Safety Evaluation and Probiotic Characteristics Analysis of <i>Lactobacillus plantarum</i> HCS03-001

JIAO Yanping; YU Ping; ZHAO Di; ZHANG Chunyu; SONG Jia; TANG Chun; CAO Lan

doi:10.13386/j.issn1002-0306.2021060278

Volume 43 Issue 5

Feb. 2022

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Science and Technology of Food Industry > 2022 > 43(5): 165-171. > DOI: 10.13386/j.issn1002-0306.2021060278

JIAO Yanping, YU Ping, ZHAO Di, et al. Safety Evaluation and Probiotic Characteristics Analysis of Lactobacillus plantarum HCS03-001[J]. Science and Technology of Food Industry, 2022, 43(5): 165−171. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021060278.

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Safety Evaluation and Probiotic Characteristics Analysis of Lactobacillus plantarum HCS03-001

Jiangxi Renren Health Industry Co., Ltd., Zhangshu 331200, China

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Received Date: July 01, 2021
Available Online: December 27, 2021

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Abstract

Abstract

In order to evaluate the safety and probiotic properties of Lactobacillus plantarum HCS03-001(CGMCC No.16258), the safety of Lactobacillus plantarum HCS03-001 was evaluated by hemolysis test, drug resistance test and acute transoral toxicity test. The probiotic properties of the strain were evaluated by acid and bile salt tolerance test, bacteriostatic functional properties test and laxative efficacy test. The results showed that Lactobacillus plantarum HCS03-001 did not form transparent circle on the Colombian blood finger culture medium, that was, there was no hemolysis. Lactobacillus plantarum HCS03-001 was sensitive to penicillin (PEN), ampicillin (AM), imipenem (IP), meropenem (MP), linezolid (LZ), and datoramycin (DPC), and neutral to erythromycin (EM) and resistant to vancomycin (VA). The acute oral toxicology of mice was non-toxic, and the strain was confirmed to be safe to eat.The survival rate of the strain was 94.29% and 92.33% at pH3.0 and pH2.0 for 3 h, respectively. The survival rate could still reach 88.82% at 1.5 g/L bile salt for 3 h. It had inhibitory effect on intestinal and oral pathogens (Porphyromonas gingivalis and Streptococcus mutans). It had a good effect on promoting defecation in constipation model of loperamide hydrochloride. Lactobacillus plantarum HCS03-001 has good safety and probiotics.
- Lactobacillus plantarum,
- hemolytic,
- drug resistance,
- acute transoral toxicity test,
- probiotic characteristics,
- safety evaluation

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References

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