QIN Yu, HUA Zong, ZHANG Min, et al. Optimization of Decolorization and Deproteinization of Balanophora japonica Makino Polysaccharide by Response Surface Methodology[J]. Science and Technology of Food Industry, 2023, 44(10): 177−184. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022070206.
Citation: QIN Yu, HUA Zong, ZHANG Min, et al. Optimization of Decolorization and Deproteinization of Balanophora japonica Makino Polysaccharide by Response Surface Methodology[J]. Science and Technology of Food Industry, 2023, 44(10): 177−184. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022070206.

Optimization of Decolorization and Deproteinization of Balanophora japonica Makino Polysaccharide by Response Surface Methodology

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  • Received Date: July 18, 2022
  • Available Online: March 12, 2023
  • To study the decolorization and deproteinization conditions of Balanophora japonica polysaccharide by hydrogen peroxide and Sevag method, and to optimize the process. Based on single factor experiments, the response surface methodology was used to design a three factor and three level experiments to optimize the decolorization conditions of Balanophora japonica polysaccharides using the decolorization rate of polysaccharides as an index. Using protein removal rate and polysaccharide retention rate as indicators, a response surface experiment was designed at the factor level of Sevag reagent ratio (chloroform:n-butanol), sample solution: Sevag reagent and oscillation time to obtain the optimal process conditions. Results showed that, under the condition of pH8, the optimal decolorization process was as follows: The dosage of hydrogen peroxide was 20%, the decolorization time was 46 min, and the decolorization temperature was 60 ℃. Under this condition, the decolorization rate of polysaccharides was 84.21%. The optimal deproteinization conditions were chloroform:n-butanol 5:1, sample solution:Sevag reagent 4:1, and shock time 15 min, under this condition, the polysaccharide retention rate was 81.03%, and the protein removal rate was 40.44%. The decolorization and deproteinization of Balanophora japonica polysaccharide by hydrogen peroxide and Sevag method was stable, feasible and simple, and would be suitable for decolorization and deproteinization of Balanophora japonica polysaccharide.
  • [1]
    陶汝俊, 徐湘婷. 蛇菰属植物化学成分和药理活性研究进展[J]. 中国民族民间医药,2017,26(7):73−78. [TAO R J, XU X T. Research progress on chemical constituents and pharmacological activities of zizania SPP[J]. Chinese Ethnic Folk Medicine,2017,26(7):73−78.
    [2]
    思茅地区民族传统医药研究所. 拉祜族常见药[M]. 昆明: 云南民族出版社, 1986.

    Simao Institute of Traditional Ethnic Medicine. Chinese Herbal medicine of lahu nationality [M]. Kunming: Yunnan Nationalities Publishing House, 1986.
    [3]
    庄远杯, 林大都, 李云霞, 等. 红冬蛇菰的研究概况[J]. 中国民族民间医药,2017,26(8):60−62. [ZHUANG Y B, LIN D D, LI Y X, et al. Research status of wild mushroom[J]. Chinese Folk Medicine,2017,26(8):60−62.
    [4]
    Editorial Board of China Herbal, State Administration of Traditional Chinese Medicine, China. China Herbal[M]. Shanghai: Shanghai Scientific and Technical Publishers, 1999.
    [5]
    LIN T L, SHU C C, CHEN Y M, et al. Like cures like: Pharmacological activity of anti-inflammatory lipopolysaccharides from gut microbiome[J]. Frontiers in Pharmacology,2020,11:554. doi: 10.3389/fphar.2020.00554
    [6]
    彭梅, 张振东, 杨娟. 14种多糖对小鼠S_(180)肉瘤抑制活性筛选[J]. 山地农业生物学报,2011,30(1):56−59. [PENG M, ZHANG Z D, YANG J. Screening of 14 polysaccharides against S_(180) sarcoma in mice[J]. Journal of Mountain Agricultural Biology,2011,30(1):56−59. doi: 10.3969/j.issn.1008-0457.2011.01.012
    [7]
    WANG X, HUO X Z, LIU Z, et al. Investigations on the anti-aging activity of polysaccharides from Chinese yam and their regulation on klotho gene expression in mice[J]. Journal of Molecular Structure,2020,1208:127895. doi: 10.1016/j.molstruc.2020.127895
    [8]
    LI Q Q, HU J L, NIE Q X, et al. Hypoglycemic mechanism of polysaccharide from Cyclocarya paliurus leaves in type 2 diabetic rats by gut microbiota and host metabolism alteration[J]. Science China Life Sciences,2020,64:117−132.
    [9]
    杨庆伟, 王芃, 全迎萍. 灰树花子实体多糖硫酸酯化及抗凝血活性研究[J]. 北京联合大学学报,2022,36(2):52−56. [YANG Q W, WANG P, QUAN Y P. Study on sulfation and anticoagulant activity of polysaccharides from fruiting body of grifola frondosa[J]. Journal of Beijing union university,2022,36(2):52−56.
    [10]
    陶遵威, 郑夺, 邸明磊, 等. 植物多糖的研究进展[C]//中药及天然产物与健康滨海论坛论文集, 2010: 148-152.

    TAO Z W, ZHENG D, DI M L, et al. Research progress of plant polysaccharides [C]// Journal of Traditional Chinese Medicine, Natural Products and Healthy Coastal Forum, 2010: 148-152.
    [11]
    石雪萍. 植物多糖研究进展[J]. 粮食与油脂,2005,18(8):8. [SHI X P. Research progress of the plant polysaccharides[J]. Grain and Oils,2005,18(8):8. doi: 10.3969/j.issn.1008-9578.2005.08.002
    [12]
    李倩倩, 陈贵元. 刺梨果多糖提取过程中脱蛋白和脱色方法研究[J]. 安徽农业科学,2022,50(11):162−166, 183. [LI Q Q, CHEN G Y. Study on the methods of deproteinization and decolorization of polysaccharide from Rosa roxerus[J]. Journal of Anhui Agricultural Sciences,2022,50(11):162−166, 183.
    [13]
    李洁, 陈琳, 刘海棠, 等. 银条菜粗多糖脱蛋白的方法研究[J]. 天津科技大学学报,2022,37(1):18−22, 27. [LI J, CHEN L, LIU H T, et al. Study on the method of deproteinization of crude polysaccharide from Agaricus japonicus[J]. Journal of Tianjin University of Science and Technology,2022,37(1):18−22, 27.
    [14]
    郭慧静, 张伟达, 陈国刚. 蒲公英多糖脱色脱蛋白方法及其降血糖活性研究[J]. 食品研究与开发,2020,41(3):24−28. [GUO H J, ZHANG W D, CHEN G G. Study on decolorization and deproteinization of dandelion polysaccharide and its hypoglycemic activity[J]. Food Research and Development,2020,41(3):24−28.
    [15]
    何美佳, 刘晓, 唐翠翠, 等. 多糖脱蛋白方法的研究进展[J]. 中国海洋药物,2019,38(3):82−86. [HE M J, LIU X, TANG C C. Research progress of polysaccharide deproteinization methods[J]. China Marine Medicine,2019,38(3):82−86.
    [16]
    付学鹏, 杨晓杰. 植物多糖脱色技术的研究[J]. 食品研究与开发,2007,28(11):166−169. [FU X P, YANG X J. Study on decolorization technology of plant polysaccharides[J]. Food Research and Development,2007,28(11):166−169. doi: 10.3969/j.issn.1005-6521.2007.11.051
    [17]
    车向前, 常明泉. 中药植物色素脱色工艺的应用[J]. 实用药物与临床,2017,20(8):979−982. [CHE X Q, CHANG M Q. Application of plant pigment decolorization technology for traditional Chinese medicine[J]. Practical Drugs and Clinic,2017,20(8):979−982.
    [18]
    孙莹, 纪跃芝, 马爱民, 等. 水提-醇沉法提取大黄多糖工艺优化研究[J]. 中国实用医药,2010,5(18):6−8. [SUN Y, JI Y Z, MA A M, et al. Optimization of rhubarb polysaccharide extraction by water extraction and alcohol precipitation[J]. China Practical Medicine,2010,5(18):6−8.
    [19]
    赵艳, 王白娟, 杨青松等. 红雪茶多糖过氧化氢脱色工艺优化[J]. 南方农业学报,2016,47(5):710−714. [ZHAO Y, WANG B J, YANG Q S, et al. Optimization of hydrogen peroxide decolorization technology of red snow tea polysaccharide[J]. Journal of Southern Agriculture,2016,47(5):710−714. doi: 10.3969/j:issn.2095-1191.2016.05.710
    [20]
    杨勤, 谷文超, 周浓, 等. 苯酚-硫酸法与蒽酮-硫酸法测定地参多糖的比较研究[J]. 食品科技,2020,45(1):343−350. [YANG Q, GU W C, ZHOU N, et al. Comparative study of phenol-sulfuric acid method and anthrone-sulfuric acid method for the determination of polysaccharides from radix ginseng[J]. Food Science and Technology,2020,45(1):343−350.
    [21]
    谢心文, 门磊, 孙怡, 等. 蒽酮-硫酸法测定复方木鸡颗粒中粗多糖[J]. 中成药,2019,41(7):1685−1687. [XIE X W, MEN L, SUN Y, et al. Determination of crude polysaccharide in compound muji granules by anthrone-sulfuric acid method[J]. Chinese Patent Medicine,2019,41(7):1685−1687. doi: 10.3969/j.issn.1001-1528.2019.07.039
    [22]
    杨静, 白冰, 王宁, 张改红. 考马斯亮蓝法对烟草薄片涂布液中蛋白质含量的测定[J]. 湖北农业科学,2017,56(5):946−947,950. [YANG J, BAI B, WANG N, ZHANG G H. Determination of protein content in tobacco coating liquid by coomassie brilliant blue method[J]. Hubei Agricultural Sciences,2017,56(5):946−947,950.
    [23]
    汪荣斌, 秦亚东, 周娟娟. 青钱柳叶多糖双氧水脱色工艺研究[J]. 辽宁中医药大学学报,2016,18(9):60−62. [WANG R B, QIN Y D, ZHOU J J. Study on decolorization technology of polysaccharides from leaves of Cyrrhizoma chinensis[J]. Journal of Liaoning University of Traditional Chinese Medicine,2016,18(9):60−62.
    [24]
    刘冲英, 蒲定涛, 任佳妮, 等. 陕产地黄多糖的纯化及抗氧化活性研究[J]. 化学与生物工程,2020,37(10):15−21. [LIU C Y, PU D T, REN J N, et al. Purification and antioxidant activity of yellow polysaccharide from Shaanxi[J]. Chemical and Biological Engineering,2020,37(10):15−21. doi: 10.3969/j.issn.1672-5425.2020.10.004
    [25]
    经嘉, 廖启元. 猫爪草多糖双氧水脱色工艺研究[J]. 济宁医学院学报,2018,41(6):384−387. [JING J, LIAO Q Y. Study on the decolorization process of polysaccharide from catenopus rubra with hydrogen peroxide[J]. Journal of Jining Medical College,2018,41(6):384−387. doi: 10.3969/j.issn.1000-9760.2018.06.002
    [26]
    张萍, 贺茂萍, 殷力, 葛秋萍. 石榴皮多糖的Sevage法除蛋白工艺研究[J]. 食品科技,2013,38(12):219−222, 231. [ZHANG P, HE M P, YIN L, GE Q P. Study on removal of polysaccharides from pomegranate peel by Sevage method[J]. Food Science and Technology,2013,38(12):219−222, 231. doi: 10.13684/j.cnki.spkj.2013.12.049
    [27]
    曾杰, 房海灵, 梁呈元, 等. 无花果粗多糖脱色工艺优化及其吸附性能研究[J]. 中成药,2022,44(2):537−542. [ZENG J, FANG H L, LIANG C Y, et al. Study on decolorization process optimization and adsorption performance of crude polysaccharide from FIG[J]. Chinese Patent Medicine,2022,44(2):537−542.
    [28]
    亓希武, 房海灵, 陈泽群, 梁呈元, 吕寒. 桃胶多糖脱蛋白工艺研究[J]. 现代食品,2021(8):104−107. [QI X W, FANG H J, CHENG Z Q, et al. Study on deproteinization technology of peach gum polysaccharide[J]. Modern Food,2021(8):104−107.
    [29]
    王乐, 靳学远, 霍智文. 桑枝多糖酶法脱蛋白工艺优化及理化特性研究[J]. 中国食品添加剂,2022,33(7):136−143. [WANG L, JIN X Y, HUO Z W. Study on optimization of enzymatic deproteinization process and physicochemical properties of polysaccharides from mulberry branch[J]. China Food Additives,2022,33(7):136−143.
    [30]
    王莹, 李锋涛, 黄美子, 等. 黄精多糖脱蛋白工艺及其抗氧化活性[J]. 食品工业,2021,42(12):57−62. [WANG Y, LI F T, HHUANG M Z, et al. Deproteinization process and antioxidant activity of polysaccharides from Rhizoma polygonatum[J]. Food Industry,2021,42(12):57−62.
    [31]
    刘冲英, 陈子龙, 王睿, 等. 地黄多糖脱蛋白工艺研究[J]. 化学与生物工程,2020,37(11):46−50. [LIU C Y, CHEN Z L, WANG R, et al. Study on deproteinization of polysaccharides from rehmannia glutinosa[J]. Chemical and Biological Engineering,2020,37(11):46−50.
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