The Optimization of the Preparation Process of Antioxidative Active Peptide from the Enzymatic Hydrolysate of Pacific Saury (<i>Cololabis saira</i>) by Response Surface Methodology

LUAN Junjia; ZHANG Shangyue; LI Angda; LI Xuepeng; LI Jianrong; LIN Hong; WANG Mingli; GUO Xiaohua; YU Jianyang; ZHOU Xiaomin

doi:10.13386/j.issn1002-0306.2021070133

Volume 43 Issue 5

Feb. 2022

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Science and Technology of Food Industry > 2022 > 43(5): 172-181. > DOI: 10.13386/j.issn1002-0306.2021070133

LUAN Junjia, ZHANG Shangyue, LI Angda, et al. The Optimization of the Preparation Process of Antioxidative Active Peptide from the Enzymatic Hydrolysate of Pacific Saury (Cololabis saira) by Response Surface Methodology[J]. Science and Technology of Food Industry, 2022, 43(5): 172−181. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021070133.

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The Optimization of the Preparation Process of Antioxidative Active Peptide from the Enzymatic Hydrolysate of Pacific Saury (Cololabis saira) by Response Surface Methodology

1.
College of Food Science and Engineering, Bohai University, National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products; National R & D Branch Center of Surimi and Surimi Products Processing, Jinzhou 121013, China
2.
Department of Navigation, PLA Dalian Naval Academy, Dalian 116018, China
3.
College of Food Science and Technology, Ocean University of China, Qingdao 266100, China
4.
Penglai Jinglu Fishery Co., Ltd., Yantai 265600, China
5.
Shandong Meijia Group Co., Ltd., Rizhao 276815, China
6.
Rongcheng Taixiang Food Products Co., Ltd., Weihai 264300, China
7.
Zhejiang Xingye Industrial Group Co., Ltd., Zhoushan 316120, China

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Received Date: July 12, 2021
Available Online: December 30, 2021

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Abstract

Abstract

The antioxidative peptides were prepared from the muscle of Pacific saury by enzymatic hydrolysis. Based on the degree of hydrolysis(DH), the content of TCA-soluble peptide, DPPH free radical scavenging rate, Fe³⁺ reducing power, ·OH radical scavenging rate and O₂⁻· radical scavenging rate, the optimal protease was selected from six kinds of commercial enzyme(neutral protease, alkaline protease, flavour protease, papain, bromelain and trypsin). Single factor experiments were carried out with five factors: Solid-liquid ratio, the amount of enzyme, enzymatic hydrolysis time, temperature and pH, and three factors (solid-liquid ratio, the amount of enzyme and enzymatic hydrolysis time) were selected to optimize the response surface methodology (RSM). And the molecular weight distribution and free amino acid content of the hydrolysate were determined under the optimal conditions. The results showed that the optimal protease was neutral protease. The influence of three factors on the antioxidant activity of the enzymatic hydrolysate were the solid-liquid ratio> the amount of enzyme> enzymatic hydrolysis time. The optimal preparation processes of the antioxidative active peptide were determined as follows: the solid-liquid ratio was 1:2.67 (mass-volume ratio, g/mL), the amount of enzyme was 0.60%, enzymatic hydrolysis time was 4.04 h, temperature was 50 ℃, and pH7.0. Under these conditions, DPPH free radical scavenging rate and Fe³⁺ reducing power were 92.33% and 0.711 (20 mg/mL), respectively, which were no significant difference with the predicted value (P>0.05). It could be seen that the antioxidative peptide peptide optimized by response surface methodology had high antioxidant activity. Under the optimal conditions, 97.87% of the components with molecular weight less than 3000 Da were the main components of the hydrolysate. A total of 16 kinds of free amino acids were detected, among which essential amino acids accounted for 39.79% and antioxidant active amino acids accounted for 37.01% of the total amino acids. This study provided some reference for the utilization of high value and the development of peptides functional food of Pacific saury.
- Pacific saury (Cololabis saira),
- enzymatic hydrolysis,
- antioxidative active peptide,
- amino acid composition,
- molecular weight

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References

[1]	花传祥, 高玉珍, 朱清澄, 等. 基于耳石微结构的西北太平洋秋刀鱼(Cololabis saira)年龄与生长研究[J]. 海洋学报,2017,39(10):46−53. [HUA C X, GAO Y Z, ZHU Q C, et al. Age and growth of Pacific saury(Cololabis saira) in the Northwest Pacific Based on otoliths[J]. Acta Oceanologica Sinica,2017,39(10):46−53.
[2]	TAKASUKA A, NISHIKAWA K, KURODA H, et al. Growth variability of Pacific saury (Cololabis saira) larvae under contrasting environments across the Kuroshio axis: Survival potential of minority versus majority[J]. Fisheries Oceanography,2016,25(4):390−406. doi: 10.1111/fog.12160
[3]	石永闯, 朱清澄, 花传祥, 等. 基于海上实测的秋刀鱼舷提网沉降和提升性能研究[J]. 海洋通报,2018,37(4):459−467. [SHI Yongchuang, ZHU Qingcheng, HUA Chuanxiang, et al. Research on settlement and lifting performance of gunwale net of saberfish based on measured at sea[J]. Marine Science Bulletin,2018,37(4):459−467. doi: 10.11840/j.issn.1001-6392.2018.04.012
[4]	MORI H, TONE Y, SHIMIZU K, et al. Studies on fish scale collagen of Pacific saury(Cololabis saira)[J]. Materials Science & Engineering C,2013,33(1):174−181.
[5]	陈建文, 高建华, 钟海明, 等. 秋刀鱼蛋白酶解物的制备及氨基酸评价[J]. 食品研究与开发,2007(9):53−55. [CHEN Jianwen, GAO Jianhua, ZHONG Haiming, et al. Preparation and amino acid evaluation of saury protein hydrolysate[J]. Food Research and Development,2007(9):53−55. doi: 10.3969/j.issn.1005-6521.2007.09.017
[6]	赵谋明, 徐巨才, 刘洋, 等. 秋刀鱼制备黄嘌呤氧化酶抑制肽的工艺优化[J]. 农业工程学报,2015,31(14):291−297. [ZHAO Mouming, XU Jucai, LIU Yang, et al. Optimization of preparation process of xanthine oxidase inhibitor peptide from Cololabis saira[J]. Transactions of the Chinese Society of Agricultural Engineering,2015,31(14):291−297. doi: 10.11975/j.issn.1002-6819.2015.14.040
[7]	MADA S B, UGWU C P, ABARSHI M M. Health Promoting effects of food-derived bioactive peptides: A review[J]. International Journal of Peptide Research and Therapeutics,2020,26(2):831−848. doi: 10.1007/s10989-019-09890-8
[8]	柯义强. 发酵法制备牦牛乳酪蛋白抗氧化肽的研究[D]. 兰州: 西北民族大学, 2021. KE Yiqiang. Study on preparation of antioxidant peptides from yak cheese protein by fermentation[D]. Lanzhou: Northwest University for Nationalities, 2021.
[9]	JEMIL I, JRIDI M, NASRI R, et al. Functional, antioxidative and antibacterial properties of protein hydrolysates prepared from fish meat fermented by Bacillus subtilis A26[J]. Process Biochemistry,2014,49(6):963−972. doi: 10.1016/j.procbio.2014.03.004
[10]	ASSEM F M, ABD E M A M, KASSEM J M, et al. Proteolysis and antioxidant activity of peptic, tryptic and chymotryptic hydrolysate of cow, buffalo, goat and camel caseins[J]. International Journal of Dairy Technology,2018,71(1):236−242. doi: 10.1111/1471-0307.12400
[11]	杨昭, 黄佳佳, 姚玉静, 等. 水解度对牡蛎酶解产物功能特性和抗氧化活性的影响[J]. 食品工业,2021,42(5):134−138. [YANG Zhao, HUANG Jiajia, YAO Yujing, et al. Effect of degree of hydrolysis on functional properties and antioxidative activity of oyster hydrolysates[J]. Food Industry,2021,42(5):134−138.
[12]	张晓頔, 张益奇, 朱凯, 等. 三文鱼皮四种蛋白酶酶解物抗氧化活性及功能特性的差异研究[J]. 核农学报,2021,35(6):1402−1409. [ZHANG Xiaodi, ZHANG Yiqi, ZHU Kai, et al. Study on differences in antioxidative activity and functional properties of four protease hydrolysates from salmon skin[J]. Acta Nuclear Agriculture,2021,35(6):1402−1409. doi: 10.11869/j.issn.100-8551.2021.06.1402
[13]	DENG Zhao, CUI Chenbin, WANG Yanan, et al. FSGHF3 and peptides, prepared from fish skin gelatin, exert a protective effect on DSS-induced colitis via the Nrf2 pathway[J]. Food & Function,2020,11(1):414−423.
[14]	CHI Changfeng, WANG Bin, WANG Yumei, et al. Isolation and characterization of three antioxidative peptides from protein hydrolysate of bluefin leatherjacket (Navodon septentrionalis) heads[J]. Journal of Functional Foods,2015,12:1−10. doi: 10.1016/j.jff.2014.10.027
[15]	朱均旺, 鞠兴荣, 王立峰, 等. 超声波辅助分步酶解法制备菜籽肽及复合酶筛选[J]. 食品科学,2010,31(13):244−248. [ZHU Junwang, JU Xingrong, WANG Lifeng, et al. Preparation of rapeseed peptides by ultrasonic-assisted stepwise enzymatic hydrolysis and screening of complex enzymes[J]. Food Science,2010,31(13):244−248.
[16]	XIE Zhengjun, HUANG Junrong, XU Xueming, et al. Antioxidative activity of peptides isolated from alfalfa leaf protein hydrolysate[J]. Food Chemistry,2008,111(2):370−376. doi: 10.1016/j.foodchem.2008.03.078
[17]	LI Wenfeng, WANG Xu, ZHANG Jing, et al. Multivariate analysis illuminates the effects of vacuum drying on the extractable and nonextractable polyphenols profile of loquat fruit[J]. Journal of Food Science,2019,84(4):726−737. doi: 10.1111/1750-3841.14500
[18]	陈秋娟, 谢微, 罗杨合. 马蹄皮提取液抗氧化的稳定性研究[J]. 食品研究与开发,2017,38(11):9−12. [CHEN Qiujuan, XIE Wei, LUO Yanghe. Study on antioxidative stability of horseshoe bark extract[J]. Food Research and Development,2017,38(11):9−12. doi: 10.3969/j.issn.1005-6521.2017.11.003
[19]	宋春艳, 李彦林, 张蔚, 等. 源于啤酒糟的阿魏酰低聚糖组成及其抗氧化活性分析[J]. 食品工业科技,2019,40(9):1−5,12. [SONG Chunyan, LI Yanlin, ZHANG Wei, et al. Composition and antioxidative activity analysis of feruloyl oligosaccharides derived from beer grains[J]. Science and Technology of Food Industry,2019,40(9):1−5,12.
[20]	陈剑岚. 氨基酸自动分析法测定养殖与野生草鱼肉的滋味成分[J]. 现代食品,2020(10):162−163. [CHEN Jianlan. Determination of flavor components of cultured and wild fish by automatic amino acid analysis[J]. Modern Food,2020(10):162−163.
[21]	叶旭昌, 刘瑜, 朱清澄, 等. 北太平洋公海秋刀鱼生物学特性初步研究[J]. 上海水产大学学报,2007(3):264−268. [YE Xuchang, LIU Yu, ZHU Qingcheng, et al. Preliminary study on the biological characteristics of Cololabis saira in the North Pacific Ocean[J]. Journal of Shanghai Fisheries University,2007(3):264−268.
[22]	徐永霞, 曲诗瑶, 赵洪雷, 等. 鳀鱼蒸煮液及酶解液的风味特性分析[J]. 现代食品科技,2019,35(12):267−275. [XU Yongxia, QU Shiyao, ZHAO Honglei, et al. Flavor characteristics analysis of anchovy cooking liquid and enzymatic hydrolysate[J]. Modern Food Science and Technology,2019,35(12):267−275.
[23]	李俊江, 潘道东, 郭宇星, 等. 鹅肉蛋白酶解条件优化及酶解产物抗氧化活性研究[J]. 食品科学,2012,33(3):126−130. [LI Junjiang, PAN Daodong, GUO Yuxing, et al. Optimization of proteolysis conditions of goose meat and study on the antioxidative activity of enzymatic hydrolysis products[J]. Food Science,2012,33(3):126−130. doi: 10.7506/spkx1002-6630-201703027
[24]	任海伟, 石菊芬, 蔡亚玲, 等. 响应面法优化超声辅助酶解制备藏系羊胎盘肽工艺及抗氧化能力分析[J]. 食品科学,2019,40(24):265−273. [REN Haiwei, SHI Jufen, CAI Yaling, et al. Optimization of ultrasound-assisted enzymatic hydrolysis for preparation of Tibetan sheep placental peptides by response surface methodology and analysis of antioxidative capacity[J]. Food Science,2019,40(24):265−273. doi: 10.7506/spkx1002-6630-20181101-009
[25]	贺雪华, 李林, 白登荣, 等. 改进型城口腊肉贮藏过程中的品质变化及货架期预测[J]. 食品科学,2017,38(11):249−255. [HE Xuehua, LI Lin, BAI Dengrong, et al. Quality change and shelf life prediction of improved Chengkou bacon during storage[J]. Food Science,2017,38(11):249−255. doi: 10.7506/spkx1002-6630-201711040
[26]	WU Wenjia, ZHANG Mengmeng, SUN Chongzhen, et al. Enzymatic preparation of immunomodulatory hydrolysates from defatted wheat germ (Triticum vulgare) globulin[J]. International Journal of Food Science & Technology,2016,51(12):2556−2566.
[27]	夏吉安, 黄凯, 李森, 等. 绿豆抗氧化肽的酶法制备及其抗氧化活性[J]. 食品与生物技术学报,2020,39(10):40−47. [XIA Ji'an, HUANG Kai, LI Sen, et al. Enzymatic preparation of mung bean antioxidative peptide and its antioxidative activity[J]. Journal of Food and Biotechnology,2020,39(10):40−47. doi: 10.3969/j.issn.1673-1689.2020.10.006
[28]	赵谋明, 刘洋, 张佳男, 等. 木糖-草鱼肽美拉德反应产物的抗氧化性[J]. 农业工程学报,2014,30(9):279−286. [ZHAO Mouming, LIU Yang, ZHANG Jianan, et al. Antioxidative properties of Maillard reaction products of xylose-grass peptide[J]. Transactions of the Chinese Society of Agricultural Engineering,2014,30(9):279−286. doi: 10.3969/j.issn.1002-6819.2014.09.035
[29]	宁诗文, 崔珊珊, 尚宏丽. 响应面法优化大黄花鱼肉蛋白水解工艺及多肽抗氧化性研究[J]. 食品工业科技, 2020, 41(13): 219 226 220-225. NING Shiwen, CUI Shanshan, SHANG Hongli. Optimization of the protein hydrolysis process of large yellow croaker fish meat and the antioxidative activity of peptides by response surface methodology[J]. Food Industry Science and Technology, 2020, 41(13): 219 226 220-225.
[30]	王倩. 蜂胶黄酮对小鼠铅中毒的保护作用及其机制研究[D]. 西安: 西北大学, 2020. WANG Qian. Protective effect of propolis flavonoids on lead poisoning in mice and its mechanism[D]. Xi'an: Northwest University, 2020.
[31]	王志, 赵峰, 王珊珊, 等. 大菱鲆鱼皮抗氧化胶原肽的制备及特性分析[J]. 食品安全质量检测学报,2020,11(22):8267−8275. [WANG Zhi, ZHAO Feng, WANG Shanshan, et al. Preparation and characteristics analysis of turbot skin antioxidative collagen peptide[J]. Journal of Food Safety and Quality Testing,2020,11(22):8267−8275.
[32]	高原. 海洋乳酸菌抗氧化活性研究[D]. 大连: 大连工业大学, 2016. GAO Yuan. Research on the antioxidative activity of marine lactic acid bacteria[D]. Dalian: Dalian University of Technology, 2016.
[33]	黄典, 高雅, 刘蕾, 等. 蛋白酶水解美味牛肝菌工艺优化及酶解产物的抗氧化活性[J]. 食品工业科技,2021,42(12):209−217. [HUANG Dian, GAO Ya, LIU Lei, et al. Process optimization of protease hydrolysis of Boletus edulis and antioxidative activity of enzymatic hydrolysis products[J]. Science and Technology of Food Industry,2021,42(12):209−217.
[34]	綦蕾, 王振宇. 红松松仁抗氧化肽的制备及体外抗氧化活性评价[J]. 食品与发酵工业,2010,36(7):78−82. [QI Lei, WANG Zhenyu. Preparation of Korean pine nuts antioxidative peptides and evaluation of antioxidative activity in vitro[J]. Food and Fermentation Industries,2010,36(7):78−82.
[35]	FLORENCIA C, ESTEBAN S J, ROSA A M, et al. Anti-inflammatory and antioxidative activities, functional properties and mutagenicity studies of protein and protein hydrolysate obtained from Prosopis alba seed flour[J]. Food Chemistry,2014,161:391−399. doi: 10.1016/j.foodchem.2014.04.003
[36]	YU Fangmiao, ZONG Chuhong, JIN Shujie, et al. Optimization of extraction conditions and characterization of pepsin-solubilised collagen from skin of giant croaker(Nibea japonica)[J]. Marine Drugs,2018,16(1):29−40. doi: 10.3390/md16010029
[37]	艾丽奇. 鳕鱼皮胶原蛋白肽的抗皮肤光老化功效及其作用机制研究[D]. 广州: 华南理工大学, 2020. AI Liqi. A study on the anti-aging effect of cod skin collagen peptide and its mechanism[D]. Guangzhou: South China University of Technology, 2020.
[38]	王嘉榕, 滕达, 田子罡, 等. 功能性抗氧化肽制备与机制研究进展[J]. 天然产物研究与开发,2008(2):371−375. [WANG Jiarong, TENG Da, TIAN Zigang, et al. Research progress in preparation and mechanism of functional antioxidant peptides[J]. Natural Products Research and Development,2008(2):371−375. doi: 10.3969/j.issn.1001-6880.2008.02.045

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