WANG Jialuan, ZHAO Fengyi, WU Wenlong, et al. Correlation Analysis of Ellagic Acid with Antioxidant and Carbohydrate Substances in Different Development Stages of Blackberry and Raspberry Leaves[J]. Science and Technology of Food Industry, 2023, 44(2): 75−82. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022030049.
Citation: WANG Jialuan, ZHAO Fengyi, WU Wenlong, et al. Correlation Analysis of Ellagic Acid with Antioxidant and Carbohydrate Substances in Different Development Stages of Blackberry and Raspberry Leaves[J]. Science and Technology of Food Industry, 2023, 44(2): 75−82. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022030049.

Correlation Analysis of Ellagic Acid with Antioxidant and Carbohydrate Substances in Different Development Stages of Blackberry and Raspberry Leaves

More Information
  • Received Date: March 03, 2022
  • Available Online: November 09, 2022
  • The purpose of this study was to preliminarily reveal the changes of ellagic acid in leaves of Rubus berries at different developmental stages. In this study, leaves of 21 varieties of blackberry, raspberry and the hybrids were used to extract the ellagic acid in the leaves by ultrasonic assisted solvent extraction method, and the content of ellagic acid was rapidly detected by UV spectrophotometry. On this basis, five varieties were selected to analyze and evaluate the relationship between antioxidant capacity, antioxidant content, carbohydrate content and ellagic acid content. The results showed that the content of ellagic acid in the tender leaves of blackberry and raspberry was higher, and it showed a downward trend with the development and aging of the leaves. The content of ellagic acid in the tender leaves of blackberry cv. 'Baosen' was the highest up to 39.622 mg/g, and the content of ellagic acid in the aging leaves of raspberry 'Haritez' was the lowest, which was 7.649 mg/g. Furthermore, the antioxidant capacity and antioxidant content of the leaves of the selected five varieties were analyzed. It was found that the change trend was similar to that of ellagic acid, while the change of carbohydrate content in the leaves was negatively correlated with that of ellagic acid. The results would provide some theoretical basis for the functional research and further development and utilization of ellagic acid in blackberry and raspberry leaves.
  • [1]
    吴文龙, 顾姻. 新经济植物黑莓的引种[J]. 植物资源与环境,1994(3):45−48. [WU W L, GU Y. Blackberry introduction and cultivation[J]. Journal of Plant Resources and Environment,1994(3):45−48.
    [2]
    孙醉君, 顾姻, 蔡剑华. 黑莓引种十年的回顾与展望[J]. 江苏林业科技,1998(3):48−50, 56. [SUN Z J, GU Y, CAI J H. Review and prospect of ten years introduction of blackberry[J]. Journal of Jiangsu Forestry Science and Technology,1998(3):48−50, 56.
    [3]
    TAMAR T, MERAB J, LEVAN G, et al. Effect of ascorbic acid treatment on some quality parameters of frozen strawberry and raspberry fruits[J]. Annals of Agrarian Science,2017,15(3):370−374. doi: 10.1016/j.aasci.2017.02.017
    [4]
    ROMMEL A, WROLSTAD R. Ellagic acid content of red raspberry juice as influenced by cultivar, processing, and environmental factors[J]. Journal of Agricultural and Food Chemistry,1993,41(11):1951−1960. doi: 10.1021/jf00035a026
    [5]
    张立华, 郝兆祥, 董业成. 石榴的功能成分及开发利用[J]. 山东农业科学,2015,47(10):133−138. [ZHANG L H, HAO Y X, DONG Y C. Functional components and development and utilization of pomegranate[J]. Shandong Agricultural Sciences,2015,47(10):133−138.
    [6]
    USTA, OZDEMIR, SCHIARITI, et al. The pharmacological use of ellagic acid-rich pomegranate fruit[J]. International Journal of Food Sciences and Nutrition,2013,64(7):907−913. doi: 10.3109/09637486.2013.798268
    [7]
    SAYEDALIREZA M, SAYEDMOJTABA M. Benefits of ellagic acid from grapes and pomegranates against colorectal ancer[J]. Caspian Journal of Internal Medicine,2017,8(3):226−227.
    [8]
    XU Y, CHARLES M T, LUO Z, et al. Preharvest ultraviolet C irradiation increased the level of polyphenol accumulation and flavonoid pathway gene expression in strawberry fruit[J]. Journal of Agricultural and Food Chemistry,2017,65(46):9970−9979. doi: 10.1021/acs.jafc.7b04252
    [9]
    DANH V C, PHUC V H, MARK C V, et al. Identification and characterization of phenolic compounds in black walnut kernels[J]. Journal of Agricultural and Food Chemistry,2018,66(17):4503−4511. doi: 10.1021/acs.jafc.8b01181
    [10]
    崔珊珊, 毕凯媛, 吴杰, 等. 响应面法优化树莓鞣花酸提取工艺及其体外抗氧化活性[J]. 食品工业科技,2019,40(1):149−155,161. [CUI S S, BI K Y, WU J, et al. Optimization of extraction technology and antioxidative activity of ellagic acid in raspberry by response surface methodology[J]. Science and Technology of Food Industry,2019,40(1):149−155,161.
    [11]
    蒋新龙, 吴小刚, 郭叶莹, 等. 鞣花酸磷脂复合物制备及其对植物油抗氧化作用[J]. 中国粮油学报,2021,36(2):81−88. [JANG X L, WU X G, GUO Y Y, et al. Preparation of ellagic acid phospholipid complex and its antioxidative effect on vegetable oil[J]. Journal of the Chinese Cereals and Oils Association,2021,36(2):81−88.
    [12]
    NIZAR A, QOSAY A, MOHAMMAD A, et al. Ellagic acid: A potent glyoxalase-I inhibitor with unique scaffold[J]. Acta Pharmaceutica,2021,71(1):115−130. doi: 10.2478/acph-2021-0005
    [13]
    MOUAD E, IRINA O, IZUMI O, et al. Ellagic acid induces apoptosis through inhibition of nuclear factor κB in pancreatic cancer cells[J]. World Journal of Gastroenterology,2008(23):3672−3680.
    [14]
    TAN Y H, SHUDO T, YOSHIDA T, et al. Ellagic acid, extracted from sanguisorba officinalis, induces G1 arrest by modulating PTEN activity in B16F10 melanoma cells[J]. Genes to Cells,2019,24(11):688−704. doi: 10.1111/gtc.12719
    [15]
    钟晨. 鞣花酸抑制肝癌的分子机制及其与两种化疗药物协同作用的研究[D]. 哈尔滨: 东北林业大学, 2019

    ZHONG C. The mechanisms of ellagic acid against hepatocellular carcinoma and synergistic effects of its combinations with two chemotherapeutic agents[D]. Harbin: Northeast Forestry University, 2019.
    [16]
    FATIMA N, HAFIZUR R M, HAMEED A, et al. Ellagic acid in emblica officinalis exerts anti-diabetic activity through the action on β-cells of pancreas[J]. European Journal of Nutrition,2017,56(2):591−601. doi: 10.1007/s00394-015-1103-y
    [17]
    JOZAA Z, NORA A, GHEDEIR M, et al. Ellagic acid protects against diabetic nephropathy in rats by regulating the transcription and activity of Nrf2[J]. Journal of Functional Foods,2021,79:1−15.
    [18]
    GONÇALO G, FARIA P T, PAULA P, et al. Bioaccessible raspberry extracts enriched in ellagitannins and ellagic acid derivatives have anti-neuroinflammatory properties[J]. Antioxidants,2020,9(10):970−970. doi: 10.3390/antiox9100970
    [19]
    吕广. 鞣花酸对脂多糖诱导的炎症反应的抑制作用和机制研究[D]. 长春: 吉林大学, 2016

    LÜ G. Inhibitory effects and mechanisms of ellagic acid on lipopolysaccharide-induced inflammation[D]. Changchun: Jilin University, 2016.
    [20]
    GUPTA A, PANDEY A K. Aceclofenac-induced hepatotoxicity: An ameliorative effect of terminalia bellirica fruit and ellagic acid[J]. World Journal of Hepatology,2020,12(11):949−964. doi: 10.4254/wjh.v12.i11.949
    [21]
    MAR L, MARÍA T, JUAN C. Ellagitannins, ellagic acid and vascular health[J]. Molecular Aspects of Medicine,2010,31(6):513−539. doi: 10.1016/j.mam.2010.09.005
    [22]
    BIALONSKA D, SASHI G, KEVIN K, et al. The effect of pomegranate (Punica granatum L.) byproducts and ellagitannins on the growth of human gut bacteria[J]. Journal of Agricultural and Food Chemistry,2009,57(18):8344−8349. doi: 10.1021/jf901931b
    [23]
    焦士蓉, 唐子尧, 孙博瑞, 等. 石榴皮提取物和鞣花酸对人脂肪干细胞增殖和分化的影响[J]. 西华大学学报(自然科学版),2019,38(2):48−51. [JIAO S R, TANG Z X, SUN B R, et al. Effect of pomegranate peel extract and ellagic acid on human adipose-derived cells proliferation and differentiation[J]. Journal of Xihua University (Natural Science Edition),2019,38(2):48−51.
    [24]
    骆菊文, 蒋新元, 徐佳敏, 等. 鞣花酸的稳定性及其保护研究[J]. 日用化学工业,2020,50(8):547−552. [LUO J W, JIANG X Y, XU J M, et al. Study on the stability and protection of ellagic acid[J]. China Surfactant Detergent & Cosmetics,2020,50(8):547−552.
    [25]
    GILL N K, RIOS D, OSORIO C E, et al. Anticancer effects of extracts from three different chokeberry species[J]. Nutrition and Cancer,2020,73(7):1168−1174.
    [26]
    MUJAWAR S B, MURIGENDRA B. Evaluation of in vitro antioxidant and anti-inflammatory activities of Aegle marmelos leaf extracts[J]. Asian Journal of Pharmaceutical and Clinical Research,2020:209−213.
    [27]
    汪瑞敏, 李朝辉, 赵丰年, 等. 不同溶剂对诺丽籽提取物中多酚构成谱和抗氧化能力、抑菌活性的影响[J]. 食品科技,2021,46(10):169−176. [WANG R M, LI Z H, ZHAO F N, et al. Effect of different extraction solvents on phenolics profile, antioxidant activity and antibacterial activity of noni (Morinda Citrifolia L.) seed extracts[J]. Food Science and Technology,2021,46(10):169−176.
    [28]
    JEFFREY B H, CHRISTINE A W. Advances in flavonoid research since 1992[J]. Phytochemistry,2000,55(6):481−504. doi: 10.1016/S0031-9422(00)00235-1
    [29]
    杨红澎, 李曦, 徐彤, 等. 石榴果实及果皮抗氧化成分的测定[J]. 中国食物与营养,2021,27(9):39−41. [YANG H P, LI X, XU T, et al. Determination on antioxidant components in fruit and peel from Puhica granatum[J]. Food and Nutrition in China,2021,27(9):39−41.
    [30]
    GREBE M, EISELE H J, WEISSMANN N, et al. Antioxidant vitamin C improves endottelial function in obstructive sleep apnea[J]. American Journal of Respiratory and Critical Care Medicine,2006,173(8):897−901. doi: 10.1164/rccm.200508-1223OC
    [31]
    NETH B J, BAUER B A, BENARROCH E E, et al. The role of oxidative stress in Parkinson's disease[J]. Antioxidants,2020,9:597. doi: 10.3390/antiox9070597
    [32]
    ZHANG Y Z, WANG L, ZHANG J J, et al. Vascular peroxide 1 promotes ox-LDL-induced programmed necrosis in endothelial cells through a mechanism involving β-catenin signaling[J]. Atherosclerosis,2018,274:128−138. doi: 10.1016/j.atherosclerosis.2018.04.031
    [33]
    ZHANG Y Q, ARCHANA U, SATHYASEELAN S D, et al. A new role for oxidative stress in aging: The accelerated aging phenotype in Sod1−/− mice is correlated to increased cellular senescence[J]. Redox Biology,2017,11(C):30−37.
    [34]
    何嘉琪, 刘洪霞, 吴文龙, 等. 阿魏酸和那他霉素对采后黑莓果实细胞壁代谢的影响[J]. 北方园艺,2021(12):94−101. [HE J Q, LIU H X, WU W L, et al. Effects of ferulic acid and natamycin on cell wall metabolism of postharves blackberry fruits[J]. Northern Horticulture,2021(12):94−101.
    [35]
    严志祥, 杨海燕, 樊苏帆, 等. 黑莓果实发育过程中蔗糖磷酸合成酶基因的表达分析[J]. 南京林业大学学报(自然科学版),2022,46(1):179−186. [YAN Z X, YANG H Y, FAN S F, et al. Analysis of the expression of sucrose phosphate synthase genes during the development of blackberry fruit[J]. Journal of Nanjing Forestry University (Natural Sciences Edition),2022,46(1):179−186.
    [36]
    杨光, 周双, 孙兰英, 等. HPLC法测定秋果型树莓不同部位鞣花酸的含量[J]. 黑龙江农业科学,2021(3):86−89. [YANG G, ZHOU S, SUN L Y, et al. Determination of ellagic acid in different part of raspberry by HPLC[J]. Heilongjiang Agricultural Science,2021(3):86−89.
    [37]
    LU J, YUAN Q. A new method for ellagic acid production from pomegranate husk[J]. Journal of Food Process Engineering,2008,31(4):443−454. doi: 10.1111/j.1745-4530.2007.00169.x
    [38]
    邸幼军, 贾光锋. 超声强化提取石榴皮鞣花酸的工艺研究[J]. 现代生物医学进展,2010,10(19):3703−3705. [DI Y J, JIA G F. Study on the ultrasonic extraction process of ellagic acid from pomegranate Husk[J]. Progress in Modern Biomedicine,2010,10(19):3703−3705.
    [39]
    王佳慧, 于欣悦, 廖声玲, 等. 树莓中鞣花酸提取工艺的优化[J]. 中国林副特产,2017(4):14−17. [WANG J H, YU X Y, LIAO S L, et al. Optimization of extraction process of ellagic acid from raspberry[J]. Forest By Production and Speciality in China,2017(4):14−17.
    [40]
    陈笳鸿, 吴冬梅, 汪咏梅, 等. 紫外分光光度法测定鞣花酸含量的研究[J]. 生物质化学工程,2007(3):18−20. [CHEN J H, WU D M, WANG Y M, et al. Study on quantitative determ ination of ellagic acid by UV-spectropho tometry[J]. Biomass Chemical Engineering,2007(3):18−20.
    [41]
    李静, 聂继云, 李海飞, 等. Folin-酚法测定水果及其制品中总多酚含量的条件[J]. 果树学报,2008(1):126−131. [LI J, NIE J Y, LI H F, et al. On determination conditions for total polyphenols in fruits and its derived products by Folin- phenol methods[J]. Journal of Fruit Science,2008(1):126−131.
    [42]
    CHEN Z, JIANG J Y, SHU L Z, et al. Combined transcriptomic and metabolic analyses reveal potential mechanism for fruit development and quality control of Chinese raspberry (Rubus chingii Hu)[J]. Plant Cell Reports,2021,40(10):1−24.
    [43]
    BALA I, BHARDWAJ V, HARIHARAN S, et al. Analytical methods for assay of ellagic acid and its solubility studies[J]. Journal of Pharmaceutical and Biomedical Analysis,2006,40(1):206−210. doi: 10.1016/j.jpba.2005.07.006
    [44]
    冯立娟, 尹燕雷, 苑兆和, 等. 石榴叶片发育期安石榴苷及其合成相关物质含量的变化[J]. 果树学报,2016,33(3):314−323. [FENG L J, YI Y L, YUAN Z H, et al. Studies on the contents changes of punicalagin and its synthetic related substance in pomegranate leaf during development[J]. Journal of Fruit Science,2016,33(3):314−323.
    [45]
    MAAS J L, WANG S Y, GALLETTA G J. Evaluation of strawberry cultivars for ellagic acid content[J]. Hortscience,1991,26(1):66−68. doi: 10.21273/HORTSCI.26.1.66
    [46]
    吴妙鸿, 邱珊莲, 林宝妹, 等. 番石榴4个品种叶和幼果的生物活性和酚类成分研究[J]. 热带亚热带植物学报,2021,29(6):694−702. [WU M H, QIU S L, LIN B M, et al. Biological activities and phenolic constituents in extracts from leaves and young fruits of four guava cultivars[J]. Journal of Tropical and Subtropical Botany,2021,29(6):694−702.
    [47]
    TORUNN E T, LINDA H, SIV K B, et al. Polyphenol-rich juices reduce blood pressure measures in a randomised controlled trial in high normal and hypertensive volunteers[J]. British Journal of Nutrition,2015,114(7):1054−1063. doi: 10.1017/S0007114515000562
    [48]
    JOSEPH S V, EDIRISINGHE I, BURTON B M. Berries: Anti-inflammatory effects in humans[J]. Journal of Agricultural and Food Chemistry,2014,62(18):3886−3903. doi: 10.1021/jf4044056
    [49]
    唐莹, 贾仕杰, 蒋莹, 等. 红树莓叶茶的制备及与红树莓叶片、绿茶品质的比较[J]. 食品科学,2021,42(17):34−42. [TANG Y, JIA S J, JIANG Y, et al. Preparation of red raspberry leaf tea and comparison of its quality with red raspberry leaves and commercial green tea[J]. Food Science and Technology,2021,42(17):34−42.
  • Cited by

    Periodical cited type(6)

    1. 徐于珽,于泓博,王晶,高文越,关云鹏,易思彤,任广辉,白长川,朱英. 基于肠肝轴中医药调节肠道功能防治肝细胞癌作用机制的研究进展. 中国中西医结合消化杂志. 2024(03): 222-225+232 .
    2. 闫晓慧,姜思亮,刘雪晴,梁军,夏永刚. 灵芝多糖的构效关系、提取工艺及药理作用研究进展. 中医药学报. 2024(04): 117-122 .
    3. 李秀清,纪宝玉,何江龙,李盼盼,吴婉阁,陈随清,董诚明,裴莉昕. 基于“习性-生境-部位-药性”的菌类中药相关性分析. 中国实验方剂学杂志. 2024(10): 133-139 .
    4. 罗世博,刘伟,谢星,冯亚萍,彭春彦,张露. 紫色野生樱桃李提取物抗氧化和降血糖活性研究及化学成分鉴定. 食品与发酵工业. 2024(19): 191-200 .
    5. 池妍,王雅芝,王昊,汲晨锋. 基于Citespace可视化分析5种真菌多糖研究进展. 中草药. 2024(23): 8130-8152 .
    6. 周美伶,范春艳,王宏艳. 中药多糖在非酒精性脂肪性肝病中的研究进展. 现代畜牧兽医. 2023(12): 89-93 .

    Other cited types(0)

Catalog

    Article Metrics

    Article views PDF downloads Cited by(6)

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return