Classification and Selection of Deoxyribozymes and Its Applications

FAN Yunting; LIN Xiaojun; ZHENG Jiang; BO Jun; HUANG Jiangyuan

doi:10.13386/j.issn1002-0306.2021090123

Volume 44 Issue 1

Dec. 2022

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Science and Technology of Food Industry > 2023 > 44(1): 413-419. > DOI: 10.13386/j.issn1002-0306.2021090123

FAN Yunting, LIN Xiaojun, ZHENG Jiang, et al. Classification and Selection of Deoxyribozymes and Its Applications[J]. Science and Technology of Food Industry, 2023, 44(1): 413−419. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021090123.

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Classification and Selection of Deoxyribozymes and Its Applications

FAN Yunting^{1, 2, 3,},
LIN Xiaojun¹,
ZHENG Jiang^{1, 2, ,},
BO Jun⁴,
HUANG Jiangyuan^{1, 2}

1.
Fisheries College, Jimei University, Xiamen 361021, China
2.
Engineering Research Center of the Modern Technology for Eel Industry, Ministry of Education, Xiamen 361021, China
3.
Fujian Provincial Key Laboratory of Special Aquatic Compound Feed, Fujian Tianma Technology Group Co., Ltd., Fuqing 350308, China
4.
Third Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, China

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Received Date: September 09, 2021
Available Online: October 25, 2022

Graphical Abstract

Abstract

Abstract

Deoxyribozyme is a kind of DNA molecule with catalytic activity. It can be selected by systematic evolution of ligands by exponential enrichment (SELEX). This review focuses on the classification, selection and main applications of deoxyribozymes. According to the catalysis, deoxyribozymes can be classified into cleaved, ligated, phosphorylated and other types. Nowadays the cleaved and ligated types account for a large proportion, and nucleic acids are the main substrates of deoxyribozymes. Biotin-streptavidin method or polyacrylamide gel electrophoresis is often used to separate the catalytic DNA in selection of deoxyribozymes. Deoxyribozymes have good application prospect in metal ion detection, gene therapy and DNA molecular encryption system.
- deoxyribozyme,
- classification,
- selection,
- systematic evolution of ligands by exponential enrichment(SELEX)

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References (51)

References

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