Preparation and Antioxidant Activity Analysis of Hydrolyzed Peptides from <i>Eucommia ulmoides</i> Seed Meal

GE Zhenzhen; XU Mingyue; ZHAO Yuxiang; ZHAO Guangyuan; ZONG Wei

doi:10.13386/j.issn1002-0306.2022020050

Volume 43 Issue 21

Oct. 2022

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Science and Technology of Food Industry > 2022 > 43(21): 218-224. > DOI: 10.13386/j.issn1002-0306.2022020050

GE Zhenzhen, XU Mingyue, ZHAO Yuxiang, et al. Preparation and Antioxidant Activity Analysis of Hydrolyzed Peptides from Eucommia ulmoides Seed Meal[J]. Science and Technology of Food Industry, 2022, 43(21): 218−224. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022020050.

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Preparation and Antioxidant Activity Analysis of Hydrolyzed Peptides from Eucommia ulmoides Seed Meal

GE Zhenzhen^{1, 2, 3,},
XU Mingyue¹,
ZHAO Yuxiang¹,
ZHAO Guangyuan^{1, 2, 3},
ZONG Wei^{1, 2, 3, ,}

1.
College of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China
2.
Henan Key Laboratory of Cold Chain Food Quality and Safety Control, Zhengzhou 450001, China
3.
Food Production and Safety Henan Collaborative Innovation Center, Zhengzhou 450001, China

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Received Date: February 10, 2022
Available Online: August 29, 2022

Graphical Abstract

Abstract

Abstract

Eucommia ulmoides seed meal protein prepared by alkali extraction and acid precipitation was utilized as raw material. On the basis of single factor experiment, the total evaluation value of the hydrolysis degree and total antioxidant capacity (T-AOC) as response value was used to optimize the addition of enzyme, hydrolysis time and substrate concentration. And the Box-Behnken method was used for three factors and three levels response surface test design to determine the best enzymatic preparation process of hydrolyzed peptides from Eucommia ulmoides seed meal. The antioxidant activity of the obtained hydrolyzed peptides was determined in vitro. The results showed that neutral protease was the best protease, and the best enzymatic hydrolysis condition were enzyme was 10000 U/g, hydrolysis time 1.50 h and substrate concentration 20 g/L. Under these conditions, the degree of hydrolysis and T-AOC of hydrolyzed peptides were 47.45%±1.50% and 30.62±0.59 μmol/g, separately; the IC₅₀ for DPPH free radical, superoxide anion radical and ABTS free radical scavenging rates were 0.731, 4.258 and 0.407 mg/mL, respectively. The Eucommia ulmoides seed meal peptides prepared under these conditions had great antioxidant capacity and provided a theoretical basis for the high-value utilization of Eucommia ulmoides seed meal and the development of antioxidant peptide functional products.
- Eucommia ulmoides seed meal,
- hydrolyzed peptides,
- preparation technology,
- antioxidant activity

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