ZHOU Feng, YAN Yangli, HUANG Kai, et al. Evaluation of in Vitro Anti-inflammatory and Antioxidant Activities and Analysis of Chemical Components in Different Extraction Parts of Lonicerae Flos[J]. Science and Technology of Food Industry, 2021, 42(8): 81−87. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020080208.
Citation: ZHOU Feng, YAN Yangli, HUANG Kai, et al. Evaluation of in Vitro Anti-inflammatory and Antioxidant Activities and Analysis of Chemical Components in Different Extraction Parts of Lonicerae Flos[J]. Science and Technology of Food Industry, 2021, 42(8): 81−87. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020080208.

Evaluation of in Vitro Anti-inflammatory and Antioxidant Activities and Analysis of Chemical Components in Different Extraction Parts of Lonicerae Flos

More Information
  • Received Date: August 20, 2020
  • Available Online: January 31, 2021
  • The aim of this study is to elucidate the material basis of Lonicerae Flos biological activity and provide a theoretical basis for its development and utilization, lipopolysaccharide (LPS)-induced cell inflammation model and H2O2-induced oxidative stress model were used to evaluate the anti-inflammatory and antioxidant activities of different extraction parts of Lonicerae Flos. The chemical constituents of the extractions with significant activities were analyzed by ultra-high pressure liquid chromatography tandem quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS). The results showed that the methanol extracts of Lonicerae Flos(50 μg/mL) have different levels of anti-oxidation and anti-inflammatory effects. The extraction site of saturated n-butanol effectively inhibited the inflammatory factors (NO、TNF-α and IL-6) of RAW264.7 cells, suggesting it has the strongest anti-inflammatory activity. The inhibition rates of inflammatory factors of NO, TNF-α and IL-6 were 66.40%, 13.14% and 79.66% respectively. 16 compounds in the water-saturated n-butanol extractive site were identified, and which mainly consisted of organic acids.The ethyl acetate extractive site has the strongest antioxidant ability. The antioxidant enzyme activity in RIN cells had increased about 2.51(SOD), 4.40(GSH), 8.89(CAT) times respectively. After preliminary identification, 12 compounds, including organic acids and saponins in the ethyl acetate site, were the main compound. Therefore, organic acids and saponins were the main chemical components of Lonicerae Flos, which had the antioxidant and anti-inflammatory activities.This article provides data support for further elucidating the material basis and development and utilization of Lonicerae Flos.
  • [1]
    国家药典委员会, 中华人民共和国药典(一部)[S]. 北京: 中国医药科技出版社, 2020: 32.
    [2]
    王志萍, 邓家刚, 王勤, 等. 山银花研究的最新进展[J]. 广西中医学院学报,2008,11(4):59−61.
    [3]
    李冲, 文翔昊, 郭露, 等. 山银花黄酮粗提物对急性心肌缺血氧化损伤大鼠的保护作用[J]. 中国动脉硬化杂志,2018,26(8):779−783, 835. doi: 10.3969/j.issn.1007-3949.2018.08.005
    [4]
    Han M H, Lee W S, Nagappan A, et al. Flavonoids isolated from flowers of Lonicera japonica Thunb. Inhibit inflammatory responses in BV2 microglial cells by suppressing TNF-alpha and IL-beta through PI3K/Akt/NF-kb signaling pathways[J]. Phytotherapy Research: PTR,2016,30(11):1824−1832. doi: 10.1002/ptr.5688
    [5]
    Hu X, Chen L, Shi S, et al. Antioxidant capacity and phenolic compounds of Lonicerae macranthoides by HPLC-DAD-QTOF-MS/MS[J]. Journal of Pharmaceutical and Biomedical Analysis,2016,124:254−260. doi: 10.1016/j.jpba.2016.03.008
    [6]
    杨兰, 刘东波, 舒利, 等. 不同基源“金银花”的功能和应用研究进展[J]. 食品与机械,2020,36(1):10−19.
    [7]
    Hou Y, Jiang J G. Origin and concept of medicine fo od homology and its application in modern functional foods[J]. Food & Function,2013,4(12):1727−1741.
    [8]
    李泮霖, 李楚源, 刘孟华, 等. 基于UFLC-Triple-Q-TOF-MS/MS技术的金银花、山银花化学成分比较[J]. 中南药学,2016,14(4):363−369. doi: 10.7539/j.issn.1672-2981.2016.04.007
    [9]
    Deniz B, Zulfiye G, Julie A M, et al. Pharmacologic overview of chlorogenic acid and its metabolites in chronic pain and inflammation[J]. Current Neuropharmacology,2020,18(3):216−228. doi: 10.2174/1570159X17666191021111809
    [10]
    Naveedv M, Hejazi V, Abbas M, et al. Chlorogenic acid (CGA): A pharmacological review and call for further research[J]. Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie,2018,97:67−74.
    [11]
    肖作为, 谢梦洲, 甘龙, 等. 山银花、金银花中绿原酸和总黄酮含量及抗氧化活性测定[J]. 中草药,2019,50(1):210−216. doi: 10.7501/j.issn.0253-2670.2019.01.031
    [12]
    陈晓白, 蒋夏荣, 杨秋元, 等. 山银花多糖提取工艺优化及其抗氧化活性研究[J]. 中国食品添加剂,2018,11:155−161. doi: 10.3969/j.issn.1006-2513.2018.11.019
    [13]
    李泮霖, 贺利利, 李楚源, 等. 金银花和山银花抗急性口腔炎症作用比较[J]. 中山大学学报(自然科学版),2016,55(4):118−122.
    [14]
    邹璐. 山银花抗炎抗菌谱效关系研究[D]. 贵阳: 贵州大学, 2019.
    [15]
    张贤, 郭泽, 张冲, 等. 黄芪总黄酮对脂多糖体外诱导的RAW264.7细胞的细胞因子和NO分泌水平的影响[J]. 中国兽医科学,2015,45(3):321−324.
    [16]
    于笛, 周伟, 郭增旺, 等. 绿豆寡肽对脂多糖诱导巨噬细胞RAW264.7的抗炎作用[J]. 中国食品学报,2020,20(8):41−48.
    [17]
    裴晋红, 宋英达, 武翠玲. 硒化牡丹籽粕多糖的抗氧化研究[J]. 食品与发酵工业,2020,46(17):174−179.
    [18]
    Oi L W, Chen C Y, Li P. Structural characterization and identification of iridoid glycosides, saponins, phenolic acids and flavonoids in flos Lonicerae japonicae by a fast liquid chromatography method with diode-array detection and time-of-flight mass spectrometry[J]. Rapid Communications in Mass Spectrometry: RCM,2009,23(19):3227−3242. doi: 10.1002/rcm.4245
    [19]
    朱姮, 崔莉, 刘倩, 等. HPLC-DAD-ESI-Q-TOF/MS法测定金银忍冬花中的化学成分[J]. 中草药,2017,48(11):2300−2305.
    [20]
    Song Y, LI S L, Wu M H, et al. Qualitative and quantitative analysis of iridoid glycosides in the flower buds of Lonicera species by capillary high performance liquid chromatography coupled with mass spectrometric detector[J]. Analytica Chimica Acta,2006,564(2):211−218. doi: 10.1016/j.aca.2006.01.068
    [21]
    Iwahashi H, Negoro Y, Ikeda A, et al. Inhibition by chlorogenic acid of haematin-catalysed retinoic acid 5, 6-epoxidation[J]. Biochem J,1986,239(3):641−646. doi: 10.1042/bj2390641
    [22]
    Seo O N, Kim G-S, Park S, et al. Determination of polyphenol components of Lonicera japonica Thunb. using liquid chromatography–tandem mass spectrometry: Contribution to the overall antioxidant activity[J]. Food Chemistry,2012,134(1):572−577. doi: 10.1016/j.foodchem.2012.02.124
    [23]
    Lee E J, Kim J S, Kim H P, et al. Phenolic constituents from the flower buds of Lonicera japonica and their 5-lipoxygenase inhibitory activities[J]. Food Chemistry,2009,120(1):134−139.
    [24]
    Ren M T, Chen J, Song Y, et al. Identification and quantification of 32 bioactive compounds in Lonicera species by high performance liquid chromatography coupled with time-of-flight mass spectrometry[J]. Journal of Pharmaceutical and Biomedical Analysis,2008,48(5):1351−1360. doi: 10.1016/j.jpba.2008.09.037
    [25]
    Liu J, Zhang J, Wang F, et al. Isolation and characterization of new minor triterpenoid saponins from the buds of Lonicera macranthoides[J]. Carbohydrate Research,2013,370:76−81. doi: 10.1016/j.carres.2013.01.019
    [26]
    Kakuda R, Imai M, Yaoita Y, et al. Secoiridoid glycosides from the flower buds of Lonicera japonica[J]. Phytochemistry,2000,55(8):879−881. doi: 10.1016/S0031-9422(00)00279-X
    [27]
    Chen Y, Feng X, Jia X, et al. Triterpene glycosides from Lonicera. Isolation and structural determination of seven glycosides from flower buds of Lonicera macranthoides[J]. Chemistry of Natural Compounds,2008,44(1):39−43. doi: 10.1007/s10600-008-0011-2
    [28]
    Chen C X, Wang W W, Wei N I, et al. Triterpenoid glycosides from the Lonicera japonica[J]. Acta Botanica Yunnanica,2000,22(2):201−208.
    [29]
    Lee S J, Shin E J, Son K H, et al. Anti-inflammatory activity of the major constituents of Lonicera japonica[J]. Archives of Pharmacal Research,1995,18(2):133−135. doi: 10.1007/BF02979147
    [30]
    张伟敏, 魏静, 胡振, 等. 灰毡毛忍冬提取纯化物抗氧化性研究[J]. 食品科学,2008(3):109−112. doi: 10.3321/j.issn:1002-6630.2008.03.016
    [31]
    Song W X, Yang Y C, Shi J G. Two new β -hydroxy amino acid-coupled secoiridoids from the flower buds of Lonicera japonica: Isolation, structure elucidation, semisynthesis, and biological activities[J]. Chinese Chemical Letters,2014,25(9):1215−1219. doi: 10.1016/j.cclet.2014.05.037
    [32]
    Zhou Y F, Li L, Sun L, et al. In comparison with vitamin C and butylated hydroxytoluene, the antioxidant capacity of aqueous extracts from buds and flowers of Lonicera japonica Thunb[J]. Journal of Traditional Chinese Medicine,2018,38(3):373−379. doi: 10.1016/S0254-6272(18)30627-7
    [33]
    张明. 几种体外抗氧化检测方法的评价研究[D]. 西安: 陕西师范大学, 2010.
    [34]
    曾安琪, 华桦, 陈朝荣, 等. 金银花、山银花抗炎药理作用研究[J]. 中国中药杂志,2020,45(16):3938−3944.
  • Cited by

    Periodical cited type(18)

    1. 周水清,周政. 在饲粮中添加艾草粉对散养鸡的生产性能和抗病能力的影响. 青海畜牧兽医杂志. 2025(01): 13-15+35 .
    2. 雷娇,邵起菊,肖欣,李志荣,洪金伶,王森,陈荣祥. 基于HPLC-ECD测定鱼腥草叶多酚含量及抗氧化活性. 食品工业科技. 2024(04): 221-228 . 本站查看
    3. 孙立秋,王丹,赵英楠,时志春,李军,王金兰,赵明,张树军. 一测多评法测定艾叶中7个黄酮类成分的含量. 药物分析杂志. 2024(05): 806-815 .
    4. 郑文涛,刘元会,魏光强,赵兴文,黄艾祥. 蜂蜜桑茶复合饮料的工艺优化及其抗氧化活性研究. 食品科技. 2024(04): 100-107 .
    5. 崔英才,蒋梦宇,刘梦茹,来金良,姚型文,王正国,燕磊,刘永学. 艾草粉对蛋鸡生产性能、蛋品质、抗氧化功能及粪便评分的影响. 饲料研究. 2024(12): 39-43 .
    6. 纪德佳,苏慧,刘少雄,廖小兰,侯春久. 黎川县艾叶不同采收期总黄酮及山奈酚、异泽兰黄素的测定. 山东化工. 2024(24): 118-120+124 .
    7. 王文哲,梁芳,类成通,孔祥青,王裕玉. 艾蒿及其提取物在动物生产中的应用研究进展. 饲料研究. 2024(24): 158-162 .
    8. 廖富友,姚炳浓,杨娇一,王姣,祝保国,杨胜林. 饲粮添加艾叶粉对青年期蛋鸭生长性能、抗氧化功能及免疫性能的影响. 中国家禽. 2023(01): 76-81 .
    9. 李胜有,荣冬芸,潘卫东,郑志昌. 艾叶的活性成分、功效及其在动物饲养中的应用研究进展. 饲料研究. 2023(01): 137-141 .
    10. 程银水,张勇,秦志旺,董阳,李志浩,郝新才. 超高效液相色谱指纹图谱结合化学模式识别分析艾叶质量. 理化检验-化学分册. 2023(07): 837-843 .
    11. 孙君燕,孙敏,宋诗清,王化田,冯涛,姚凌云,俞文华. 香榧纯露的挥发性成分及抗氧化活性研究. 食品工业科技. 2022(03): 40-47 . 本站查看
    12. 周永强,赵春丽,殷鑫,周涛,韩伟,张永萍. 小花清风藤醇提物的化学成分及体外抗氧化活性研究. 中国药房. 2022(05): 530-534 .
    13. 陈誉华,马钱波,魏元浩,黄倩倩. 艾叶不同极性溶剂提取物的抗氧化能力的研究. 饲料研究. 2022(14): 64-68 .
    14. 赵永恒,张勇,秦志旺,董阳,汤哲伟,陈富超,罗雪,郝新才. 气相色谱-质谱指纹图谱结合化学计量学方法分析不同产地艾叶挥发油的差异. 理化检验-化学分册. 2022(11): 1277-1282 .
    15. 朱平平,覃智恒,彭慧倩,刘育文,刘静,陈锦涛,彭颖. 艾叶中多糖和黄酮联合提取工艺及抗氧化性研究. 广州化工. 2022(23): 66-69+98 .
    16. 王美英,李化强,吴菲菲. 超声波辅助提取竹叶鸡爪茶总黄酮的工艺优化及抗氧化、抑菌活性研究. 粮食与油脂. 2021(06): 105-111 .
    17. 王举翠,李明芝. 鲜芦根荷叶复合饮料研制及对运动耐力的影响研究. 食品安全质量检测学报. 2021(17): 7021-7029 .
    18. 梅瑜,徐世强,顾艳,孙铭阳,周芳,李静宇,张闻婷,王继华. 红脚艾蒿的转录组解析. 广东农业科学. 2021(12): 174-180 .

    Other cited types(10)

Catalog

    Article Metrics

    Article views (502) PDF downloads (70) Cited by(28)

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return