Assisted Extraction Combined with Effervescent Tablets and Rhodamine B Fluorescence Probe for Rapid Detection of Elemental Mercury in Grain

Jinglei ZHANG; Liangang ZHANG; Lieqin NIU; Xinkui ZHOU; Haibo SHEN; Jianmin LI; Jianru FANG

doi:10.13386/j.issn1002-0306.2022080136

Volume 44 Issue 13

Jun. 2023

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2023 > 44(13): 325-331. > DOI: 10.13386/j.issn1002-0306.2022080136

ZHANG Jinglei, ZHANG Liangang, NIU Lieqin, et al. Assisted Extraction Combined with Effervescent Tablets and Rhodamine B Fluorescence Probe for Rapid Detection of Elemental Mercury in Grain[J]. Science and Technology of Food Industry, 2023, 44(13): 325−331. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022080136.

Citation:

PDF (1465 KB)

Assisted Extraction Combined with Effervescent Tablets and Rhodamine B Fluorescence Probe for Rapid Detection of Elemental Mercury in Grain

1.
Jiayuguan City, Gansu Province Food Medicine and Medical Equipment Inspection and Testing Center, Jiayuguan 735100, China
2.
Institute for Disease Control and Prevention, China Railway Lanzhou Bureau Group Co., Ltd., Jiayuguan 735100, China
3.
Gansu Zhongshang Food Quality Test and Detection Co., Ltd., Lanzhou 730010, China
4.
Zhangye Pharmaceutical Inspection and Testing Center, Zhangye 734000, China

More Information

Received Date: August 14, 2022
Available Online: May 03, 2023

Graphical Abstract

Abstract

Abstract

To establish a rapid method for determination of mercury in grain, Rhodamine type B fluorescent probe was synthesized and used as the carrier and chromogenic group of the probe system. The original fluorescent probe system was made into a visible light probe system, where this principle was used in conjunction with extraction assisted by effervescent tablets to establish a rapid method for the detection of elemental mercury (Hg) in grain. The initial speed of the blast reaction was controlled via the use of the bubble tablet blast method and the loss of Hg could be reduced in the course of the initial blast. After combining with different concentrations of Hg, the probe system make the originally colorless probe system turn into a rosy red color. The color developing probe system was in the range of 0.5~20 ng/g of Hg content in the sample at 550 nm, and the shade of color was proportional to the Hg content, whose range in the samples could also be assessed using a colorimetric card with naked eye. In the present study, the recoveries were 90.98%~102.46%, the precision RSD was 2.76%~4.76% and the detection limit was 1.0 ng/g. The rapid detection method has the advantages of high efficiency, accuracy, high sensitivity, specificity and wide applicability. Taken together, it is suitable for bulk screening and detection of elemental mercury in grains, wheat and other grains and their food products. Also, the method and principle might provide novel insights for the field of visualization probe rapid detection.
- elemental mercury,
- grain,
- rapid detection,
- functional ionic liquids,
- visible light probe system,
- effervescent tablets

FullText(HTML)

References (26)

References

[1]	冯新斌, 史建波, 李平, 等. 我国汞污染研究与履约进展[J]. 中国科学院院刊,2020,35(11):1344−1348. [FENG X B, SHI J B, LI P, et al. Research and performance of mercury pollution in China[J]. Bulletin of Chinese Academy of Sciences,2020,35(11):1344−1348. FENG X B, SHI J B, LI P, et al. Research and performance of mercury pollution in China[J]. Bulletin of Chinese Academy of Sciences, 2020, 35(11): 1344-1348.
[2]	吴丽珠, 高红梅, 马英. 上海市青浦区主要市售谷物及其制品的铅, 镉, 汞污染及健康风险评估[J]. 上海预防医学,2019,31(6):451−456. [WU L Z, GAO H M, MA Y. Lead, cadmium and mercury pollution and health risk assessment for main marketed grain and its products in Qingpu District of Shanghai[J]. Shanghai Journal of Preventive Medicine,2019,31(6):451−456. WU L Z, GAO H M, MA Y. Lead, cadmium and mercury pollution and health risk assessment for main marketed grain and its products in Qingpu District of Shanghai[J]. Shanghai Journal of Preventive Medicine, 2019, 31(6): 451-456.
[3]	王艳敏, 周鸿, 熊丽, 等. 江西省蔬菜中重金属污染状况调查及评价[J]. 现代预防医学,2020,47(7):1202−1206. [WANG Y M, ZHOU H, XIONG L, et al. Heavy metal pollution in vegetables, Jiangxi Province[J]. Modern Preventive Medicine,2020,47(7):1202−1206. WANG Y M, ZHOU H, XIONG L, et al. Heavy metal pollution in vegetables, Jiangxi Province[J]. Modern Preventive Medicine, 2020, 47(7): 1202-1206.
[4]	法焕宝, 闫兴菊, 尹伟, 等. 单羟基卟羟啉分光光度法测定金属铅, 汞离子[J]. 广东化工,2010,38(1):154−156. [FA H B, YAN X J, YI W, et al. Determination of metallic lead and mercury ions by mono-hydroxyporphyrin spectrophotometry[J]. Guangdong Chemical Industry,2010,38(1):154−156. FA H B, YAN X J, YI W, et al. Determination of metallic lead and mercury ions by mono-hydroxyporphyrin spectrophotometry[J]. Guangdong Chemical Industry, 2010, 38(1): 154-156.
[5]	张夏红, 董雁, 荀瑞芝, 等. 1-(4-二甲氨基苯亚甲基氨基)-4-甲基硫脲的合成及荧光猝灭法测定海产品中的微量汞[J]. 化学研究与应用,2020,32(10):1839−1846. [ZHANG X H, DONG Y, XUN R Z, et al. Synthesis of 1-(4-dimethyl aminobenzylidene) -4-methyl-thiourea and flucenorese quenching determination of trace mercury in sea products[J]. Chemistry Research and Application,2020,32(10):1839−1846. ZHANG X H, DONG Y, XUN R Z, et al. Synthesis of 1-(4-dimethyl aminobenzylidene) -4-methyl-thiourea and flucenorese quenching determination of trace mercury in sea products[J]. Chemistry Research and Application, 2020, 32(10): 1839-1846.
[6]	操江飞, 韦寿莲, 廖根成. 纳米银溶胶催化氧化-分光光度法快速测定水中汞[J]. 理化检验:化学分册,2020,56(7):816−821. [CAO J F, WEI S L, LIAO G C. Rapid Determination of mercury in water by catalytic oxidation of nanometer silver sol and spectrophotometry[J]. Physical Testing and Chemical Analysis(Part B:Chemical Analysis),2020,56(7):816−821. CAO J F, WEI S L, LIAO G C. Rapid Determination of mercury in water by catalytic oxidation of nanometer silver sol and spectrophotometry[J]. Physical Testing and Chemical Analysis(Part B: Chemical Analysis), 2020, 56(7): 816-821.
[7]	贺爱珍. 吹扫捕集-气相色谱-冷原子荧光光谱法测定水中烷基汞的方法研究[J]. 科学技术创新,2021(21):40−42. [HE A Z. Determination of alkylmercury in water by purge and capture gas chromatography-cold atomic fluorescence spectrometry[J]. Scientific and Technological Innovation,2021(21):40−42. doi: 10.3969/j.issn.1673-1328.2021.21.019 HE A Z. Determination of alkylmercury in water by purge and capture gas chromatography-cold atomic fluorescence spectrometry[J]. Scientific and Technological Innovation, 2021(21): 40-42. doi: 10.3969/j.issn.1673-1328.2021.21.019
[8]	郑明, 廖红卫, 吴俊慧, 等. ICP-MS法测定马来酸艾维替尼中钯, 砷, 镉, 汞, 铅的残留量[J]. 药物分析杂志,2020,40(5):837−842. [ZHENG M, LIAO H W, WU J H, et al. Determination of Pd, As, Cd, Hg and Pb residue in avitinib maleate by ICP-MS[J]. Chin J Pharm Anal,2020,40(5):837−842. ZHENG M, LIAO H W, WU J H, et al. Determination of Pd, As, Cd, Hg and Pb residue in avitinib maleate by ICP-MS[J]. Chin J Pharm Anal, 2020, 40(5): 837-842.
[9]	张春华, 陈春桃, 韩必恺, 等. ICP-MS测定5种中药注射液中重金属及有害元素[J]. 中国测试,2020,46(1):71−76. [ZHANG C H, CHEN C T, HAN B K, et al. Determination of heavy metals and harmful elements in five traditional Chinese medicine injections using inductively coupled plasma mass spectrometry (ICP-MS)[J]. China Measurement & Test,2020,46(1):71−76. ZHANG C H, CHEN C T, HAN B K, et al. Determination of heavy metals and harmful elements in five traditional Chinese medicine injections using inductively coupled plasma mass spectrometry (ICP-MS)[J]. China Measurement & Test, 2020, 46(1): 71-76.
[10]	徐迪伟, 陈荣乐. ICP-MS检测饮用水中31种元素的方法研究[J]. 中国卫生检验杂志,2017,27(19):2761−2764. [XU D W, CHEN R L. Study on the determination of 31 metal elements in drinking water by inductively coupled plasma mass spectrometry[J]. Chin J Health Lab Tec,2017,27(19):2761−2764. XU D W, CHEN R L. Study on the determination of 31 metal elements in drinking water by inductively coupled plasma mass spectrometry[J]. Chin J Health Lab Tec, 2017, 27(19): 2761-2764.
[11]	杜庆鹏, 陈安珍, 田金改, 等. 中药材中重金属汞快速检测试纸方法研究[J]. 中国药事,2010,24(12):1175−1177. [DU Q P, CHEN A Z, TIAN J G, et al. Study on method for rapid determination of mercury in traditional Chinese medicines[J]. Chinese Pharmaceutical Affairs,2010,24(12):1175−1177. DU Q P, CHEN A Z, TIAN J G, et al. Study on method for rapid determination of mercury in traditional Chinese medicines[J]. Chinese Pharmaceutical Affairs, 2010, 24(12): 1175-1177.
[12]	肖情, 陈琳, 李雨晴, 等. 汞离子电化学传感器的研究与应用[J]. 化学传感器,2019,38(2):41−50. [XIAO Q, CHEN L, LI Y Q, et al. Research and application of electrochemical sensor for mercury ion[J]. Chemical Sensors,2019,38(2):41−50. XIAO Q, CHEN L, LI Y Q, et al. Research and application of electrochemical sensor for mercury ion[J]. Chemical Sensors, 2019, 38(2): 41-50.
[13]	柳心梅, 田巍, 冯春梅, 等. 重金属镉特异性抗体的制备及icELISA检测方法的建立[J]. 现代食品科技,2020,36(8):325−332. [LIU X H, TIAN W, FENG C M, et al. Preparation of specific antibody to cadmium and establishment of icELISA detection method[J]. Modern Food Science & Technology,2020,36(8):325−332. LIU X H, TIAN W, FENG C M, et al. Preparation of specific antibody to cadmium and establishment of icELISA detection method[J]. Modern Food Science & Technology, 2020, 36(8): 325-332.
[14]	管怡晗, 黎广进, 刘盛华, 等. 汞离子比色型荧光探针的合成与性质[J]. 环境化学,2021,40(8):2544−2550. [GUAN Y H, LI G J, LIU C H, et al. Synthesis and properties of colorimetric fluorescent probe for mercury ions[J]. Environmental Chemistry,2021,40(8):2544−2550. GUAN Y H, LI G J, LIU C H, et al. Synthesis and properties of colorimetric fluorescent probe for mercury ions[J]. Environmental Chemistry, 2021, 40(8): 2544-2550.
[15]	ZHANG M, LIU F, KE X, et al. Polyaspartamide fluorescent probe containing rhodamine B and sulfadiazine groups for molecular imaging and diagnosis[J]. Chinese Journal of Organic Chemistry,2020,40(4):938. doi: 10.6023/cjoc201908003
[16]	ZHANG K, ZHANG J M. A fluorescent probe for the detection of Hg²⁺ based on rhodamine derivative and modified CdTe quantum dots[J]. Research on Chemical Intermediates,2020(46):987−997.
[17]	JANSSEN C. Heavy metal extractions from NaCl brines to pseudoprotic ionic liquids[J]. Industrial & Engineering Chemistry Research,2021,60(4):1808−1816.
[18]	PRADEEP K, ASHOK K. Extraction of uranium from aqueous solution of nitric acid and organic solvent using ionic liquid[J]. Asian Journal of Chemistry: An International Quarterly Research Journal of Chemistry,2021,33(1):43−48.
[19]	IMDAD S, DOHARE R K. A critical review on heavy metals removal using ionic liquid membranes from the industrial wastewater[J]. Chemical Engineering and Processing - Process Intensification,2022,173:108812−1-108812-13. doi: 10.1016/j.cep.2022.108812
[20]	HANOOO H D, KOWSARI E, ABDOUSS M, et al. Efficient preparation of acidic ionic liquid-functionalized reduced graphene oxide and its catalytic performance in synthesis of benzimidazole derivatives[J]. Research on Chemical Intermediates,2017,43(3):1751−1766. doi: 10.1007/s11164-016-2727-0
[21]	王璐, 张珍, 洪霞, 等. 混合型功能离子液体用于复杂基质样品中汞离子检测的研究[J]. 云南化工,2018,45(10):91−93. [WANG L, ZHANG Z, HONG X, et al. Study on mercury ion detection in complex matrix samples by mixed functional ionic liquids[J]. Yunnan Chemical Technology,2018,45(10):91−93. doi: 10.3969/j.issn.1004-275X.2018.10.035 WANG L, ZHANG Z, HONG X, et al. Study on mercury ion detection in complex matrix samples by mixed functional ionic liquids[J]. Yunnan Chemical Technology, 2018, 45(10): 91-93. doi: 10.3969/j.issn.1004-275X.2018.10.035
[22]	CHEN D D, MAO H L, HONG Y N. Hexaphenyl-1, 3-butadiene derivative: A novel ''turn-on'' rapid fluorescent probe for intraoperative pathological diagnosis of hepatocellular carcinoma[J]. Materials Chemistry Frontiers,2021,4(9):2716−2722.
[23]	YANG Y, LI R, ZHANG S, et al. A fluorescent nanoprobe based on cell-penetrating peptides and quantum dots for ratiometric monitoring of pH fluctuation in lysosomes[J]. Talanta,2021,227:122208. doi: 10.1016/j.talanta.2021.122208
[24]	张嘉莹, 赵丹, 曹晨阳, 等. 泡腾辅助-悬浮固化-分散液液微萃取结合液相色谱法测定食醋中多效唑和异丙甲草胺农药残留[J]. 分析试验室,2018,37(11):1282−1284. [ZHANG J Y, ZHAO D, CAO C Y, et al. Effervescent assisted solidification of floating organic droplet dispersion liquid-liquid microextraction combined with HPLC for the detection of pesticides in vinegar[J]. Chinese Journal of Analysis Laboratory,2018,37(11):1282−1284. ZHANG J Y, ZHAO D, CAO C Y, et al. Effervescent assisted solidification of floating organic droplet dispersion liquid-liquid microextraction combined with HPLC for the detection of pesticides in vinegar[J]. Chinese Journal of Analysis Laboratory, 2018, 37(11): 1282-1284.
[25]	张桂芳. 砷斑法对饲料砷中毒含量的测定[J]. 现代畜牧科技,2014(5):227−227. [ZHANG G F. Determination of arsenic poisoning in feed by arsenic spot method[J]. Technical Advisor for Animal Husbandry,2014(5):227−227. ZHANG G F. Determination of arsenic poisoning in feed by arsenic spot method[J]. Technical Advisor for Animal Husbandry, 2014(5): 227-227.
[26]	刘慧, 孙秀兰. 微波消解-ICP-MS法测定红薯中18种元素[J]. 粮食与饲料工业,2021(1):67−71. [LIU H, SUN X L. Determination of 18 elements in sweet potato by microwave digestion ICP MS[J]. Cereal & Feed Industry,2021(1):67−71. LIU H, SUN X L. Determination of 18 elements in sweet potato by microwave digestion ICP MS[J]. Cereal & Feed Industry, 2021(1): 67-71.

Supplements (0)

Cited By

Cited by

Periodical cited type(2)

1.	朱秀娟，孙娜，陈文东，聂龙英，王林林. 核桃花护色保脆技术研究. 宁夏师范学院学报. 2022(07): 52-58 .
2.	王纪辉，曾亚军，侯娜，耿阳阳，胡伯凯，刘亚娜，张时馨，杨光. 曲面响应法优化核桃雄花多酚提取工艺及其组成与抗氧化活性分析. 南方农业学报. 2022(10): 2997-3008 .