Analysis of Chemical Constituents in Percolate the Extract of <i>Cyclocarya paliurus</i> Tender Leaves by UHPLC-Q-TOF-MS/MS

Boji MA; Yan XIAO; Zude CHEN; Rengeng SHU; Bingtao LI; Li JIANG; Guoliang XU; Qiyun ZHANG

doi:10.13386/j.issn1002-0306.2022070294

Volume 44 Issue 13

Jun. 2023

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Science and Technology of Food Industry > 2023 > 44(13): 281-291. > DOI: 10.13386/j.issn1002-0306.2022070294

MA Boji, XIAO Yan, CHEN Zude, et al. Analysis of Chemical Constituents in Percolate the Extract of Cyclocarya paliurus Tender Leaves by UHPLC-Q-TOF-MS/MS[J]. Science and Technology of Food Industry, 2023, 44(13): 281−291. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022070294.

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Analysis of Chemical Constituents in Percolate the Extract of Cyclocarya paliurus Tender Leaves by UHPLC-Q-TOF-MS/MS

Boji MA^1,,
Yan XIAO¹,
Zude CHEN¹,
Rengeng SHU²,
Bingtao LI^{1, 3},
Li JIANG^{1, 3, 4},
Guoliang XU^{1, 3, 4, ,},
Qiyun ZHANG^{1, 3, ,}

1.
Research Center for Differentiation and Development of Traditional Chinese Medicine (TCM) Basic Theory, Jiangxi University of Chinese Medicine, Nanchang 330004, China
2.
School of Pharmacy, Jiangxi University of Chinese Medicine, Nanchang 330004, China
3.
Jiangxi Provincial Key Laboratory of TCM Etiopathogenisis, Nanchang 330004, China
4.
Key Laboratory of Pharmacology of Traditional Chinese Medicine in Jiangxi, Nanchang 330004, China

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Received Date: July 31, 2022
Available Online: May 06, 2023

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Abstract

Abstract

To analyze the chemical components of the tender leaves of Cyclocarya paliurus, a rapid qualitative analysis of the chemical components of the percolation extracts of the tender leaves of Cyclocarya paliurus was carried out by ultrahigh-performance liquid chromatography coupled with quadrupole-time-of-flight tandem mass spectrometry (UHPLC-Q-TOF-MS/MS). Mass spectrum data were collected in the positive and negative ion modes, and MassHunter qualitative analysis software was used, according to the retention time and mass spectrum information of each compound, combined with existing literature and mass spectrum databases, the compounds were analyzed for the fragmentation rule, 94 compounds were identified from Cyclocarya paliurus, including 29 flavonoids, 16 triterpenes, 25 organic acids, 24 other compounds. The analysis results showed that the percolation extract of Cyclocarya paliurus contained flavonoids, organic acids, triterpenoids, coumarins, amino acids, and other compounds, and malic acid, hesperidin, rosin, 6,7-dihydroxycoumarin, 6-methylcoumarin, 5,7-dimethoxycoumarin are found in Cyclocarya paliurus for the first time. This study can lay a foundation for further clarifying the material basis of Cyclocarya paliurus. It provides a theoretical basis for the development of relevant products of Cyclocarya paliurus.

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