Active Components, Pharmacological Action and Application in Food Processing of <i>Eucommia ulmoides</i> Leaves

GONG Pin; HAN Yewen; ZHAI Pengtao; CHEN Xuefeng; ZHAI Wenjun; ZHENG Benzhong; CHEN Fuxin

doi:10.13386/j.issn1002-0306.2021050082

Volume 43 Issue 10

May 2022

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Science and Technology of Food Industry > 2022 > 43(10): 395-404. > DOI: 10.13386/j.issn1002-0306.2021050082

GONG Pin, HAN Yewen, ZHAI Pengtao, et al. Active Components, Pharmacological Action and Application in Food Processing of Eucommia ulmoides Leaves[J]. Science and Technology of Food Industry, 2022, 43(10): 395−404. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021050082.

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Active Components, Pharmacological Action and Application in Food Processing of Eucommia ulmoides Leaves

1.
College of Food and Bioengineering, Shaanxi University of Science and Technology, Xi’an 710021, China
2.
Shaanxi Preschool Normal University, Xi’an 710000, China
3.
Shaanxi Tiancheng Biotechnology Co., Ltd., Xi’an 710075, China
4.
College of Chemistry and Chemical Engineering, Xi’an University of Science and Technology, Xi’an 710054, China

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Received Date: May 11, 2021
Accepted Date: March 09, 2022
Available Online: March 18, 2022

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Abstract

Abstract

Eucommia ulmoides is rich in a large number of active ingredients. Studies prove that the leaves and bark of Eucommia ulmoides have the same pharmacological effects, but it has not been effectively developed. In this paper, the active components, pharmacological effects and application in food processing of Eucommia ulmoides leaves are reviewed, so as to lay a theoretical foundation for deep processing and high-value development of Eucommia ulmoides leaves.
- Eucommia ulmoides leaves,
- active ingredient,
- pharmacological effect,
- processing and application

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References

[1]	全熙宇, 彭湃, 文沛瑶, 等. 杜仲叶多糖、杜仲精粉、杜仲胶的提取分离及其性能分析[J]. 林产化学与工业,2019,39(2):122−128. [QUAN X Y, PENG P, WEN P Y, et al. Extraction and separation of Eucommia ulmoides leaf polysaccharides, Eucommia ulmoides powder, Eucommia ulmoides gum and their performance analysis[J]. Forest Products Chemistry and Industry,2019,39(2):122−128. doi: 10.3969/j.issn.0253-2417.2019.02.018 QUAN X Y, PENG P, WEN P Y, et al. Extraction and separation of Eucommia ulmoides leaf polysaccharides, Eucommia ulmoides powder, Eucommia ulmoides gum and their performance analysis [J]. Forest Products Chemistry and Industry, 2019, 39(2): 122-128. doi: 10.3969/j.issn.0253-2417.2019.02.018
[2]	李江月, 王建淇, 古云. 汉中市杜仲资源现状及开发利用对策[J]. 现代农业科技,2020,765(765):89−92. [LI J Y, WANG J Q, GU Y. Current situation and development and utilization countermeasures of Eucommia ulmoides in Hanzhong City[J]. Modern Agricultural Science and Technology,2020,765(765):89−92. LI J Y, WANG J Q, GU Y. Current situation and development and utilization countermeasures of Eucommia ulmoides in Hanzhong City[J]. Modern Agricultural Science and Technology, 2020, No. 765(07): 89+92.
[3]	WANG C, TANG L, HE J W, et al. Ethnobotany, phytochemistry and pharmacological properties of Eucommia ulmoides: A review[J]. The American Journal of Chinese Medicine,2019,47(2):259−300. doi: 10.1142/S0192415X19500137
[4]	DAI X, HUANG Q, ZHOU B, et al. Preparative isolation and purification of seven main antioxidants from Eucommia ulmoides Oliv. (Du-zhong) leaves using HSCCC guided by DPPH-HPLC experiment[J]. Food Chemistry,2013,139(1-4):563−570. doi: 10.1016/j.foodchem.2013.02.006
[5]	郭姝, 刘杰, 李娜, 等. 指纹图谱结合一测多评法评价杜仲叶代替杜仲皮的可行性[J]. 中药材,2020,43(4):896−902. [GUO S, LIU J, LI N, et al. Evaluation of the feasibility of replacing bark with leaves of Eucommia ulmoides by fingerprint combined with one measurement and multiple evaluation method[J]. Chinese Materia Medica,2020,43(4):896−902. GUO S, LIU J, LI N, et al. Evaluation of the feasibility of replacing bark with leaves of Eucommia ulmoides by fingerprint combined with one measurement and multiple evaluation method [J]. Chinese Materia Medica, 2020, 43(4): 896-902.
[6]	姚新生. 天然药物化学(第二版)[M]. 北京: 人民卫生出版社, 1988. YAO X S. Chemistry of natural medicines (second edition) [M]. Beijing: People's Medical Publishing House, 1988.
[7]	姚丽娜. 杜仲的化学成分研究[D]. 天津: 天津大学, 2010. YAO L N. Study on the chemical composition of Eucommia ulmoides [D]. Tianjin: Tianjin University, 2010.
[8]	刘聪, 郭非非, 肖军平, 等. 杜仲不同部位化学成分及药理作用研究进展[J]. 中国中药杂志,2020,45(3):497−512. [LIU C, GUO F F, XIAO J P, et al. Research progress on chemical constituents and pharmacological effects of different parts of Eucommia ulmoides[J]. Chinese Journal of Chinese Materia Medica,2020,45(3):497−512. LIU C, GUO F F, XIAO J P, et al. Research progress on chemical constituents and pharmacological effects of different parts of Eucommia ulmoides[J]. Chinese Journal of Chinese Materia Medica, 2020, 45(3): 497-512.
[9]	王亚洁, 何玉钰. 近年杜仲茶成分及工艺探讨[J]. 科学教育,2017(7):192−192. [WANG Y J, HE Y Y. Discussion on the ingredients and technology of Eucommia tea in recent years[J]. Science Education,2017(7):192−192. WANG Y J, HE Y Y. Discussion on the ingredients and technology of Eucommia tea in recent years[J]. Science Education, 2017(7): 192-192.
[10]	龚桂珍, 宫本红, 张学俊, 等. 杜仲叶和杜仲皮中化学成分的比较[J]. 西南大学学报:自然科学,2010,32(7):167−172. [GONG G Z, GONG B H, ZHANG X J, et al. Comparison of chemical components in Eucommia ulmoides leaves and Eucommia ulmoides bark[J]. Journal of Southwest University:Natural Science,2010,32(7):167−172. GONG G Z, GONG B H, ZHANG X J, et al. Comparison of chemical components in Eucommia ulmoides leaves and Eucommia ulmoides bark[J]. Journal of Southwest University: Natural Science, 2010, 32(7): 167-172.
[11]	VANHOLME R, DEMEDTS B, MORREEL K, et al. Lignin biosynthesis and structure[J]. Plant Physiology,2010,153(3):895−905. doi: 10.1104/pp.110.155119
[12]	王娟娟, 秦雪梅, 高晓霞, 等, 杜仲化学成分、药理活性和质量控制现状研究进展[J]. 中草药, 2017, 48(15): 3228−3237. WANG J J, QIN X M, GAO X X, et al. Research progress on chemical constituents, pharmacological activity and quality control of Eucommia ulmoides[J]. Chinese Herbal Medicine, 2017, 48(15): 3228−3237.
[13]	栾庆祥. 杜仲化学成分和药理作用研究进展[J]. 安徽农业科学,2016,44(9):153−156. [LUAN Q X. Research progress on the chemical constituents and pharmacological effects of Eucommia ulmoides[J]. Anhui Agricultural Sciences,2016,44(9):153−156. doi: 10.3969/j.issn.0517-6611.2016.09.053 LUAN Q X. Research progress on the chemical constituents and pharmacological effects of Eucommia ulmoides [J]. Anhui Agricultural Sciences, 2016, 44(9): 153-156. doi: 10.3969/j.issn.0517-6611.2016.09.053
[14]	赖娟华, 徐丽瑛, 饶华, 等. 杜仲叶化学成分和药理作用研究概况[J]. 实用中西医结合临床,2004,4(2):67−68. [LAI J H, XU L Y, RAO H, et al. Overview of research on chemical constituents and pharmacological effects of Eucommia ulmoides leaves[J]. Practical Integrated Traditional Chinese and Western Medicine Clinic,2004,4(2):67−68. doi: 10.3969/j.issn.1671-4040.2004.02.069 LAI J H, XU L Y, RAO H, et al. Overview of research on chemical constituents and pharmacological effects of Eucommia ulmoides leaves[J]. Practical Integrated Traditional Chinese and Western Medicine Clinic, 2004, 4(2): 67-68. doi: 10.3969/j.issn.1671-4040.2004.02.069
[15]	李锟, 郝志友, 张翠利, 等. 杜仲化学成分研究[J]. 中药材,2016,36(9):46−48. [LI K, HAO Z Y, ZHANG C L, et al. Study on the chemical constituents of Eucommia ulmoides[J]. Chinese Medicinal Materials,2016,36(9):46−48. LI K, HAO Z Y, ZHANG C L, et al. Study on the chemical constituents of Eucommia ulmoides[J]. Chinese Medicinal Materials, 2016, 36(9): 46-48.
[16]	李健民, 徐艳明, 朱魁元, 等. 杜仲抗氧化生物活性研究进展[J]. 中医药学报,2010,38(2):137−139. [LI J M, XU Y M, ZHU K Y, et al. Research progress in antioxidative biological activity of Eucommia ulmoides[J]. Journal of Traditional Chinese Medicine,2010,38(2):137−139. doi: 10.3969/j.issn.1002-2392.2010.02.056 LI J M, XU Y M, ZHU K Y, et al. Research progress in antioxidative biological activity of Eucommia ulmoides[J]. Journal of Traditional Chinese Medicine, 2010, 38(2): 137-139. doi: 10.3969/j.issn.1002-2392.2010.02.056
[17]	左月明, 张忠立, 李于益, 等. 杜仲叶木脂素类化学成分研究[J]. 时珍国医国药,2014,25(6):1317−1319. [ZUO Y M, ZHANG Z L, LI Y Y, et al. Study on the chemical constituents of lignans in Eucommia ulmoides leaves[J]. Shizhen Traditional Chinese Medicine and Materia Medica,2014,25(6):1317−1319. ZUO Y M, ZHANG Z L, LI Y Y, et al. Study on the chemical constituents of lignans in Eucommia ulmoides leaves[J]. LI Shi-zhen Traditional Chinese Medicine and Materia Medica, 2014, 25(6): 1317-1319.
[18]	管淑玉, 苏薇薇. 杜仲化学成分与药理研究进展[J]. 中药材,2003,26(2):124−129. [GUAN S Y, SU W W. Research progress on chemical constituents and pharmacology of Eucommia ulmoides[J]. Chinese Medicinal Materials,2003,26(2):124−129. doi: 10.3321/j.issn:1001-4454.2003.02.025 GUAN S Y, SU W W. Research progress on chemical constituents and pharmacology of Eucommia ulmoides [J]. Chinese medicinal materials, 2003, 26(2): 124-129. doi: 10.3321/j.issn:1001-4454.2003.02.025
[19]	化雪艳. 杜仲菌质制备及菌质烘焙食品研制[D]. 吉首: 吉首大学, 2014. HUA X Y. Preparation of Eucommia ulmoides bacterial and development of bacterial bake food [D]. Jishou: Jishou University, 2014.
[20]	王菲菲, 张聿梅, 郑笑为, 等. 环烯醚萜类化合物的结构和生物学活性研究进展[J]. 中国药事,2019,33(3):323−330. [WANG F F, ZHANG Y M, ZHENG X W, et al. Research progress on the structure and biological activity of iridoids[J]. Chinese Pharmaceutical Affairs,2019,33(3):323−330. WANG F F, ZHANG Y M, ZHENG X W, et al. Research progress on the structure and biological activity of iridoids[J]. Chinese Pharmaceutical Affairs, 2019, 33(3): 323-330.
[21]	CHIKA, TAKAMURA, TETSUYA, et al. Iridoids from the green leaves of Eucommia ulmoides[J]. Journal of Natural Products,2007,70(8):1312−1316. doi: 10.1021/np0780046
[22]	高秀梅, 王虹, 苏艳芳, 等. 杜仲化学成分作为植物雌激素的新用途: 中国, 101647850A[P]. 2010-02-17. GAO X M, WANG H, SU Y F, et al. New uses of Eucommia ulmoides as phytoestrogens: China, 101647850A[P]. 2010-02-17.
[23]	贾智若, 朱小勇, 李兵, 等. 不同产地杜仲叶挥发油成分的GC-MS分析[J]. 中国实验方剂学杂志,2013,19(19):118−122. [ JIA Z R, ZHU X Y, LI B, et al. GC-MS analysis of volatile oil from Eucommia ulmoides leaves from different origins[J]. Chinese Journal of Experimental Formulas,2013,19(19):118−122. JIA Z R, ZHU X Y, LI B, et al. GC-MS analysis of volatile oil from Eucommia ulmoides leaves from different origins[J]. Chinese Journal of Experimental Formulas, 2013, 19(19): 118-122.
[24]	马博, 张媛, 张达义, 等. 杜仲的化学成分及其药理作用研究进展[J]. 西部中医药,2013,26(12):153−159. [MA B, ZHANG Y, ZHANG D Y, et al. Research progress on the chemical constituents and pharmacological effects of Eucommia ulmoides[J]. Western Traditional Chinese Medicine,2013,26(12):153−159. MA B, ZHANG Y, ZHANG D Y, et al. Research progress on the chemical constituents and pharmacological effects of Eucommia ulmoides[J]. Western Traditional Chinese Medicine, 2013, 26(12): 153-159.
[25]	张威鹏, 朱雯, 张良, 等. 杜仲主要活性成分含量及其与树龄和部位的相关性[J]. 经济林研究,2020,38(3):46−57. [ZHANG W P, ZHU W, ZHANG L, et al. The content of main active components of Eucommia ulmoides and its correlation with tree age and position[J]. Economic Forest Research,2020,38(3):46−57. ZHANG W P, ZHU W, ZHANG L, et al. The content of main active components of Eucommia ulmoides and its correlation with tree age and position[J]. Economic Forest Research, 2020, 38(3): 46-57.
[26]	张子东, 付冬梅, 张威鹏, 等. HPLC法同时测定不同生长年限不同部位杜仲中5种苯丙素类成分[J]. 食品科学,2019,40(8):186−191. [ZHANG Z D, FU D M, ZHANG W P, et al. Simultaneous determination of 5 phenylpropanoids in different parts of Eucommia ulmoides with different growth years by HPLC method[J]. Food Science,2019,40(8):186−191. doi: 10.7506/spkx1002-6630-20180417-231 ZHANG Z D, FU D M, ZHANG W P, et al. Simultaneous determination of 5 phenylpropanoids in different parts of Eucommia ulmoides with different growth years by HPLC method[J]. Food Science, 2019, 40(8): 186-191. doi: 10.7506/spkx1002-6630-20180417-231
[27]	王莹, 阿来·赛坎, 邢亚楠, 等. 杜仲叶中总黄酮与总多糖的含量分析[J]. 应用化工,2016,45(3):550−552. [WANG Y, ALAI·SAIKAN, XING Y N, et al. Content analysis of total flavonoids and total polysaccharides in Eucommia ulmoides leaves[J]. Applied Chemical Industry,2016,45(3):550−552. WANG Y, ALAI·SAIKAN, XING Y N, et al. Content analysis of total flavonoids and total polysaccharides in Eucommia ulmoides leaves[J]. Applied Chemical Industry, 2016, 45(3): 550-552.
[28]	陈景超, 王彦宝. 杜仲叶中黄酮不同提取方法的比较[J]. 黑龙江科技信息,2011(21):20. [CHEN J C, WANG Y B. Comparison of different extraction methods of flavonoids in Eucommia ulmoides leaves[J]. Heilongjiang Science and Technology Information,2011(21):20. CHEN J C, WANG Y B. Comparison of different extraction methods of flavonoids in Eucommia ulmoides leaves[J]. Heilongjiang Science and Technology Information, 2011(21): 20.
[29]	张威鹏, 朱雯, 张良, 等. HPLC法同时测定不同采收年限及部位杜仲中5种黄酮类成分[J]. 黑龙江医药,2020,33(6):1267−1270. [ZHANG W P, ZHU W, ZHANG L, et al. Simultaneous determination of five flavonoids in Eucommia ulmoides in different harvest years and positions by HPLC method[J]. Heilongjiang Medicine,2020,33(6):1267−1270. ZHANG W P, ZHU W, ZHANG L, et al. Simultaneous determination of five flavonoids in Eucommia ulmoides in different harvest years and positions by HPLC method[J]. Heilongjiang Medicine, 2020, 33(6): 1267-1270.
[30]	张留记, 李宁, 屠万倩, 等. HPLC法同时测定杜仲叶中8种成分的含量[J]. 中国药房,2019,30(24):3383−3387. [ZHANG L J, LI N, TU W Q, et al. Simultaneous determination of the contents of 8 components in Eucommia ulmoides leaves by HPLC[J]. China Pharmacy,2019,30(24):3383−3387. ZHANG L J, LI N, TU W Q, et al. Simultaneous determination of the contents of 8 components in Eucommia ulmoides leaves by HPLC[J]. China Pharmacy, 2019, 30(24): 3383-3387.
[31]	王亮亮, 唐小兰, 王凯, 等. 杜仲的活性成分和保健功效及杜仲在食品加工中的应用[J]. 食品安全质量检测学报,2020,11(10):3074−3080. [WANG L L, TANG X L, WANG K, et al. Eucommia ulmoides active ingredients and health effects and its application in food processing[J]. Journal of Food Safety and Quality Inspection,2020,11(10):3074−3080. WANG L L, TANG X L, WANG K, et al. Eucommia ulmoides active ingredients and health effects and its application in food processing[J]. Journal of Food Safety and Quality Inspection, 2020, 11(10): 3074-3080.
[32]	张学俊, 伊廷金, 孙黔云, 等. 杜仲叶多糖的提取分离, 抗补体活性及结构研究[J]. 天然产物研究与开发,2011,23(4):606−611. [ZHANG X J, YI T J, SUN Q Y, et al. Extraction, isolation, anti-complement activity and structure of polysaccharides from Eucommia ulmoides leaves[J]. Natural Products Research and Development,2011,23(4):606−611. doi: 10.3969/j.issn.1001-6880.2011.04.004 ZHANG X J, YI T J, SUN Q Y, et al. Extraction, isolation, anti-complement activity and structure of polysaccharides from Eucommia ulmoides Leaves [J]. Natural Products Research and Development, 2011, 23(4): 606-606. doi: 10.3969/j.issn.1001-6880.2011.04.004
[33]	陈蕾. 杜仲叶中多糖分子物的提取及其对运动人体免疫机能的影响[J]. 基因组学与应用生物学,2019,38(11):374−379. [CHEN L. Extraction of polysaccharide molecules from Eucommia ulmoides leaves and its effect on the immune function of exercise human[J]. Genomics and Applied Biology,2019,38(11):374−379. CHEN L. Extraction of polysaccharide molecules from Eucommia ulmoides leaves and its effect on the immune function of exercise human [J]. Genomics and Applied Biology, 2019, 38(11): 374-379.
[34]	邓云云, 张俊娥. 杜仲愈伤组织及其叶和皮的抗氧化活性及降血糖能力比较[J]. 分子植物育种,2019,17(3):1006−1010. [DENG Y Y, ZHANG J E. Comparison of antioxidant activity and hypoglycemic ability of Eucommia ulmoides Callus, its leaves and bark[J]. Molecular Plant Breeding,2019,17(3):1006−1010. DENG Y Y, ZHANG J E. Comparison of antioxidant activity and hypoglycemic ability of Eucommia ulmoides callus, its leaves and bark [J]. Molecular Plant Breeding, 2019, 17(3): 1006-1010.
[35]	彭密军, 彭胜, 王翔, 等. 杜仲叶中多酚类化合物含量与主要生态因子的相关性研究[J]. 天然产物研究与开发,2018,30(5):823−831. [PENG M J, PENG S, WANG X, et al. Study on the correlation between the content of polyphenols in Eucommia ulmoides leaves and the main ecological factors[J]. Research and Development of Natural Products,2018,30(5):823−831. PENG M J, PENG S, WANG X, et al. Study on the correlation between the content of polyphenols in Eucommia ulmoides leaves and the main ecological factors[J]. Research and development of natural products, 2018, 30(5): 823-831.
[36]	ZHANG X, CHENG C, ZHANG M, et al. Effect of alkali and enzymatic pretreatments of Eucommia ulmoides leaves and barks on the extraction of gutta percha[J]. Journal of Agricultural & Food Chemistry,2008,56(19):62−65.
[37]	周芳, 刘韶, 邓梦如, 等. GC-MS/SIM法测定不同产地杜仲叶中的氨基酸[J]. 中南药学,2012,10(4):257−260. [ZHOU F, LIU S, DENG M R, et al. Determination of amino acids in Eucommia ulmoides leaves by GC-MS/SIM method[J]. Zhongnan Pharmacy,2012,10(4):257−260. doi: 10.3969/j.issn.1672-2981.2012.04.006 ZHOU F, LIU S, DENG M R, et al. Determination of amino acids in Eucommia ulmoides leaves by GC-MS/SIM method[J]. Zhongnan Pharmacy, 2012, 10(4): 257-260. doi: 10.3969/j.issn.1672-2981.2012.04.006
[38]	周利兵, 蒋才云, 蓝峻峰. 不同产地杜仲叶中氨基酸的化学计量分析[J]. 安徽农业科学,2020,48(19):180−181. [ZHOU L B, JIANG C Y, LAN J F. Stoichiometric analysis of amino acids in Eucommia ulmoides leaves from different origins[J]. Anhui Agricultural Sciences,2020,48(19):180−181. doi: 10.3969/j.issn.0517-6611.2020.19.047 ZHOU L B, JIANG C Y, LAN J F. Stoichiometric analysis of amino acids in Eucommia ulmoides leaves from different origins[J]. Anhui Agricultural Sciences, 2020, 48(19): 180-181. doi: 10.3969/j.issn.0517-6611.2020.19.047
[39]	钟淑娟, 杨欣, 李静, 等. 杜仲不同部位总黄酮含量及抗氧化活性研究[J]. 中国药房,2017,28(13):1787−1790. [ZHONG S J, YANG X, LI J, et al. Study on content and antioxidant activity of total flavonoids from different parts of Eucommia ulmoides[J]. China Pharmacy,2017,28(13):1787−1790. doi: 10.6039/j.issn.1001-0408.2017.13.17 ZHONG S J, YANG X, LI J, et al. Study on content and antioxidant activity of total flavonoids from different parts of Eucommia ulmoides [J]. China Pharmacy, 2017, 28(13): 1787-1790. doi: 10.6039/j.issn.1001-0408.2017.13.17
[40]	陈辉. 杜仲及其提取物的生物学功能及其在养猪生产中的应用[J]. 广东饲料,2020,29(10):39−42. [CHEN H. The biological function of Eucommia ulmoides and its extracts and its application in pig production[J]. Guangdong Feed,2020,29(10):39−42. doi: 10.3969/j.issn.1005-8613.2020.10.010 CHEN H. The biological function of Eucommia ulmoides and its extracts and its application in pig production[J]. Guangdong Feed, 2020, 29(10): 39-42. doi: 10.3969/j.issn.1005-8613.2020.10.010
[41]	ZHANG Q, SU Y Q, ZHANG J F. Seasonal difference in antioxidant capacity and active compounds contents of Eucommia ulmoides oliver leaf[J]. Molecules,2013,18(2):44−48.
[42]	YEN G C, HSIEH C L. Antioxidant activity of extracts from Du-zhong (Eucommia ulmoides) toward various lipid peroxidation models in vitro[J]. Journal of Agricultural and Food Chemistry,1998,46(10):3952−3957. doi: 10.1021/jf9800458
[43]	刘静. 杜仲叶提取物的体外抗氧化作用[J]. 陕西教育学院学报,2007,23(2):65−67. [LIU J. Antioxidant effect of Eucommia ulmoides leaf extract in vitro[J]. Journal of Shaanxi Institute of Education,2007,23(2):65−67. LIU J. Antioxidant effect of Eucommia ulmoides leaf extract in vitro[J]. Journal of Shaanxi Institute of Education, 2007, 23(2): 65-67.
[44]	王翔, 胡凤杨, 杨秋玲, 等. 杜仲叶的营养评价及体外抗氧化活性分析[J]. 食品工业科技,2019,40(21):290−299. [WANG X, HU F Y, YANG Q L, et al. Nutritional evaluation andin vitro antioxidant activity analysis of Eucommia ulmoides leaves[J]. Food Industry Science and Technology,2019,40(21):290−299. WANG X, HU F Y, YANG Q L, et al. Nutritional evaluation and in vitro antioxidant activity analysis of Eucommia ulmoides leaves[J]. Food Industry Science and Technology, 2019, 40(21): 290-299.
[45]	杨志友, 马智慧, 邓嘉航, 等. 药食同源植物杜仲叶中有效成分京尼平苷酸的抗炎作用研究[J]. 广东化工,2020,47(22):47−48. [YANG Z Y, MA Z H, DENG J H, et al. Study on the anti-inflammatory effect of geniposide, an active ingredient in the leaves of Eucommia ulmoides, a homologous plant for medicine and food[J]. Guangdong Chemical Industry,2020,47(22):47−48. doi: 10.3969/j.issn.1007-1865.2020.22.018 YANG Z Y, MA Z H, DENG J H, et al. Study on the anti-inflammatory effect of geniposide, an active ingredient in the leaves of Eucommia ulmoides, a homologous plant for medicine and food[J]. Guangdong Chemical Industry, 2020, 47(22): 47-48. doi: 10.3969/j.issn.1007-1865.2020.22.018
[46]	韩曼飞, 张刘强, 李医明. 天然桃叶珊瑚苷及其衍生物的化学结构和药理作用研究进展[J]. 中草药,2017,48(19):4105−4113. [HAN M F, ZHANG L Q, LI Y M. Research progress on the chemical structure and pharmacological effects of natural aucubin and its derivatives[J]. Chinese Herbal Medicine,2017,48(19):4105−4113. doi: 10.7501/j.issn.0253-2670.2017.19.031 HAN M F, ZHANG L Q, LI Y M. Research progress on the chemical structure and pharmacological effects of natural aucubin and its derivatives[J]. Chinese herbal medicine, 2017, 48(19): 4105-4113. doi: 10.7501/j.issn.0253-2670.2017.19.031
[47]	王宏军, 吴国娟, 李焕荣, 等. 金银花中绿原酸提取方法的筛选及其抑菌作用[J]. 北京农学院学报,2003,18(4):262−265. [WANG H J, WU G J, LI H R, et al. Screening of extraction methods of chlorogenic acid from honeysuckle and its antibacterial effect[J]. Journal of Beijing Agricultural College,2003,18(4):262−265. doi: 10.3969/j.issn.1002-3186.2003.04.007 WANG H J, WU G J, LI H R, et al. Screening of extraction methods of chlorogenic acid from honeysuckle and its antibacterial effect[J]. Journal of Beijing Agricultural College, 2003, 18(4): 262-265. doi: 10.3969/j.issn.1002-3186.2003.04.007
[48]	万凡, 侯扶江, 伊宝, 等. 绿原酸的生理功能及其在畜禽生产中的应用[J]. 动物营养学报, 2021, 33(5): 2416-2427. WAN F, HOU F J, YI B, et al. Physiological function of chlorogenic acid and its application in livestock production. Chinese Journal of Animal Nutrition, 2021, 33(5): 2416-2427.
[49]	王红连, 张凌裳, 张东升, 等. 杜仲叶提取物对鲫鱼出血性病原菌抑菌试验的研究[J]. 饲料工业,2009,30(12):28−30. [WANG H L, ZHANG L S, ZHANG D S, et al. Study on the antibacterial test of Eucommia ulmoides leaf extract on the hemorrhagic pathogen of crucian carp[J]. Feed Industry,2009,30(12):28−30. doi: 10.3969/j.issn.1001-991X.2009.12.007 WANG H L, ZHANG L S, ZHANG D S, et al. Study on the antibacterial test of Eucommia ulmoides leaf extract on the hemorrhagic pathogen of crucian carp[J]. Feed Industry, 2009, 30(12): 28-30. doi: 10.3969/j.issn.1001-991X.2009.12.007
[50]	李旭, 刘停, 陈时建, 等. 杜仲叶绿原酸提取工艺优化及对自发性高血压大鼠的降压作用[J]. 食品科学,2013,34(14):30−34. [LI X, LIU T, CHEN S J, et al. Optimization of extraction process of chlorogenic acid from Eucommia ulmoides leaf and its antihypertensive effect on spontaneously hypertensive rats[J]. Food Science,2013,34(14):30−34. doi: 10.7506/spkx1002-6630-201314007 LI X, LIU T, CHEN S J, et al. Optimization of extraction process of chlorogenic acid from Eucommia ulmoides leaf and its antihypertensive effect on spontaneously hypertensive rats[J]. Food Science, 2013, 34(14): 30-34. doi: 10.7506/spkx1002-6630-201314007
[51]	雷燕妮, 张小斌. 杜仲叶总黄酮降血压作用的研究[J]. 陕西农业科学,2016,62(5):6−8. [LEI Y N, ZHANG X B. Research on the effect of total flavonoids of Eucommia ulmoides on lowering blood pressure[J]. Shaanxi Agricultural Sciences,2016,62(5):6−8. doi: 10.3969/j.issn.0488-5368.2016.05.002 LEI Y N, ZHANG X B. Research on the effect of total flavonoids of Eucommia ulmoides on lowering blood pressure[J]. Shaanxi Agricultural Sciences, 2016, 62(05): 6-8. doi: 10.3969/j.issn.0488-5368.2016.05.002
[52]	郎茜, 龚蕾, 叶婧, 等. 杜仲叶多糖对糖尿病大鼠的降血糖作用[J]. 现代食品科技,2020,36(10):27−32,78. [LANG Q, GONG L, YE J, et al. The effect of Eucommia ulmoides polysaccharide on reducing blood sugar in diabetic rats[J]. Modern Food Technology,2020,36(10):27−32,78. LANG Q, GONG L, YE J, et al. The effect of Eucommia ulmoides polysaccharide on reducing blood sugar in diabetic rats[J]. Modern Food Technology, 2020, 36(10): 27-32, 78.
[53]	乔红伟, 潘励山, 陈威, 等. 杜仲茶对大鼠的降血脂作用[J]. 中国比较医学杂志,2015,25(12):9−12. [QIAO H W, PAN L S, CHEN W, et al. The effect of Duzhong tea on lowering blood lipids in rats[J]. Chinese Journal of Comparative Medicine,2015,25(12):9−12. QIAO H W, PAN L S, CHEN W, et al. The effect of Duzhong tea on lowering blood lipids in rats[J]. Chinese Journal of Comparative Medicine, 2015, 25(12): 9-12.
[54]	王乙庚. 杜仲叶绿原酸和竹叶多糖降糖颗粒的研究[D]. 长沙: 中南林业科技大学, 2015. WANG Y G. Studies on chlorogenic acid and polysaccharides from Eucommia ulmoides leaves [D]. Changsha: Central South University of Forestry and Technology, 2015.
[55]	徐贤柱, 饶华, 蔡险峰, 等. 杜仲叶多糖提取及对小鼠免疫功能影响研究[J]. 时珍国医国药,2013,24(3):541−542. [XU X Z, RAO H, CAI X F, et al. Extraction of polysaccharides from Eucommia ulmoides leaves and its effect on the immune function of mice[J]. Shizhen Traditional Chinese Medicine and Materia Medica,2013,24(3):541−542. doi: 10.3969/j.issn.1008-0805.2013.03.011 XU X Z, RAO H, CAI X F, et al. Extraction of polysaccharides from Eucommia ulmoides leaves and its effect on the immune function of mice[J]. Shizhen Traditional Chinese Medicine and Materia Medica, 2013, 24(03): 541-542. doi: 10.3969/j.issn.1008-0805.2013.03.011
[56]	叶颖霞, 林岚, 赵菊香, 等. 杜仲叶多糖对免疫抑制小鼠免疫功能的影响[J]. 中药材,2015,38(7):1496−1498. [YE Y X, LIN L, ZHAO J X, et al. The effect of Eucommia ulmoides leaf polysaccharides on the immune function of immunosuppressed mice[J]. Chinese Herbal Medicines,2015,38(7):1496−1498. YE Y X, LIN L, ZHAO J X, et al. The effect of Eucommia ulmoides leaf polysaccharides on the immune function of immunosuppressed mice[J]. Chinese herbal medicines, 2015, 38(07): 1496-1498.
[57]	YANG G, KYOUNG SEO, LEE J H, et al. Suppression of splenic lymphocyte proliferation by Eucommia ulmoides and genipin[J]. Chem Biodivers,2015,12(4):538−546. doi: 10.1002/cbdv.201400376
[58]	袁带秀, 刘梦姣, 聂红, 等. 杜仲总黄酮对铅中毒小鼠免疫器官抗氧化能力的影响[J]. 卫生研究,2016,45(4):643−647. [YUAN D X, LIU M J, NIE H, et al. The effect of total flavonoids of Eucommia ulmoides on the antioxidant capacity of the immune organs in lead poisoned mice[J]. Health Research,2016,45(4):643−647. YUAN D X, LIU M J, NIE H, et al. The effect of total flavonoids of Eucommia ulmoides on the antioxidant capacity of the immune organs in lead poisoned mice[J]. Health Research, 2016, 45(4): 643-647.
[59]	栾芳菲, 李湘洲, 黄丹, 等. 杜仲叶提取物对人结直肠癌细胞HCT116及抗血管生成活性的影响[J]. 林产化学与工业,2016,36(3):95−100. [LUAN F F, LI X Z, HUANG D, et al. Effects of Eucommia leaf extract on human colorectal cancer cell HCT116 and anti-angiogenesis activity[J]. Forest Products Chemistry and Industry,2016,36(3):95−100. doi: 10.3969/j.issn.0253-2417.2016.03.014 LUAN F F, LI X Z, HUANG D, et al. Effects of Eucommia leaf extract on human colorectal cancer cell HCT116 and anti-angiogenesis activity[J]. Forest Products Chemistry and Industry, 2016, 36(03): 95-100. doi: 10.3969/j.issn.0253-2417.2016.03.014
[60]	张胜, 李湘洲, 刘子雷, 等. 杜仲叶活性成分对结肠癌细胞增殖与凋亡的影响[J]. 中华中医药学刊,2018,36(2):284−287. [ZHANG S, LI X Z, LIU Z L, et al. Effects of Eucommia leaf active components on proliferation and apoptosis of colon cancer cells[J]. Chinese Journal of Traditional Chinese Medicine,2018,36(2):284−287. ZHANG S, LI X Z, LIU Z L, et al. Effects of Eucommia leaf active components on proliferation and apoptosis of colon cancer cells[J]. Chinese Journal of Traditional Chinese Medicine, 2018, 36(2): 284-287.
[61]	李小安, 夏前明, 王秉文, 等. 杜仲叶浸膏粉抗衰老作用的研究[J]. 西部医学,2009,21(6):904−906. [LI X A, XIA Q M, WANG B W, et al. Study on the anti-aging effect of Eucommia ulmoides extract powder[J]. Western Medicine,2009,21(6):904−906. doi: 10.3969/j.issn.1672-3511.2009.06.007 LI X A, XIA Q M, WANG B W, et al. Study on the anti-aging effect of Eucommia ulmoides extract powder[J]. Western Medicine, 2009, 21(06): 904-906. doi: 10.3969/j.issn.1672-3511.2009.06.007
[62]	赵晖. 杜仲叶药理作用研究(Ⅰ)——抗衰老作用[J]. 国外医学(中医中药分册),2000(3):151−153,144.
[63]	LI Y, METORI K, KOIKE K, et al. Improvement in the turnover rate of the stratum corneum in false aged model rats by the administration of geniposidic acid in Eucommia ulmoides oliver leaf[J]. Biological & Pharmaceutical Bulletin,1999,22(6):528−585.
[64]	张缓. 杜仲抗皮肤衰老活性成分的筛选及其作用机制研究[D]. 开封: 河南大学, 2016. ZHANG H. Screening of anti-aging active ingredients of Eucommia ulmoides and its mechanism of action [D]. Kaifeng: Henan University, 2016.
[65]	戴鹏, 邓鸣涛, 张立超, 等. 杜仲叶对去势骨质疏松大鼠骨代谢的影响[J]. 中国骨质疏松杂志,2012,18(12):1127−1130. [DAI P, DENG M T, ZHANG L C, et al. Effects of Eucommia ulmoides leaves on bone metabolism in ovariectomized osteoporotic rats[J]. Chinese Journal of Osteoporosis,2012,18(12):1127−1130. doi: 10.3969/j.issn.1006-7108.2012.12.015 DAI P, DENG M T, ZHANG L C, et al. Effects of Eucommia ulmoides leaves on bone metabolism in ovariectomized osteoporotic rats[J]. Chinese Journal of Osteoporosis, 2012, 18(12): 1127-1130. doi: 10.3969/j.issn.1006-7108.2012.12.015
[66]	饶华, 徐贤柱, 王曼莹. 杜仲叶总提取物治疗去势大鼠骨质疏松症的实验研究[J]. 江西医药,2014,49(2):100−102. [RAO H, XU X Z, WANG M Y. Experimental study of the total extract of Eucommia ulmoides in the treatment of osteoporosis in ovariectomized rats[J]. Jiangxi Medicine,2014,49(2):100−102. doi: 10.3969/j.issn.1006-2238.2014.02.003 RAO H, XU X Z, WANG M Y. Experimental study of the total extract of Eucommia ulmoides in the treatment of osteoporosis in ovariectomized rats[J]. Jiangxi Medicine, 2014, 49(2): 100-102. doi: 10.3969/j.issn.1006-2238.2014.02.003
[67]	刘迪, 尚华, 宋晓宇. 杜仲叶树脂分离纯化产物的抗疲劳功效[J]. 食品科学,2013,34(5):251−254. [LIU D, SHANG H, SONG X Y. Anti-fatigue effect of Eucommia ulmoides leaf resin separation and purification product[J]. Food Science,2013,34(5):251−254. LIU D, SHANG H, SONG X Y. Anti-fatigue effect of Eucommia leaf resin separation and purification product[J]. Food Science, 2013, 34(5): 251-254.
[68]	吕锦芳, 万文琴, 宁康健. 杜仲不同部位对兔离体子宫运动性能的影响[J]. 中国中医药科技,2004,11(5):292−293. [LV J F, WAN W Q, NING K J. The effect of different parts of Eucommia ulmoides on the motility of isolated rabbit uterus[J]. China Traditional Chinese Medicine Science and Technology,2004,11(5):292−293. doi: 10.3969/j.issn.1005-7072.2004.05.018 LV J F, WAN W Q, NING K J. The effect of different parts of Eucommia ulmoides on the motility of isolated rabbit uterus[J]. China Traditional Chinese Medicine Science and Technology, 2004, 11(5): 292-293. doi: 10.3969/j.issn.1005-7072.2004.05.018
[69]	刘静. 杜仲叶提取物对力竭运动及恢复小鼠肝脏和骨骼肌的保护作用[J]. 安徽农业科学,2012,40(18):9651−9652. [LIU J. The protective effect of Du-zhong leaf extract on exhaustive exercise and recovery of liver and skeletal muscle in mice[J]. Anhui Agricultural Sciences,2012,40(18):9651−9652. doi: 10.3969/j.issn.0517-6611.2012.18.029 LIU J. The protective effect of Du-zhong leaf extract on exhaustive exercise and recovery of liver and skeletal muscle in mice[J]. Anhui Agricultural Sciences, 2012, 40(18): 9651-9652. doi: 10.3969/j.issn.0517-6611.2012.18.029
[70]	黄友谊, 冀志霞, 杨坚. 杜仲茶开发现状[J]. 茶叶机械杂志,2001(2):22−24. [HUANG Y Y, JI Z X, YANG J. Development status of Du-zhong tea[J]. Tea Machinery Magazine,2001(2):22−24. HUANG Y Y, JI Z X, YANG J. Development status of Du-zhong tea[J]. Tea Machinery Magazine, 2001(2): 22-24.
[71]	陈斌, 程小东, 唐祖志. 杜仲的功能作用及其在食品中的应用前景[J]. 现代食品科技,1999(4):19−21. [CHEN B, CHENG X D, TANG Z Z. The functional role of Eucommia ulmoides and its application prospects in food[J]. Modern Food Science and Technology,1999(4):19−21. CHEN B, CHENG X D, TANG Z Z. The functional role of Eucommia ulmoides and its application prospects in food[J]. Modern Food Science and Technology, 1999(4): 19-21.
[72]	岳红, 胡小玲, 管萍, 等. 杜仲、核桃保健型复合饮料的研制[J]. 林业科技,2001,26(1):58−60. [YUE H, HU X L, GUAN P, et al. Development of Eucommia and walnut health-care compound beverage[J]. Forestry Science and Technology,2001,26(1):58−60. doi: 10.3969/j.issn.1001-9499.2001.01.022 YUE H, HU X L, GUAN P, et al. Development of Eucommia and walnut health-care compound beverage[J]. Forestry Science and Technology, 2001, 26(1): 58-60. doi: 10.3969/j.issn.1001-9499.2001.01.022
[73]	叶文峰, 楮维元, 席银华, 等. 杜仲叶复合保健饮料的研制[J]. 食品科学,2004,25(11):446−448. [YE W F, CHU W Y, XI Y H, et al. Development of Eucommia ulmoides compound health drink[J]. Food Science,2004,25(11):446−448. doi: 10.3321/j.issn:1002-6630.2004.11.118 YE W F, CHU W Y, XI Y H, et al. Development of Eucommia ulmoides compound health drink[J]. Food Science, 2004, 25(11): 446-448. doi: 10.3321/j.issn:1002-6630.2004.11.118
[74]	张志健, 李新生, 李建育. 杜仲叶饮料加工工艺研究[J]. 食品科技,2007,32(3):183−186. [ZHANG Z J, LI X S, LI J Y. Research on Eucommia leaf beverage processing technology[J]. Food Science and Technology,2007,32(3):183−186. doi: 10.3969/j.issn.1005-9989.2007.03.051 ZHANG Z J, LI X S, LI J Y. Research on Eucommia leaf beverage processing technology[J]. Food Science and Technology, 2007, 32(3): 183-186. doi: 10.3969/j.issn.1005-9989.2007.03.051
[75]	刘亮, 卢琪, 段加彩, 等. 杜仲茶酸奶的研制及茶粉、绿原酸对酸奶品质的影响[J]. 食品科学,2010,31(12):114−118. [LIU L, LU Q, DUAN J C, et al. The development of Eucommia tea yogurt and the effects of tea powder and chlorogenic acid on the quality of yogurt[J]. Food Science,2010,31(12):114−118. LIU L, LU Q, DUAN J C, et al. The development of Eucommia tea yogurt and the effects of tea powder and chlorogenic acid on the quality of yogurt[J]. Food Science, 2010, 31(12): 114-118.