Effect Evaluation of Liupao Tea on Improving Phlegm-dampness Constitution and Mechanism of Clearing Damp on Intestinal Microbiota

HOU Can; XIAO Jie; WANG Liming; CHEN Jieyu; ZHAO Xiaoshan; FAN Yihang; LI Song; HAO Binxiu; YING Jian; ZHANG Junwei

doi:10.13386/j.issn1002-0306.2020120100

Volume 42 Issue 21

Oct. 2021

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Science and Technology of Food Industry > 2021 > 42(21): 361-369. > DOI: 10.13386/j.issn1002-0306.2020120100

HOU Can, XIAO Jie, WANG Liming, et al. Effect Evaluation of Liupao Tea on Improving Phlegm-dampness Constitution and Mechanism of Clearing Damp on Intestinal Microbiota[J]. Science and Technology of Food Industry, 2021, 42(21): 361−369. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020120100.

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Effect Evaluation of Liupao Tea on Improving Phlegm-dampness Constitution and Mechanism of Clearing Damp on Intestinal Microbiota

HOU Can^{1, 2,},
XIAO Jie^{1, 2},
WANG Liming^{1, 2},
CHEN Jieyu³,
ZHAO Xiaoshan³,
FAN Yihang^{1, 2},
LI Song⁴,
HAO Binxiu⁴,
YING Jian^{1, 2, ,},
ZHANG Junwei^5, ,

1.
Nutrition and Health Research Institute, COFCO Ltd., Beijing 102209, China
2.
Beijing Key Laboratory of Nutrition & Health and Food Safety, Beijing 102209, China
3.
School of Chinese Medicine, Southern Medicine University, Guangzhou 510515, China
4.
China Tea Technology (Beijing) Co., Ltd., Beijing 102209, China
5.
China Tea (Wuzhou) Co., Ltd., Wuzhou 543001, China

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Received Date: December 13, 2020
Available Online: August 31, 2021

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Abstract

Abstract

To investigate the efficacy of Liupao tea on regulating phlegm-dampness constitution in sub-health state and the mechanism, a randomized, controlled clinical trial of 90-day was taken using Liupao fresh and aged tea with the intervention dose of 10 g soaked in boiling water of 200 ml for 2 min, twice a day as the intervention means. Traditional Chinese medicine diagnostic indexes and blood biochemical indexes related to phlegm-dampness were recorded before and after intervention, and the change of intestinal flora was analyzed. The results indicated that the phlegm-dampness quality translation score showed a decreasing trend in the middle and end of the intervention among the fresh tea intervention group and aged tea intervention group. The ANOVA results showed the phlegm-dampness quality translation score of fresh tea intervention group had significant differences at three time points of 25, 45 and 90 d after intervention (P<0.05). The intervention of Liupao tea could improve the phlegm-dampness constitution slowly and the effect of fresh tea was slightly faster. Biochemical results showed that Liupao tea had the effect of reducing bilirubin (total bilirubin and direct bilirubin). The effect of aged tea was obvious and the difference was statistically significant (P<0.05). Intestinal flora results showed that the level of Bacterioidetes decreased to 0.49±0.17 in fresh tea intervention group and 0.48±0.35 in aged tea intervention group, while the level of Firmicutes increased to 0.42±0.23 in fresh tea intervention group and 0.45±0.08 in aged tea intervention group after intervention. The relative abundance of Bilophila increased to 0.0024±0.0013 in fresh tea intervention group and 0.0022±0.0034 in aged tea intervention group. Liupao tea changed the relative proportion of Firmicutes and Bacteroidetes in the intestinal flora, promoted the increase of Bilophila, thus Liupao tea may improve the metabolism of blood bilirubin by regulating the bile acid pathway. The relative abundance of Sutterella, Izimaplasmatales and Ruminococcus, which are related to metabolic diseases and energy metabolism, were changed during the experiment. Therefore, Liupao tea may improve the metabolic state of sub-health people by regulating intestinal microbiota.
- Liupao tea,
- phlegm-dampness constitution,
- phlegm-dampness quality translation score,
- bilirubin,
- intestinal microbiota

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References

[1]	王琦. 中医体质学2008 [M]. 北京: 人民卫生出版社, 2009. Wang Q. Traditional Chinese medicine physique science 2008[M]. Beijing: People's Sanitary Publishing Press, 2009.
[2]	王琦, 朱燕波. 中国一般人群中医体质流行病学调查—基于全国9省市21948例流行病学调查数据[J]. 中华中医药杂志,2009,24(1):7−12. [Wang Q, Zhu Y B. Epidemiological investigation of constitutional types of Chinese medicine in general population: Base on 21948 epidemiological investigation data of nine provinces in China[J]. Chinese Journal of Traditional Chinese Medicine,2009,24(1):7−12.
[3]	董静. 痰湿体质基础研究及其与代谢综合征相关性的探索[D]. 北京: 北京中医药大学, 2007. Dong J. Foundational research on phlegm-dampness constitution and correlation between phlegm-dampness constitution and metabolic syndrome[D]. Beijing: Beijing University of Chinese Medicine, 2007.
[4]	邓小敏, 唐丽丽, 陆斌. 痰湿体质人群血脂水平调查及体质相关性研究[J]. 中国中医基础医学杂志,2013,19(4):423−433. [Deng X M, Tang L L, Lu B. Research on the relevance of blood lipids and body constitution of TCM for phlegm-dampness constitution people[J]. Chinese Journal of Basic Medicine in Traditional Chinese Medicine,2013,19(4):423−433.
[5]	韩志斌. 痰湿体质与高血压前期相关性的研究[D]. 广州: 广州中医药大学, 2014. Han Z B. The research of phlegm constitution and correlation of prehypertension[D]. Guangzhou: Guangzhou University of Traditional Chinese Medicine, 2014.
[6]	姚海强, 王济, 李玲孺, 等. 痰湿体质与肿瘤的相关性探讨[J]. 环球中医药,2016,9(5):596−598. [Xao H Q, Wang J, Li L R, et al. Study on the correlation between phlegm-dampness constitution and tumor[J]. Global Traditional Chinese Medicine,2016,9(5):596−598. doi: 10.3969/j.issn.1674-1749.2016.05.021
[7]	王琦. 痰湿体质系列研究及在代谢性慢病防控中的应用[J]. 天津中医药,2020,37(1):4−8. [Wang Q. A series of studies on phlegm-dampness constitution and the application in prevention and control of chronic metabolic disease[J]. Tianjin Journal of Traditional Chinese Medicine,2020,37(1):4−8.
[8]	王侨, 顾瞻, 何华玉, 等. 代谢综合征痰湿质研究进展[J]. 陕西中医,2019,40(12):1814−1817. [Wang Q, Gu Z, He H Y, et al. Research progress in metabolic syndrome with phlegm-dampness constitution[J]. Shanxi Journal of Traditional Chinese Medicine,2019,40(12):1814−1817. doi: 10.3969/j.issn.1000-7369.2019.12.042
[9]	曾维, 徐安雯, 刘晋利, 等. 中医体质辨识在高血压慢病管理中的应用效果观察[J]. 医药界,2019,10(19):173. [Zeng W, Xu A W, Liu J L, et al. Observation on the application effect of traditional Chinese medicine constitution identification in the management of chronic hypertension[J]. Medical Circle,2019,10(19):173.
[10]	安利红, 张霞晖. 中医体质分型与妊娠期肝内胆汁淤积症的关系[J]. 中国中西医结合消化杂志,2014,22(3):143−145. [An L H, Zhang X H. Relationship between constitution of traditional Chinese medicine and intrahepatic cholestasis of pregnancy[J]. Chinese Journal of Integrated Traditional and Western Medicine of Digestion,2014,22(3):143−145. doi: 10.3969/j.issn.1671-038X.2014.03.09
[11]	郭力群, 裴晓东. 茵陈蒿汤加减治疗痰湿质、湿热质高龄妊娠期肝内胆汁淤积症孕妇的临床研究[J]. 云南中医中药杂志,2018,39(12):48−49. [Guo L Q, Pei X D. Clinical study on treatment of phlegm-dampness and damp-heat aged pregnant women with intrahepatic cholestasis by Yin Chenhao decoction[J]. Yunnan Journal of Traditional Chinese Medicine,2018,39(12):48−49. doi: 10.3969/j.issn.1007-2349.2018.12.021
[12]	吴晴鸥. 痰湿质与饮食生活习惯的相关性[J]. 中国民间疗法,2019,27(24):75−77. [Wu Q O. The correlation between phlegm-dampness and dietary habits[J]. Chinese Journal of Folk Therapy,2019,27(24):75−77.
[13]	徐平, 王琦. 体质与亚健康状态关系的研究现状[J]. 河南中医,2017,37(12):2233−2237. [Xu P, Wang Q. Research status of the relationship between constitution and sub-health status[J]. Henan Traditional Chinese Medicine,2017,37(12):2233−2237.
[14]	陈瑞妹. 146例代谢综合征体检患者中医体质分布规律及相关因素研究[J]. 亚太传统医药,2019,15(2):181−184. [Chen R M. Study on constitution distribution and related factors of TCM in 146 patients with metabolic syndrome[J]. Asia-Pacific Traditional Medicine,2019,15(2):181−184.
[15]	夏晓莉, 蔡鑫桂, 赖伟兰, 等. 代谢综合征患者中医体质分析[J]. 广州中医药大学学报,2017,34(6):793−797. [Xia X L, Cai X G, Lai W L, et al. Analysis of traditional Chinese medicine constitution of patients with metabolic syndrome[J]. Journal of Guangzhou University of Traditional Chinese Medicine,2017,34(6):793−797.
[16]	尹超群, 杨卫华, 郭洪波. 教师MS及体质辨识调查与分析[J]. 世界中西医结合杂志,2016,11(11):1587−1589. [Yin C Q, Yang W H, Guo H B. Investigation and analysis on metabolic syndrome and body constitution identification in teachers[J]. World Journal of Integrated Traditional and Western Medicine,2016,11(11):1587−1589.
[17]	梁翠梅, 孙颂歌, 胡慧. 代谢综合征中医体质分布规律及相关因素研究[J]. 世界中西医结合杂志,2016,11(10):1372−1375, 1464. [Liang C M, Sun S G, Hu H. Distribution rule of TCM constitution and relevant factors in metabolic syndrome[J]. World Journal of Integrated Traditional and Western Medicine,2016,11(10):1372−1375, 1464.
[18]	祁丽丽, 周一心, 韩振翔, 等. 代谢综合征中医体质与血脂、BMI及生活质量相关性研究[J]. 辽宁中医杂志,2015,42(7):1171−1174. [Qi L L, Zhou Y X, Han Z X, et al. Study on relationship of TCM constitution distribution and blood lipid, BMI and SF-36 in metabolic syndrome[J]. Liaoning Traditional Chinese Medicine,2015,42(7):1171−1174.
[19]	邵铁娟, 李海昌, 谢志军, 等. 基于脾主运化理论探讨脾虚湿困与肠道菌群紊乱的关系[J]. 中华中医药杂志,2014,29(12):3762−3765. [Shao T J, Li H C, Xie Z J, et al. Relationship between the syndrome of water retention due to spleen deficiency and intestinal flora disturbance based on the theory of spleen governing transportation and transformation[J]. Chinese Journal of Traditional Chinese Medicine,2014,29(12):3762−3765.
[20]	马晔琳, 钱晓玲, 杨敏春. 中老年痰湿质人群肠道菌群结构特征研究[J]. 中华全科医学,2020,18(1):113−118. [Ma Y L, Qian X L, Yang M C. Structure characteristics of intestinal flora in middle-aged and elderly people with phlegm-dampness constitution[J]. Chinese Journal of General Practice,2020,18(1):113−118.
[21]	陈瑞芳, 陈伟鸿, 马方励, 等. 扶正气组合调理偏颇体质和改善亚健康状态的临床试验[J]. 医药导报,2016,35(S1):35−38. [Chen R F, Chen W H, Ma F L, et al. The clinical trial of Fuzhegnqi to recuperate biased constitution and improve sub-health state[J]. Herald of Medicine,2016,35(S1):35−38.
[22]	肖雅, 经媛, 陈洁瑜, 等. 桂附地黄丸干预阳虚体质的血浆代谢组学[J]. 南方医科大学学报,2016,36(11):1489−1495. [Xiao Y, Jing Y, Chen J Y, et al. Plasma metabonomics of Guifu Dihuang Wan in the treatment of yang deficiency[J]. Journal of South Medicine University,2016,36(11):1489−1495. doi: 10.3969/j.issn.1673-4254.2016.11.07
[23]	周锦涛, 刘力, 周铖, 等. 健脾祛湿法对肠道菌群结构影响的研究概述[J]. 中医药导报,2020,26(11):93−97, 130. [Zhou J T, Liu L, Zhou C, et al. Summary of research on the effect of invigorating the spleen and eliminating dampness on the structure of intestinal flora[J]. Guiding Journal of Traditional Chinese Medicine and Pharmacology,2020,26(11):93−97, 130.
[24]	包晓丹, 周兰英. 健脾化痰祛脂治疗非酒精性脂肪肝30例[J]. 河北中医,2007,29(2):127−128. [Bao X D, Zhou L Y. Treatment of 30 cases of nonalcoholic fatty liver disease by invigorating spleen, resolving phlegm and removing fat[J]. Hebei Journal of Traditional Chinese Medicine,2007,29(2):127−128. doi: 10.3969/j.issn.1002-2619.2007.02.018
[25]	俞松仁, 姚琦, 周丽, 等. 基于文献分析的肥胖痰湿证方药规律研究[J]. 光明中医,2020,35(6):795−800. [Yu S R, Yao Q, Zhou L, et al. Research on the prescription rules of obesity with phlegm dampness syndrome based on literature analysis[J]. Guangming Journal of Chinese Medicine,2020,35(6):795−800. doi: 10.3969/j.issn.1003-8914.2020.06.001
[26]	张均伟, 侯粲, 杜昱光, 等. 基于高分辨质谱结合主成分分析技术评价发酵过程对六堡茶关键品质成分的影响[J]. 食品科技,2019,44(12):328−334. [Zhang J W, Hou C, Du Y G, et al. Effect of fermentation on chemical compositions of Liupao tea using high-resolution mass spectrometry combined with principal component analysis[J]. Food Science and Technology,2019,44(12):328−334.
[27]	Wu Y, Sun H L, Yi R K, et al. Anti-obesity effect of Liupao tea extract by modulating lipid metabolism and oxidative stress in high-fat-diet-induced obese mice[J]. Journal of Food Science,2021,86(1):215−227. doi: 10.1111/1750-3841.15551
[28]	Zhu K, Peng P, Wu N, et al. Preventive effect of Liupao tea polyphenols on HCl/ethanol-induced gastric injury in mice[J]. Journal of Food Quality,2020,2020:1−10.
[29]	Ding Q Z, Zhang B W, Zheng W, et al. Liupao tea extract alleviates diabetes mellitus and modulates gut microbiota in rats induced by streptozotocin and high-fat, high-sugar diet[J]. Biomedicine & Pharmacotherapy,2019,118:1−7.
[30]	Mao Y, Wei B Y, Teng J W, et al. Polysaccharides from Chinese Liupao dark tea and their protective effect against hyperlipidemia[J]. International Journal of Food Science & Technology,2018,53(3):599−607.
[31]	Zhao X H, Geng Q. Acid production and conversion of konjac glucomannan during in vitro colonic fermentation affected by exogenous microorganisms and tea polyphenols[J]. International Journal of Food Sciences and Nutrition,2016,67(3):274−282. doi: 10.3109/09637486.2016.1150437
[32]	中华中医药学会. 中医体质分类与判定(ZYYXH/T157-2009)[J]. 世界中西医结合杂志,2009,4(4):303−304. [China Association of Chinese Medicine. Classification and judgment of constitution of traditional Chinese medicine(ZYYXH/T157-2009)[J]. World Journal of Integrated Traditional and Western Medicine,2009,4(4):303−304.
[33]	Robert C E, Brian J H, Jose C C, et al. UCHIME improves sensitivity and speed of chimera detection[J]. Bioinformatics,2011,27(16):2194−2200. doi: 10.1093/bioinformatics/btr381
[34]	肖岚岚, 许健. 肝功能与血清学指标水平检验对脂肪肝诊断的作用研究[J]. 中国现代药物应用,2020,14(11):39−41. [Xiao L L, Xu J. Effect of liver function and serological index on the diagnosis of fatty liver[J]. Chinese Journal of Modern Drug Application,2020,14(11):39−41.
[35]	庄耀东, 高碧珍, 王永发, 等. 代谢综合征“痰证”的病位、病性兼杂特征及与胰岛素抵抗、生化指标的相关性研究[J]. 中华中医药杂志,2017,32(2):546−548. [Zhuang Y D, Gao B Z, Wang Y F, et al. Study on correlations between disease location, concurrent nature of disease, insulin resistance and biochemical indicator in metabolic syndrome with phlegm syndrome[J]. Chinese Journal of Traditional Chinese Medicine,2017,32(2):546−548.
[36]	白雪松, 刘鹤, 宋岩, 等. 基于16S rRNA技术分析当归超微粉对小鼠肠道菌群多样性的影响[J]. 现代食品科技,2021,37(43):25−32. [Bai X S, Liu H, Song Y, et al. Analysis of the effects of Angelica sinensis ultrafine powder on the diversity of intestinal flora in mice based on 16S rRNA technology[J]. Modern Food Science and Technology,2021,37(43):25−32.
[37]	Francesca D F, Edoardo P, Danilo E. Newly explored Faecalibacterium drivers is connected to age, lifestyle, geography and disease[J]. Current Biology,2020,30(24):4932−4943. doi: 10.1016/j.cub.2020.09.063
[38]	Kivenson V, Giovannoni S J. An expanded genetic code enables trimethylamine metabolism in human gut bacteria[J]. mSystems,2020,5(5):413−420.

[1]	cover[J]. Science and Technology of Food Industry, 2022, 43(21).
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[4]	cover[J]. Science and Technology of Food Industry, 2022, 43(10).
[5]	cover[J]. Science and Technology of Food Industry, 2022, 43(6).
[6]	Cover[J]. Science and Technology of Food Industry, 2022, 43(5).

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