Optimization of Preparation Process of Polypeptide Metal Chelates from the Scales of American red fish (<i>Sciaenops ocellatus)</i>

WAN Jingliang; WU Peng; LIN Ling; LIU Yuansen; TANG Xu; XU Chang’an

doi:10.13386/j.issn1002-0306.2021060150

Volume 43 Issue 8

Apr. 2022

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Science and Technology of Food Industry > 2022 > 43(8): 165-171. > DOI: 10.13386/j.issn1002-0306.2021060150

WAN Jingliang, WU Peng, LIN Ling, et al. Optimization of Preparation Process of Polypeptide Metal Chelates from the Scales of American red fish (Sciaenops ocellatus)[J]. Science and Technology of Food Industry, 2022, 43(8): 165−171. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021060150.

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Optimization of Preparation Process of Polypeptide Metal Chelates from the Scales of American red fish (Sciaenops ocellatus)

Third Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, China

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Received Date: June 17, 2021
Available Online: February 16, 2022

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Abstract

Abstract

In this paper, the preparation of polypeptides and the synthesis of polypeptide metal chelates from American red fish（Sciaenops ocellatus ）scales were studied and optimized. Polypeptides were prepared from the scales of American red fish. The molecular weight of the peptides was analyzed by gel chromatography. Polypeptide metal chelates were synthesized by water system method and the metal chelating rate was determined by EDTA complexometric titration method. L₉(3⁴) orthogonal test was used to optimize the processing conditions of polypeptide metal chelates. The binding of polypeptide to metal was verified by detecting polypeptide and metal chelate by infrared spectroscopy. The molecular weight of polypeptide was about 1~6 kDa by gel chromatography analysis. The preparation conditions of polypeptide zinc chelate were obtained by orthogonal experiment, that is, the mass ratio of zinc chelate was 2:1, pH5.5, temperature 55℃, time 60 min. The optimal chelating conditions were mass ratio 2:1, pH6.0, temperature 35℃ and time 55 min. Under the optimized condition, the chelate ratio of zinc and copper were 78.21% and 91.24% respectively. The infrared absorption spectra showed that Zn²⁺, Cu²⁺ and -NH₂ were successfully combined. The results showed that the chelation rate was significantly improved under the condition of this process, and it was rich in metal ions, low cost and promising market prospect, which would be conducive to the high-value application of marine fishery processing by-products and promote the technological upgrading of fishery processing industry.
- fish scales; Sciaenops ocellatus,
- polypeptide,
- polypeptide metal chelate,
- process optimization

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[1]	周龙. 珍珠蚌肉酶解液多肽锌螯合研究[D]. 杭州: 浙江农林大学, 2014. ZHOU L. Study on pearl mussel meat enzymolysis hydrolysate polypeptide chelated with zinc[D]. Hangzhou: Zhejiang A&F University, 2014.
[2]	UDECHUKWU M C, COLLINSO S A, UDENIGWE C C. Prospects of enhancing dietary zinc bioavailability with food-derived zinc-chelating peptides[J]. Food & Function,2016,7(10):4137.
[3]	许庆陵, 王玉飞, 刘浩怀, 等. 罗非鱼多肽-Ca、Cu螯合工艺及其产物的结构特征[J]. 食品科技,2015(11):108−112. [XU Q L, WANG Y F, LIU H H, et al. Chelation technics of structure characteristics of Tilapia peptide-Ca, and peptide-Cu[J]. Food Science and Technology,2015(11):108−112.
[4]	姚宝强, 刘丽. 氨基酸微量元素螯合物的研究与应用[J]. 兽药与饲料添加剂,2008,13(3):19−22. [YAO B Q, LIU L. Research and application of amino acid trace element chelate[J]. Veterinary Pharmaceuticals & Feed Additives,2008,13(3):19−22.
[5]	黄丹萍, 胡巧玲, 李奎, 等. 有机微量元素氨基酸络(螯)合物评定研究进展[J]. 饲料研究,2015(14):27−31. [HUANG D P, HU Q L, LI K, et al. Research progress on the evaluation of organic trace element amino acid complex (chelate)[J]. Feed Research,2015(14):27−31.
[6]	TZIVELAKA L A, IOANNOU E, TSIOURVAS D, et al. Collagen from the marine sponges Axinella cannabina and Suberites carnosus: Isolation and morphological, biochemical, and biophysical characterization[J]. Marine Drugs,2017,15(6):152−156. doi: 10.3390/md15060152
[7]	虞俊翔, 孙南耀, 王光然, 等. 微量元素氨基酸螯合物的生物学效价研究进展[J]. 食品科学,2015,36(23):367−371. [YU J X, SUN N Y, WANG G R, et al. Advances in bioavailability of amino acid-microelement chelates[J]. Food Science,2015,36(23):367−371. doi: 10.7506/spkx1002-6630-201523065
[8]	任帅, 徐小健, 赵思梦, 等. 氨基酸螯合物加工制备方法研究进展[J]. 科技创新导报,2017(35):71−73. [REN S, XU X J, ZHAO S M, et al. Review on the processing and preparation methods of amino acid chelate[J]. Science and Technology Innovation Herald,2017(35):71−73.
[9]	JR W R, TOMASSO J R, III D M G. Production performance and non-specific immunity of cage-raised red drum, Sciaenops ocellatus, fed soybean-based diets[J]. Aquaculture,2015,443:84−89. doi: 10.1016/j.aquaculture.2015.03.012
[10]	农业农村部渔业渔政管理局, 全国水产技术推广总站, 中国水产学会. 2019中国渔业统计年鉴[M]. 北京: 中国农业出版社, 2020: 33−35. Fisheries and Fisheries Administration, Ministry of Agriculture and Rural Affairs, National Aquatic Technology Extension Station, Chinese Fisheries Society. 2019 China fishery statistical yearbook[M]. Beijing: China Agricultural Press, 2020: 33−35.
[11]	吴琴琴, 杨丽虹, 唐旭, 等. 鱼鳞胶原蛋白提取过程中脱脂工艺的优化[J]. 食品工业科技,2011(12):273−275. [WU Q Q, YANG L H, TANG X, et al. Optimization of degrease process during extracting collagen from sea-fish scales[J]. Science and Technology of Food Industry,2011(12):273−275.
[12]	唐旭, 何建林, 徐长安, 等. 鱼鳞胶原蛋白提取过程中的脱钙工艺条件优化[J]. 食品工业科技,2011,32(6):326−328. [TANG X, HE J L, XU C A, et al. Optimization of decalcification process during extracting collagen from scale of sea fish[J]. Science and Technology of Food Industry,2011,32(6):326−328.
[13]	杨丽虹, 吴琴琴, 唐旭, 等. 木瓜蛋白酶水解海洋鱼鳞蛋白的工艺研究[J]. 现代食品科技,2011,27(12):1484−1486. [YANG L H, WU Q Q, TANG X, et al. Hydrolysis process of sea-fish scale protein catalyzed by papain[J]. Modern Food Science and Technology,2011,27(12):1484−1486.
[14]	吴胜华, 李吕木. 微量元素氨基酸螯合物合成及螯合率测定的研究[J]. 饲料工业,2008,29(16):11−12. [WU S H, LI L M. Research on compound of amino acid microelement chelate and determination of chelate ratio[J]. Feed Industry,2008,29(16):11−12. doi: 10.3969/j.issn.1001-991X.2008.16.003
[15]	王建坤, 范新宇, 张昊, 等. 基于EDTA络合滴定法测定重金属离子浓度[J]. 天津工业大学学报,2018,37(1):38−42. [WANG J K, FANG X Y, ZHANG H, et al. Determination of heavy metal ion concentration based on EDTA complexometric titration[J]. Journal of Tiangong University,2018,37(1):38−42.
[16]	郭兴峰, 魏芳, 周祥山, 等. 苦味肽的形成机理及脱苦技术研究进展[J]. 食品研究与开发,2017,38(21):207−211. [GUO X F, WEI F, ZHOU X S, et al. Review on the formation mechanism and debittering technology of bitter peptides[J]. Food Research and Development,2017,38(21):207−211. doi: 10.3969/j.issn.1005-6521.2017.21.041
[17]	KIMIRA Y, ODAIRA H, NOMURA K, et al. Collagen-derived dipeptide prolyl-hydroxyproline promotes osteogenic differentiation through Foxg1[J]. Cellular Molecular Biology Letters,2017,22(1):27. doi: 10.1186/s11658-017-0060-2
[18]	顾冰飞, 赵圆圆, 陈义勇. 杏鲍菇多糖锌螯合物的制备工艺及其抗氧化活性[J]. 食品研究与开发,2019,40(4):105−111. [GU B F, ZHAO Y Y, CHEN Y Y. Preparationn technology of Pleurotus eryngii polysaccharides zinc(Ⅱ) chelate and its antidant acitvities[J]. Food Research and Development,2019,40(4):105−111.
[19]	JIMENEZ V E, EMILIO P, CRUCES M P, et al. Study on the relationship of genotoxic and oxidative potential of a new mixed chelate copper antitumoral drug, Casiopeina II-gly (Cas II-gly) in Drosophila melanogaster[J]. Environmental Toxicology & Pharmacology,2016,48:286−293.
[20]	阳丽红. 利用金枪鱼加工副产物制备骨粉, 胶原多肽及肽钙螯合物研究[D]. 杭州: 浙江工商大学, 2020. YANG L H. Study on the preparation of bone meal, collagen peptides and peptide calcium chelate by using the by-products of tuna processing[D]. Hangzhou: Zhejiang Gongshang University, 2020.
[21]	乔月建. 小肽螯合锌的制备及对公猪生产性能、血液生理化指标的影响[D]. 邯郸: 河北工程大学, 2016. QIAO Y J. Preparation and effects on the production performance of boars, blood physiological and biochemical indexes of zinc chelated small peptides[D]. Handan: Hebei University of Engineering, 2016.
[22]	PETRAVIC V P, ZIVANOVIC M N, SIMIJONOVIC D, et al. Study of the structure, prooxidative, and cytotoxic activity of some chelate copper(II) complexes[J]. Chemical Papers,2017,71(11):1−9.
[23]	CRESWELL C J, RUNQUIST O, CAMPBELL M M. Spectral analysis of organic compounds[M]. Burgess Publishing Company, 1972.
[24]	SUBA S, VIJAYAKUMAR S, VIDHYA E, et al. Microbial mediated synthesis of ZnO nanoparticles derived from Lactobacillus spp: Characterizations, antimicrobial and biocompatibility efficiencies[J]. Sensors International,2021(10):100104.
[25]	GONG X B. Kinetic and equilibrium studies on the adsorption of Pb(II), Cd(II) and Cu(II) by rape straw[J]. Adsorption Science & Technology,2013,31(6):559−571.
[26]	彭巧云, 沈菊泉, 魏东芝, 等. 正交试验优化胶原多肽螯合钙的制备工艺[J]. 食品科学,2013,34(8):94−99. [PENG Q Y, SHEN J Q, WEI D Z, et al. Optimization of preparation of collagen polypeptide chelated calcium by orthogonal array design[J]. Food Science,2013,34(8):94−99. doi: 10.7506/spkx1002-6630-201308019
[27]	程波, 赵君华, 朱志明, 等. 微波法制备鲟鱼皮复合氨基酸螯合铜的工艺[J]. 食品研究与开发,2009,30(9):128−134. [CHEN B, ZHAO J H, ZHU Z M, et al. The synthetic technology of Cu (Ⅱ) chelated with compound amino acids hydrolyzed from farmed sturgeon's under microwave irradiation[J]. Food Research and Development,2009,30(9):128−134. doi: 10.3969/j.issn.1005-6521.2009.09.039

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