CHEN Boyang, ZHAO Lingjie, LUO Yunfeng, et al. Study on the Mechanism of Guizhou Rosa roxburghii Radix on Ulcerative Colitis Based on TLR4/NF-κB Signaling Pathway[J]. Science and Technology of Food Industry, 2022, 43(13): 353−358. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021100024.
Citation: CHEN Boyang, ZHAO Lingjie, LUO Yunfeng, et al. Study on the Mechanism of Guizhou Rosa roxburghii Radix on Ulcerative Colitis Based on TLR4/NF-κB Signaling Pathway[J]. Science and Technology of Food Industry, 2022, 43(13): 353−358. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021100024.

Study on the Mechanism of Guizhou Rosa roxburghii Radix on Ulcerative Colitis Based on TLR4/NF-κB Signaling Pathway

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  • Received Date: October 10, 2021
  • Available Online: May 01, 2022
  • Objective: To explore the mechanism of Guizhou Rosa roxburghii Radix in the treatment of ulcerative colitis in rats based on TLR4/NF-κB signaling pathway. Methods: The ulcerative colitis model was established by gavage with 2,4,6-trinitrobenzenesulfonic acid (TNBS)/ethanol solution, gavage with Rosa roxburghii Radix aqueous decoction high, medium and low dose groups (8, 4 and 2 g/kg) and salazosulfanil group (0.3 g/kg). Observation of the appearance, motor behaviour and blood stool of the rats. Serum and colon of rats were collected, and hematoxylin-eosin (HE) staining was used to observe the pathological changes in the colon of each group of rats. Enzyme-linked immunosorbent assay (ELISA) for the detection of serum interleukin (IL)-1β, IL-6, TNF-α levels in rats. Reverse transcription-polymerase chain reaction (RT-PCR) for the detection of rat colon Myd88, NF-κB p50, NF-κB p65, TLR4 mRNA expression. Protein immunoblotting (Western blot) was performed to detect Myd88, NF-κB p50, NF-κB p65, TLR4 protein expression in rat colon. Results: Rosa roxburghii Radix aqueous decoction significantly improved colonic inflammatory injury in rats with ulcerative colitis, especially in the high dose group of Rosa roxburghii Radix aqueous decoction. Compared with the model group, the Rosa roxburghii Radix aqueous decoction high-dose group showed significant improvement in colon pathological damage. Serum IL-1β, IL-6 and TNF-α levels were significantly decreased in the Rosa roxburghii Radix aqueous decoction high-dose group (P<0.01); colon Myd88, NF-κB p50, TLR4 mRNA expression was significantly decreased (P<0.05); colon Myd88, NF-κB p50, NF-κB p65, TLR4 protein expression was significantly decreased (P<0.01). Conclusion: Rosa roxburghii Radix aqueous decoction can effectively alleviate TNBS-induced ulcerative colitis and ameliorate inflammatory damage. The mechanism of action of Rosa roxburghii Radix aqueous decoction intervention in ulcerative colitis may be related to the inhibition of TLR4/NF-κB signaling pathway.
  • [1]
    ORDÁS I, ECKMANN L, TALAMINI M, et al. Ulcerative colitis[J]. Lancet,2012,380(9853):1606−1619. doi: 10.1016/S0140-6736(12)60150-0
    [2]
    MAK W Y, ZHAO M, NG S C, et al. The epidemiology of inflammatory bowel disease: East meets west[J]. J Gastroenterol Hepatol,2020,35(3):380−389. doi: 10.1111/jgh.14872
    [3]
    EISENSTEIN M. Ulcerative colitis: Towards remission[J]. Nature,2018,563(7730):S33. doi: 10.1038/d41586-018-07276-2
    [4]
    DE SOUZA H S, FIOCCHI C. Immunopathogenesis of IBD: Current state of the art[J]. Nat Rev Gastroenterol Hepatol,2016,13(1):13−27. doi: 10.1038/nrgastro.2015.186
    [5]
    CROWELL K T , MESSARIS E. Ulcerative colitis[J]. Nat Rev Dis Primers, 2020, 6(1): 73.
    [6]
    ZHOU M, XU W, WANG J, et al. Boosting mTOR-dependent autophagy via upstream TLR4-MyD88-MAPK signalling and downstream NF-κB pathway quenches intestinal inflammation and oxidative stress injury[J]. EBio Medicine,2018,35:345−360. doi: 10.1016/j.ebiom.2018.08.035
    [7]
    KE X, ZHOU F, GAO Y, et al. Qing Hua Chang Yin exerts therapeutic effects against ulcerative colitis through the inhibition of the TLR4/NF-κB pathway[J]. Int J Mol Med,2013,32(4):926−930. doi: 10.3892/ijmm.2013.1458
    [8]
    梁勇, 李良群, 王丽, 等. 民族药刺梨根茎化学成分及其抗炎活性研究[J/OL]. 广西植物: 1−12 [2021-12-06]. http://kns.cnki.net/kcms/detail/45.1134.Q.20210929.1821.007.html.

    LIANG Yong, LI Liangqun, WANG Li, et al. Chemical constituents and their anti-inflammatory activities from rhizome of ethnicmedicine Rosa roxbunghii[J]. Guihaia: 1−12 [2021-12-06] . http://kns.cnki.net/kcms/detail/45.1134.Q.20210929.1821.007.html.
    [9]
    陈建中, 蒙启飞, 陈建华, 等. 刺梨根煎液防治慢性胃溃疡的实验研究[J]. 贵州医药,2001,25(7):584−585. [CHEN Jianzhong, MENG Qifei, CHEN Jianhua, et al. The experiment study of chronic gastric ulcer treatment with root of Rose roxburghii Tratt in rats[J]. Guizhou Medical Journal,2001,25(7):584−585. doi: 10.3969/j.issn.1000-744X.2001.07.003

    CHEN Jianzhong, MENG Qifei, CHEN Jianhua, et al. The experiment study of chronic gastric ulcer treatment with root of Rose roxburghii Tratt in rats[J]. Guizhou Medical Journal, 2001, 25(7): 584-585. doi: 10.3969/j.issn.1000-744X.2001.07.003
    [10]
    陈云志, 刘安英. 鲜刺梨根煎剂治疗急性细菌性痢疾52例[J]. 新中医,2007,39(7):70. [CHEN Yunzhi, LIU Anying. 52 cases of acute bacterial dysentery treated with fresh ethnicmedicine Rosa roxbunghii decoction[J]. Journal of New Chinese Medicine,2007,39(7):70. doi: 10.3969/j.issn.0256-7415.2007.07.044

    CHEN Yunzhi, LIU Anying. 52 cases of acute bacterial dysentery treated with fresh ethnicmedicine Rosa roxbunghii decoction[J]. Journal of New Chinese Medicine, 2007, 39(7): 70. doi: 10.3969/j.issn.0256-7415.2007.07.044
    [11]
    杨显娟, 付尹, 王佳俊, 等. 黄连-厚朴配伍抑制PI3K/Akt信号通路改善TNBS诱导的大鼠溃疡性结肠炎研究[J]. 中草药,2021,52(15):4587−4597. [YANG Xianjuan, FU Yin, WANG Jiajun, et al. Effect of coptidis rhizoma-magnoliae officinalis cortex on TNBS-induced ulcerative colitis in rats by inhibiting PI3K/Akt signaling pathway[J]. Chinese Traditional and Herbal Drugs,2021,52(15):4587−4597. doi: 10.7501/j.issn.0253-2670.2021.15.017

    YANG Xianjuan, FU Yin, WANG Jiajun, et al. Effect of coptidis rhizoma-magnoliae officinalis cortex on TNBS-induced ulcerative colitis in rats by inhibiting PI3K/Akt signaling pathway[J]. Chinese Traditional and Herbal Drugs, 2021, 52(15): 4587-4597. doi: 10.7501/j.issn.0253-2670.2021.15.017
    [12]
    吴东升, 曹晖, 张彧, 等. 芍药汤对溃疡性结肠炎大鼠肠黏膜免疫屏障的干预作用[J]. 中国实验方剂学杂志,2018,25(9):6−11. [WU Dongsheng, CAO Hui, ZHANG Yu, et al. Intervention effect of Shaoyaotang on intestinal mucosal lmmune barrier in rats with ulcerative colitis[J]. Chinese Journal of Experimental Traditional Medical Formulae,2018,25(9):6−11.

    WU Dongsheng, CAO Hui, ZHANG Yu, et al. Intervention effect of Shaoyaotang on intestinal mucosal lmmune barrier in rats with ulcerative colitis[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2018, 25(9): 6-11.
    [13]
    SUN L D, WANG F, DAI F, et al. Development and mechanism investigation of a new piperlongumine derivative as a potent anti-inflammatory agent[J]. Biochem Pharmacol,2015,95(3):156−169. doi: 10.1016/j.bcp.2015.03.014
    [14]
    KUZMICH N N, SIVAK K V, CHUBAREV V N, et al. TLR4 signaling pathway modulators as potential therapeutics in inflammation and sepsis[J]. Vaccines (Basel),2017,5(4):34. doi: 10.3390/vaccines5040034
    [15]
    ATREYA I, ATREYA R, NEURATH M F. NF-kappaB in inflammatory bowel disease[J]. J Intern Med,2008,263(6):591−596. doi: 10.1111/j.1365-2796.2008.01953.x
    [16]
    KAISHO T, AKIRA S. Toll-like receptor function and signaling[J]. J Allergy Clin Immunol,2006,117(5):979−987. doi: 10.1016/j.jaci.2006.02.023
    [17]
    CHAMANARA M, RASHIDIAN A, MEHR S E, et al. Melatonin ameliorates TNBS-induced colitis in rats through the melatonin receptors: Involvement of TLR4/MyD88/NF-κB signalling pathway[J]. Inflammopharmacology,2019,27(2):361−371. doi: 10.1007/s10787-018-0523-8
    [18]
    ZHAO R, YING M, GU S, et al. Cysteinyl leukotriene receptor 2 is involved in inflammation and neuronal damage by mediating microglia M1/M2 polarization through NF-κB pathway[J]. Neuroscience,2019,422:99−118. doi: 10.1016/j.neuroscience.2019.10.048
    [19]
    MIYAKE K. Endotoxin recognition molecules MD-2 and toll-like receptor 4 as potential targets for therapeutic intervention of endotoxin shock[J]. Curr Drug Targets Inflamm Allergy,2004,3(3):291−297. doi: 10.2174/1568010043343633
    [20]
    INDARAM A V, VISVALINGAM V, LOCKE M, et al. Mucosal cytokine production in radiation-induced proctosigmoiditis compared with inflammatory bowel disease[J]. Am J Gastroenterol,2000,95(5):1221−1225. doi: 10.1111/j.1572-0241.2000.02013.x
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