LIU Mengpei, LI Jia, JIN Xueyuan, et al. Analysis of Aroma Components in Water Extract of Eucommia ulmoides Leaf Fermented by Different Lactobacillus Species [J]. Science and Technology of Food Industry, 2021, 42(9): 36−43. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020070173.
Citation: LIU Mengpei, LI Jia, JIN Xueyuan, et al. Analysis of Aroma Components in Water Extract of Eucommia ulmoides Leaf Fermented by Different Lactobacillus Species [J]. Science and Technology of Food Industry, 2021, 42(9): 36−43. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020070173.

Analysis of Aroma Components in Water Extract of Eucommia ulmoides Leaf Fermented by Different Lactobacillus Species

More Information
  • Received Date: July 15, 2020
  • Available Online: March 03, 2021
  • In order to research the effect of different Lactobacillus species on the aroma components of water extract of Eucommia ulmoides leaf, electronic nose (E-nose) and simultaneous distillation extraction (SDE) combined with gas chromatography-mass spectrometry (GC-MS) was used to analyze the volatile flavor composition of water extract of Eucommia ulmoides leaf fermented by Lactobacillus plantarum, Lactobacillus bulgaricus, Lactobacillius acidophilus and Streptococcus thermophilus. The results of SDE-GC-MS showed that 31 aroma components were identified in unfermented samples, with aldehyde being the most abundant constituents (14.3591%). The volatile components identified by Lactobacillus plantarum, Lactobacillus bulgaricus, Lactobacillius acidophilus and Streptococcus thermophilus after fermentation were 45, 38, 51 and 43, with ketones (18.8255%), heterocycles (25.7828%), alcohols (18.3376%) and alcohols (14.1481%) as the highest relative contents, respectively. In principal component analysis (PCA) and Loading Analysis (LoA) the cumulative contribution of the first 2 principal components accounted for greater than 95%, suggesting that the E-nose sensors had good recognition performance and discrimination between samples on the basis of their flavor characteristics was excellent with clear distinctions.The relative odor activity value (ROAV) indicated that Lactobacillus fermentation increased the content of key aroma components in Eucommia ulmoides leaf extract, and reduced its green leaf flavor. After Lactobacillus fermentation, the extract of Eucommia ulmoides leaf not only had more volatile components, but also significantly increased the content of key aroma components, which was more conducive to the improvement of aroma in the extract of Eucommia ulmoides. The results of this study provide a reference for the later research and development of Eucommia ulmoides leaf.
  • [1]
    张子东, 付冬梅, 张威鹏, 等. HPLC法同时测定不同生长年限不同部位杜仲中5种苯丙素类成分[J]. 食品科学,2019,40(8):186−191. doi: 10.7506/spkx1002-6630-20180417-231
    [2]
    张丽华, 李珍珠, 乜晓爽, 等. 植物乳杆菌发酵杜仲鲜叶饮料的研制[J]. 食品科技,2019,44(2):129−133.
    [3]
    Wang J Y, Yuan Y, Chen X J, et al. Extract from Eucommia ulmoides Oliv. ameliorates arthritis via regulation of inflammation, synoviocyte proliferation and osteoclastogenesis in vitro and in vivo[J]. Journal of Ethnopharmacology,2016,194(194):609−616.
    [4]
    Shao P, Zhang J F, Chen X X, et al. Microwave-assisted extraction and purification of chlorogenic acid from by-products ofEucommia ulmoides Oliver and its potential anti-tumor activity[J]. Journal of Food Science and Technology,2015,52(8):4925−4934. doi: 10.1007/s13197-014-1571-8
    [5]
    Xing Y F, He D, Wang Y, et al. Chemical constituents, biological functions and pharmacological effects for comprehensive utilization of Eucommia ulmoides Oliver[J]. Food Science and Human Wellness,2019,8(2):177−188. doi: 10.1016/j.fshw.2019.03.013
    [6]
    朱周靓, 严峻, 郑云燕, 等. 杜仲叶安全性的毒理学评价[J]. 预防医学,2017,29(5):443−448.
    [7]
    Takamura C, Hirata T, Yamaguchi Y, et al. Studies on the chemical constituents of green leaves of Eucommia ulmoides Oliv.[J]. Journal of Natural Medicines,2007,61(2):220−221. doi: 10.1007/s11418-006-0027-5
    [8]
    Takemoto M. Method of producing fermented tea drink rich in theaflavins: US20090934693[P]. 2009-3-27. https://www.surechembl.org/document/US-20110064851-A1.
    [9]
    向敏, 徐茂, 王子涵, 等. 混合乳酸菌发酵对桑叶中挥发性关键异味组分的影响[J/OL]. 食品与发酵工业: 1−11[2020-08-22]. https://doi.org/10.13995/j.cnki.11-1802/ts.024204.
    [10]
    王翔, 胡凤杨, 杨秋玲, 等. 杜仲叶的营养评价及体外抗氧化活性分析[J]. 食品工业科技,2019,40(21):290−299.
    [11]
    陆宽, 王雪雅, 孙小静, 等. 电子鼻结合顶空SPME-GC-MS联用技术分析贵州不同品种辣椒发酵后挥发性成分[J]. 食品科学,2018,39(4):199−205. doi: 10.7506/spkx1002-6630-201804030
    [12]
    孙洁雯, 杨克玉, 李燕敏, 等. SDE结合GC-MS分析王致和臭豆腐中的特征香气成分[J]. 食品科学,2015,36(16):127−131. doi: 10.7506/spkx1002-6630-201516023
    [13]
    林杰, 江汉美, 卢金清. HS-SPME-GC-MS法分析杜仲和杜仲叶中挥发性成分[J]. 安徽农业科学,2018,46(10):165−166, 199. doi: 10.3969/j.issn.0517-6611.2018.10.050
    [14]
    李岩, 赵德刚. 杜仲挥发性成分测定及差异性研究[J]. 中华中医药杂志,2010,25(10):1641−1644.
    [15]
    罗威, 耿敬章. 杜仲叶绿茶复合饮料的工艺优化[J]. 食品工业,2012,33(5):28−30.
    [16]
    中华人民共和国国家卫生和计划生育委员会. GB/T 4789.35-2016 《食品安全国家标准, 食品微生物学检验, 乳酸菌检验》[S]. 北京: 中国标准出版社, 2017.
    [17]
    中华人民共和国国家卫生和计划生育委员会. GB 4789.3-2016 《食品安全国家标准, 食品微生物学检验, 大肠菌群计数》[S]. 北京: 中国标准出版社, 2017.
    [18]
    姚远, 陶玉贵, 葛飞, 等. 两种酿酒酵母发酵对桑葚米酒理化品质及香气成分的影响[J]. 食品工业科技,2019,40(24):242−250, 261.
    [19]
    Liu M, Han X M, Tu K, et al. Application of electronic nose in Chinese spirits quality control and flavour assessment[J]. Food Control,2012,26(2):564−570 (in Chinese). doi: 10.1016/j.foodcont.2012.02.024
    [20]
    Dong W J, Hu R S, Long Y Z, et al. Comparative evaluation of the volatile profiles and taste properties of roasted coffee beans as affected by drying method and detected by electronic nose, electronic tongue, and HS-SPME-GC-MS[J]. Food Chemistry,2019,272(272):723−731.
    [21]
    Yang W J, Yu J, Pei F, et al. Effect of hot air drying on volatile compounds of Flammulina velutipes detected by HS-SPME-GC-MS and electronic nose[J]. Food Chemistry,2016,196(196):860−866.
    [22]
    蓬桂华, 李文馨, 殷勇, 等. 电子鼻和电子舌在分析桑果汁风味上的应用[J/OL]. 食品工业科技: 1−9[2020-03-14]. http://kns.cnki.net/kcms/detail/11.1759.TS.20191213.1655.040.html.
    [23]
    周围, 王波, 刘倩倩, 等. 基于电子鼻和GC-MS对不同品种玫瑰“活体”香气的研究[J]. 香料香精化妆品,2017,2 (2):1−6, 72. doi: 10.3969/j.issn.1000-4475.2017.02.001
    [24]
    范霞, 陈荣顺. SPME/GC-MS法结合电子鼻技术测定茶叶中的香气成分[J]. 检验检疫学刊,2019,29(2):1−6, 12.
    [25]
    刘威, 张永瑞, 鲁静, 等. 不同加工工艺刺槐花代用茶香气成分分析[J/OL]. 食品工业科技: 1−11[2020-08-17]. http://kns.cnki.net/kcms/detail/11.1759.TS.20200721.0859.002.html.
    [26]
    Wu Y S, Zhang W W, Yu W J, et al. Study on the volatile composition of table grapes of three aroma types[J]. LWT,2019,115(1):1−9.
    [27]
    Du X F, Chad E. F, Michael C Q. Volatile composition and odour-activity value of thornless ‘Black Diamond’ and ‘Marion’ blackberries[J]. Food Chemistry,2009,119(3):1127−1134.
    [28]
    杨婷, 祝霞, 赵宾宾, 等. 不同酿酒酵母对甜瓜酒香气品质的影响分析[J]. 食品科学,2015,36(14):145−150. doi: 10.7506/spkx1002-6630-201514028
    [29]
    Pato R N, Barreiro C G, Grande B C. Aroma potential of Brancellao grapes from different cluster positions[J]. Food Chemistry,2012,132(1):112−124. doi: 10.1016/j.foodchem.2011.10.042
    [30]
    李维妮, 郭春锋, 张宇翔, 等. 气相色谱-质谱法分析乳酸菌发酵苹果汁香气成分[J]. 食品科学,2017,38(4):146−154. doi: 10.7506/spkx1002-6630-201704024
    [31]
    石渝凤, 邸太妹, 杨绍兰, 等. 花香型红茶加工过程中香气成分变化分析[J]. 食品科学,2018,39(8):167−175. doi: 10.7506/spkx1002-6630-201808027
    [32]
    杨素华, 安家成, 谭桂菲, 等. 樟树挥发油成分分析[J]. 广西林业科学,2018,47(3):350−353. doi: 10.3969/j.issn.1006-1126.2018.03.021
    [33]
    王秋亚, 马艳阳. 肉桂精油的成分分析、抑菌和抗氧化活性及在食品保鲜中的应用进展[J]. 中国调味品,2020,45(3):183−187.
    [34]
    张华. 反式-2-烯醛类化合物和反, 反-2, 4-二烯醛类化合物在香精中的应用[J]. 香料香精化妆品,2006,2(2):30−34.
    [35]
    王华, 宋建强, 梁艳英, 等. 搅拌棒萃取-气相色谱-质谱联用法分析‘媚丽’桃红葡萄酒中的香气成分[J]. 食品科学,2014,35(2):177−181. doi: 10.7506/spkx1002-6630-201402033
    [36]
    唐健. 甲基紫罗兰酮的合成[J]. 河北化工,2011,34(2):56−58.
    [37]
    朱晓凤, 刘政权, 宛晓春, 等. SDE和HS-SPME结合GC-MS分析霍山黄大茶香气成分的比较[J]. 食品科学,2020,41(4):214−221. doi: 10.7506/spkx1002-6630-20190110-136
    [38]
    吕艳杰. 同时蒸馏萃取和固相微萃取对双孢蘑菇风味物质的提取分析[J]. 食品科学,2015,36(10):185−188. doi: 10.7506/spkx1002-6630-201510037
  • Cited by

    Periodical cited type(5)

    1. 赵斌,李娣娣,袁源,韦婷. 发酵型功能酒的现代研究进展. 酿酒. 2025(02): 43-49 .
    2. 陈乙源,洪嘉欣,黄河,王硕,武亚帅,赵东瑞,孙金沅,黄明泉,孙啸涛. 基于GC×GC-MS分析不同等级浓香型白酒的关键风味化合物. 中国酿造. 2025(03): 29-35 .
    3. 舒翔,邱奇琦. 果酒降酸专利技术分析. 安徽农学通报. 2024(04): 97-101 .
    4. 廖娟,李嘉宇,黄杰惠,陈博慧,杨涛. 耐低温酵母的分离鉴定及对低温酿造米酒品质的影响. 食品工业科技. 2024(07): 159-166 . 本站查看
    5. 王爱灵,张梅,申娜娜,徐梦文,章骏伟,李瑞龙,兰伟. 黑果腺肋花楸果酒生产工艺研究进展. 滁州学院学报. 2024(02): 21-28 .

    Other cited types(0)

Catalog

    Article Metrics

    Article views (233) PDF downloads (24) Cited by(5)

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return