factors in the serum. The effect of EGCG on the gut microbiota of DSS-induced colitis mice was evaluated by analyzing colon pathological morphology, expression of tight junction proteins, the diversity and structure of gut microbiota. The results showed that EGCG effectively improved the adverse reactions in DSS-induced colitis mice such as weight loss, diarrhea, blood in the stool, and colon, and alleviated systemic chronic inflammation and intestinal barrier damage in DSS-induced colitis mice. Furthermore, EGCG also ameliorated the gut microbiota disturbance, restored gut microbiota diversity, decreased the relative abundance of Firmicutes, increased the relative abundance of Bacteroidetes, promoted the proliferation of beneficial bacteria such as Akkermansia, Alistipes and Bacteroides, and inhibited the growth of harmful bacteria including Desulfovibrio, Escherichia-Shigella and Helicobacter. Therefore, EGCG effectively ameliorated DSS-induced colitis in mice by protecting the intestinal barrier and regulating gut microbiota disturbance.
- epigallocatechin-3-gallate,
- dextran sulfate sodium,
- colitis,
- gut microbiota

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References

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