Microbial Diversity Analysis of Sufu from Different Origins Based on High-throughput Sequencing

FU Ruimin; LIU Chunlei; XU Liang; ZHANG Hong; XIA Tieqi; CHEN Wuling

doi:10.13386/j.issn1002-0306.2022030065

Volume 44 Issue 2

Jan. 2023

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Science and Technology of Food Industry > 2023 > 44(2): 134-142. > DOI: 10.13386/j.issn1002-0306.2022030065

FU Ruimin, LIU Chunlei, XU Liang, et al. Microbial Diversity Analysis of Sufu from Different Origins Based on High-throughput Sequencing[J]. Science and Technology of Food Industry, 2023, 44(2): 134−142. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022030065.

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Microbial Diversity Analysis of Sufu from Different Origins Based on High-throughput Sequencing

1.
College of Sport and Health Management, Henan Finance University, Zhengzhou 450046, China
2.
College of Food Science, Shaanxi Normal University, Xi'an 710069, China

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Received Date: March 06, 2022
Available Online: November 21, 2022

Graphical Abstract

Abstract

Abstract

To learn about the diversity of microorganisms in different flavor sufu from different origins in China. Based on high throughput sequencing (HTS), the microbial diversity differences and chemical properties of 16 sufu samples from different origins in China were analyzed in this study. The results of bacterial diversity showed that the most abundant bacteria in sufu were Lactobacillus sp and Bacillus sp, which also represented the dominant bacterial communities in different stages of sufu fermentation. The results of fungal diversity showed that sufu contained a large number of Aspergillus, Actinmucor sp. and other hydrolase producing bacteria. The results of chemical composition determination showed that different origins had certain influence on the chemical composition of sufu. The soluble protein content and amino nitrogen content in curd showed positive correlation with the distribution of Bacillus in the samples. The results of principal coordinate analysis showed that the fungal consortium structure in sufu samples from 16 origins had certain clustering relationship with regions, specifically the fungi from eastern China (ZJ2, ZJ4, ZJ5, ZJ6, SH9, AH11 and JX12) could be grouped together, which stated that the fungal consortium of sufu may be influenced by geographical factors. This study deepened the understanding of microbial community diversity in sufu and would provide theoretical support for the quality improvement of traditional fermented condiments.
- high-throughput sequencing,
- sufu,
- principal coordinate analysis (PcoA),
- microbial diversity,
- different producing areas,
- bacteria,
- fungus

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References (29)

References

[1]	张鹏飞, 乌日娜, 武俊瑞. 传统发酵大豆制品挥发性成分和微生物多样性的研究进展[J]. 中国酿造,2018,37(12):1−6. [ZHANG Pengfei, WU Rina, WU Junrui. Research advance on volatile components and microbial diversity of traditional fermented soybean products[J]. China Brewing,2018,37(12):1−6. doi: 10.11882/j.issn.0254-5071.2018.12.001
[2]	孙娜, 张雅婷, 于寒松, 等. 发酵型青腐乳菌群结构与风味物质及其相关性分析[J]. 食品科学,2020,41(22):177−183. [SUN Na, ZHANG Yating, YU Hansong, et al. Microflora structure and flavor components and correlation between them in fermented stinky tofu[J]. Food Science,2020,41(22):177−183. doi: 10.7506/spkx1002-6630-20190929-360
[3]	TAN G, HU M, LI X, et al. High-throughput sequencing and metabolomics reveal differences in bacterial diversity and metabolites between red and white sufu[J]. Frontiers in Microbiology,2020,11:758−760. doi: 10.3389/fmicb.2020.00758
[4]	LIU S, QIAO J. Bacterial diversity of Anshun sufu, a traditional fermented tofu in Guizhou province of China[J]. Transactions of Tianjin University,2019,25(5):497−503. doi: 10.1007/s12209-019-00198-8
[5]	YAO D, XU L, WU M, et al. Effects of microbial community succession on flavor compounds and physicochemical properties during CS sufu fermentation[J]. LWT,2021,152:112313. doi: 10.1016/j.lwt.2021.112313
[6]	TAN G, HU M, LI X, et al. Metagenomics reveals the diversity and taxonomy of antibiotic resistance genes in sufu bacterial communities[J]. Food Control,2021,121:107641. doi: 10.1016/j.foodcont.2020.107641
[7]	LAW K K L, MAKINO S, MO R, et al. Antagonistic and cooperative actions of Kif7 and sufu define graded intracellular Gli activities in Hedgehog signaling[J]. PloS One,2012,7(11):e50193. doi: 10.1371/journal.pone.0050193
[8]	HE W, CHUNG H Y. Exploring core functional microbiota related with flavor compounds involved in the fermentation of a natural fermented plain sufu (Chinese fermented soybean curd)[J]. Food Microbiology,2020,90:103408. doi: 10.1016/j.fm.2019.103408
[9]	徐琼, 刘洋, 曲勤凤, 等. 高通量测序分析不同地区红腐乳细菌多样性[J]. 食品科学,2020,41(10):110−116. [XU Qiong, LIU Yang, QU Qinfeng, et al. High-throughput sequencing analysis of bacterial diversity in red sufu from different regions[J]. Food Science,2020,41(10):110−116. doi: 10.7506/spkx1002-6630-20190408-087
[10]	余巨全, 巩建华, 柯尊伟. 对中国黄牛毛囊DNA六种提取方法的研究[J]. 基因组学与应用生物学,2020,39(4):1540−1548. [YU Juquan, GONG Jianhua, KE Zunwei. Study on six methods of extracting DNA from hair follicle of Bos taurina[J]. Genetics and Applied Biology,2020,39(4):1540−1548.
[11]	WAN Y, WANG Y, SHI Z, et al. Wheat amino acid transporters highly expressed in grain cells regulate amino acid accumulation in grain[J]. PloS One,2021,16(2):e0246763. doi: 10.1371/journal.pone.0246763
[12]	蒋丽婷, 李理. 白腐乳质构与其成分相关性研究[J]. 现代食品科技,2010,26(8):797−800. [JIANG Liting, LI Li. The relation of chemical component to texture of white sufu[J]. Modern Food Science and Technology,2010,26(8):797−800.
[13]	代来鑫, 卢红梅, 张丽平, 等. 影响腐乳结晶物产生的因素[J]. 食品与发酵工业,2013,39(7):114−119. [DAI Laixin, LU Hongmei, ZHANG Liping, et al. Effectors that influence the generation of crystal in sufu[J]. Food and Fermentation Industries,2013,39(7):114−119. doi: 10.13995/j.cnki.11-1802/ts.2013.07.042
[14]	陶康, 吴凌伟, 金晓芳, 等. 基于高通量基因测序分析腐乳微生物多样性[J]. 食品科学,2021,42(8):143−149. [TAO Kang, WU Lingwei, JIN Xiaofang, et al. Analysis of microbial diversity in sufu using high-throughput sequencing[J]. Food Science,2021,42(8):143−149. doi: 10.7506/spkx1002-6630-20191230-362
[15]	LIANG J, LI D, SHI R, et al. Effects of microbial community succession on volatile profiles and biogenic amine during sufu fermentation[J]. LWT,2019,114:108379. doi: 10.1016/j.lwt.2019.108379
[16]	XIE C, ZENG H, WANG C, et al. Volatile flavour components, microbiota and their correlations in different sufu, a Chinese fermented soybean food[J]. Journal of Applied Microbiology,2018,125(6):1761−1773. doi: 10.1111/jam.14078
[17]	FENG Z, GAO W, REN D, et al. Evaluation of bacterial flora during the ripening of Kedong sufu, a typical Chinese traditional bacteria-fermented soybean product[J]. Journal of the Science of Food and Agriculture,2013,93(6):1471−1478. doi: 10.1002/jsfa.5918
[18]	张雅婷, 孙娜, 于寒松, 等. 红腐乳发酵过程中菌群结构与风味相关性研究进展[J]. 食品与发酵工业,2020,46(1):287−294. [ZHANG Yating, SUN Na, YU Hansong, et al. Advances in research on correlation between flora structure and product flavor during red sufu fermentation[J]. Food and Fermentation Industries,2020,46(1):287−294.
[19]	YAO D, XU L, WU M, et al. Microbial community succession and metabolite changes during fermentation of BS sufu, the fermented black soybean curd by Rhizopus microsporus, Rhizopus oryzae, and Actinomucor elegans[J]. Frontiers in Microbiology,2021,12:1505−1506.
[20]	周小虎, 李理. 白腐乳发酵过程中细菌群落演替规律研究[J]. 中国酿造,2020,39(5):168−172. [ZHOU Xiaohu, LI li. Succession regulations of bacterial community in the fermentation process of white sufu[J]. China Brewing,2020,39(5):168−172. doi: 10.11882/j.issn.0254-5071.2020.05.032
[21]	ZHEN D Z, Y R W, FAN S X, et al. Distinct bacterial community of a solid-state fermented Chinese traditional food huase sufu revealed by high-throughput sequencing[J]. Food Science and Biotechnology,2021,30(9):1233−1241. doi: 10.1007/s10068-021-00963-3
[22]	SHI C, LIU M, ZHAO H, et al. Formation and control of biogenic amines in sufu-a traditional Chinese fermented soybean product: A critical review[J]. Food Reviews International,2021:1−22.
[23]	XU D, WANG P, ZHANG X, et al. High-throughput sequencing approach to characterize dynamic changes of the fungal and bacterial communities during the production of sufu, a traditional Chinese fermented soybean food[J]. Food Microbiology,2020,86:103340. doi: 10.1016/j.fm.2019.103340
[24]	陈卓, 吴学凤, 穆冬冬, 等. 红腐乳后酵期风味物质与细菌菌群分析[J]. 食品科学,2021,42(6):118−125. [CHEN Zhuo, WU Xuefeng, MU Dongdong, et al. Determination and correlation analysis of flavor components with bacterial community in post-fermented red sufu[J]. Food Science,2021,42(6):118−125. doi: 10.7506/spkx1002-6630-20191203-043
[25]	LI K, TANG J, ZHANG Z, et al. Correlation between flavor compounds and microorganisms of chaling natural fermented red sufu[J]. LWT,2022,154:112873. doi: 10.1016/j.lwt.2021.112873
[26]	万红芳, 赵勇, 王正全, 等. 生产菌种及环境微生物与腐乳品质关系研究进展[J]. 食品与发酵工业,2019,45(6):255−261. [WAN Hongfang, ZHAO Yong, WANG Zhengquan, et al. Research progress on the relationship between sufu quality and microorganisms from starters and environment[J]. Food and Fermentation Industries,2019,45(6):255−261.
[27]	李娜, 崔梦君, 马佳佳, 等. 自然发酵腐乳中细菌多样性评价[J]. 食品研究与开发,2019,40(16):165−171. [LI Na, CUI Mengjun, MA Jiajia, et al. Evaluation of bacterial diversity in natural fermented sufu[J]. Food Research and Development,2019,40(16):165−171. doi: 10.12161/j.issn.1005-6521.2019.16.029
[28]	GU J S, LIU T J, HOU J, et al. Analysis of bacterial diversity and biogenic amines content during the fermentation processing of stinky tofu[J]. Food Research International,2018,111:689−698. doi: 10.1016/j.foodres.2018.05.065
[29]	LI X, HE Y, YANG W, et al. Comparative analysis of the microbial community and nutritional quality of sufu[J]. Food Science & Nutrition,2021,9(8):4117−4126.

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