PENG Yun, LI Guo, LIU Xueyan, et al. SPME/GC-MS Analysis of Aroma Quality of Black Tea from Different Producing Areas[J]. Science and Technology of Food Industry, 2021, 42(9): 228−235. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306. 2020060025.
Citation: PENG Yun, LI Guo, LIU Xueyan, et al. SPME/GC-MS Analysis of Aroma Quality of Black Tea from Different Producing Areas[J]. Science and Technology of Food Industry, 2021, 42(9): 228−235. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306. 2020060025.

SPME/GC-MS Analysis of Aroma Quality of Black Tea from Different Producing Areas

More Information
  • Received Date: June 02, 2020
  • Available Online: March 02, 2021
  • In order to explore the material basis of black tea aroma and the characteristics of main aromatic components in Yunnan, Fujian and Yichang, the volatile aroma components of black tea from three different regions were analyzed by using sensory evaluation method and headspace solid phase microextraction (HS-SPME) combined with gas chromatography-mass spectrometry (GC-MS).The results showed that all the 6 kinds of black teas were dominated by sweet aroma, but dianhong had strong sweet aroma, fuding black tea had both floral fragrance and sweet aroma, and Yichang black tea had pure sweet aroma.A total of 67 kinds of aroma compounds were isolated and identified in 6 kinds of black tea by GC-MS, including alcohols, aldehydes, ketones, esters, olefins, alkanes and acids.Among them, the main aroma contribution components were alcohol compounds.The content of aroma components in various black tea was higher.The main aroma components include: Linalool and its oxides; 3,7,11-Trimethyldodeca-1,6,10-trien-3-ol; 5,9-Undecadien-2-one, 6,10-dimethyl-, (E)-;3-Buten-2-one, 4-(2,6,6-trimethyl-2-cyclohexen-1-yl)-, (E)-; 5,9-Undecadien-2-one, 6,10-dimethyl-, (E)-;Methyl Salicylateetc.Among them, linalool and its oxide were characteristic aroma components in Yunnan black tea;β-dihydroionone and β-cylinne were characteristic aroma components in Fuding black tea, while in Yichang black tea, geranyl alcohol and palmitic acid were the characteristic aroma components.The study clarified the chemical basis of the formation by the aroma quality of black tea from different origins from the perspective of aroma components, which provided a theoretical basis for the quality improvement and origin identification of black tea.
  • [1]
    V-R Sinija, Mishra H-N. Fuzzy analysis of sensory data for quality evaluation and ranking of instant green tea powder and granules[J]. Food and Bioprocess Technology,2011,4(3):408−416. doi: 10.1007/s11947-008-0163-x
    [2]
    刘飞, 王云, 张厅, 等. 红茶加工过程香气变化研究进展[J]. 茶叶科学,2018,38(1):9−19. doi: 10.3969/j.issn.1000-369X.2018.01.002
    [3]
    潘科, 冯林, 陈娟, 等. HS-SPME-GC-MS联用法分析不同通氧发酵加工工艺红茶香气成分[J]. 食品科学,2015,36(8):181−186. doi: 10.7506/spkx1002-6630-201508033
    [4]
    Ziyan Wang, Han Binsong, Jing Wangfang, et al. Effects of different steeping temperatures on the leaching of aroma components in black tea by SPME-GC-MS coupled with chemometric method[J]. Journal of Aoac International,2019,102(6):1834−1844. doi: 10.1093/jaoac/102.6.1834
    [5]
    王梦琪, 朱荫, 张悦, 等. 茶叶挥发性成分中关键呈香成分研究进展[J]. 食品科学,2019,40(23):341−349. doi: 10.7506/spkx1002-6630-20181015-132
    [6]
    Sine Yener, Sanchez-Lopez Jose-A, Granitto Pablo-M, et al. Rapid and direct volatile compound profiling of black and green teas (Camellia sinensis) from different countries with PTR-ToF-MS[J]. Talanta,2016:15245−53.
    [7]
    杨娟, 李中林, 袁林颖, 等. 重庆不同茶树品种加工工夫红茶香气成分分析[J]. 西南大学学报(自然科学版),2019,41(6):21−27.
    [8]
    张娅楠, 欧伊伶, 覃丽, 等. 红茶中香气物质的形成及工艺对其影响的研究进展[J]. 食品工业科技,2019,40(11):351−357.
    [9]
    刘聪, 张文杰, 严亮, 等. 顶空固相微萃取结合GC/MS分析玫瑰香型滇红茶香气成分[J]. 食品科技,2017,42(8):300−305.
    [10]
    宛晓春. 茶叶生物化学[M]. 北京: 中国农业出版社, 2003.
    [11]
    徐元骏, 何靓, 贾玲燕, 等. 不同地区及特殊品种红茶香气的差异性[J]. 浙江大学学报(农业与生命科学版),2015,41(3):323−330.
    [12]
    Xiaohua Chen, Chen Dejing, Jiang Hai, et al. Aroma characterization of Hanzhong black tea (Camellia sinensis) using solid phase extraction coupled with gas chromatography-massspectrometry and olfactometry and sensory analysis[J]. Food Chemistry,2019:274130−136.
    [13]
    王秋霜, 陈栋, 许勇泉, 等. 中国名优红茶香气成分的比较研究[J]. 中国食品学报,2013,13(1):195−200.
    [14]
    任洪涛, 周斌, 夏凯国, 等. 不同级别云南工夫红茶香气成分比较分析[J]. 南方农业学报,2012,43(4):489−492. doi: 10.3969/j:issn.2095-1191.2012.04.489
    [15]
    任洪涛, 周斌, 秦太峰, 等. 基于多元统计分析的云南红茶香气质量评价[J]. 现代食品科技,2013,29(12):3006−3013.
    [16]
    Chengying Ma, Li Junxing, Chen Wei, et al. Study of the aroma formation and transformation during the manufacturing process of oolong tea by solid-phase micro-extraction and gas chromatography-mass spectrometry combined with chemometrics[J]. Food Research International (Ottawa, Ont.),2018:108.
    [17]
    Zechang Liu, Wang Liping, Liu Yumei. Rapid differentiation of Chinese hop varieties (Humulus lupulus) using volatile fingerprinting by HS-SPME-GC-MS combined with multivariate statistical analysis[J]. Journal of the Science of Food and Agriculture,2018,98(10):3758−3766. doi: 10.1002/jsfa.8889
    [18]
    Patcharee Pripdeevech, Rothwell Joseph, D'Souza Priya-E, et al. Differentiation of volatile profiles of Thai Oolong tea No. 12 provenances by SPME-GC-MS combined with principal component analysis[J]. International Journal of Food Properties,2018:203S2450−S2462.
    [19]
    程超, 杜芬妮, 李伟, 等. 基于9种风味物质的利川红SPME优化及PLS-DA风味识别[J/OL]. 食品科学: 1-13[2021-02-19]. http://kns.cnki.net/kcms/detail/11.2206.TS.20200601.1400.044.html.
    [20]
    P-Okinda Owuor, Obanda Martin, Nyirenda Hastings-E, et al. Influence of region of production on clonal black tea chemical characteristics[J]. Food Chemistry,2008,108(1):263−271. doi: 10.1016/j.foodchem.2007.09.017
    [21]
    Kele-A-C Vespermann, Paulino Bruno-N, Barcelos Mayara-C-S, et al. Biotransformation of α- and β-pinene into flavor compounds[J]. Applied Microbiology and Biotechnology,2017,101(5):1805−1817. doi: 10.1007/s00253-016-8066-7
    [22]
    章港, 黄文静, 徐珩, 等. 基于气质联用技术的“祁门香”特征性成分分析[J]. 茶叶通讯,2020,47(1):96−101. doi: 10.3969/j.issn.1009-525X.2020.01.017
    [23]
    X-Q Zheng, Li Q-S, Xiang L-P, et al. Recent advances in volatiles of teas[J]. Molecules,2016,21(3):338. doi: 10.3390/molecules21030338
    [24]
    金友兰, 黄甜, 蒋容港, 等. 不同类型发花砖茶特征香气成分研究[J/OL]. 食品与发酵工业: 1−11[2021-02-19]. https://doi.org/10.13995/j.cnki.11-1802/ts.025075.
    [25]
    肖凌. 十种香型凤凰单丛茶香气成分分析[D]. 重庆: 西南大学, 2018.
    [26]
    Suyoung Kang, Yan Han, Zhu Yin, et al. Identification and quantification of key odorants in the world's four most famous black teas[J]. Food Research International,2019:12173−83.
    [27]
    S Lv, Wu Y, Li C, et al. Comparative analysis of Pu-erh and Fuzhuan teas by fully automatic headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry and chemometric methods[J]. J Agric Food Chem,2014,62(8):1810−1818. doi: 10.1021/jf405237u
    [28]
    马士成, 刘春梅, 王梦琪, 等. 六堡茶挥发性成分中的关键香气成分研究[J]. 食品科学,2020,41(20):191−197.
    [29]
    王维皓, 杨立新. 羌活挥发性成分GC-MS分析[J]. 中国实验方剂学杂志,2020,26(23):153−160.
    [30]
    M-Q Wang, Ma W-J, Shi J, et al. Characterization of the key aroma compounds in Longjing tea using stir bar sorptive extraction (SBSE) combined with gas chromatography-mass spectrometry (GC-MS), gas chromatography-olfactometry (GC-O), odor activity value (OAV), and aroma recombination[J]. Food Res Int,2020:130108908.
    [31]
    王秋霜, 乔小燕, 操君喜, 等. 广东单丛茶树品种红茶香气成分的GC-MS分析[J]. 食品科学,2015,36(4):114−118.
    [32]
    Robin Joshi, Gulati Ashu. Fractionation and identification of minor and aroma-active constituents in Kangra orthodox black tea[J]. Food Chemistry,2015:167290−298.
    [33]
    曹晓念, 周志磊, 刘青青, 等. 基于香气成分的红茶品种比较分析[J]. 食品与发酵科技,2020,56(3):118−122.
    [34]
    罗学平, 李丽霞. SPME-GC-MS联用分析川红、滇红和祁红香气成分[J]. 宜宾学院学报,2016,16(6):102−107. doi: 10.3969/j.issn.1671-5365.2016.06.025
    [35]
    廉明, 吕世懂, 吴远双, 等. 我国4种红茶的挥发性成分分析[J]. 热带亚热带植物学报,2015,23(3):301−309. doi: 10.11926/j.issn.1005-3395.2015.03.011
    [36]
    赵瑶, 宋晓东, 黄啟亮, 等. 宜昌红茶代表产品的香气成分研究[J]. 湖北农业科学,2016,55(24):6493−6497.
    [37]
    郑鹏程, 刘盼盼, 龚自明, 等. 湖北红茶特征性香气成分分析[J]. 茶叶科学,2017,37(5):465−475. doi: 10.3969/j.issn.1000-369X.2017.05.005
  • Cited by

    Periodical cited type(24)

    1. 骆春萍,蔡树芸,黄雅瑜,林米妮,郑海龙,杨光乐,张怡评. 岩藻多糖制备工艺及其在护肤品中的应用进展. 香料香精化妆品. 2025(02): 23-28+110 .
    2. 刘松,刘红全,李慧敏,徐程浩,王燕姿,藏颖. 拟微绿球藻胞内多糖提取条件优化及其生物活性研究. 食品科技. 2024(03): 233-242 .
    3. 刘欣雨,胡蔚然,李铮凯,吉玮,倪潇. 藻胶实验室:澎湃荧光海. 大学化学. 2024(05): 396-404 .
    4. 沈康,郭瑞成,徐天旭,王伟华. DEAE-52纤维素柱层析纯化处理对西梅可溶性膳食纤维的影响. 食品与发酵工业. 2024(17): 209-217 .
    5. 张田,黄雨洋,刘琳琳,吕铭守,朱颖,孙冰玉,朱秀清. 多糖对高水分挤压植物蛋白肉结构及品质影响的研究进展. 食品科学. 2024(22): 341-350 .
    6. Changhui YAN,Mingxuan PAN,Lihua GENG,Quanbin ZHANG,Yadong HU,Jing WANG,Sujuan YE. A novel enzyme-assisted one-pot method for the extraction of fucoidan and alginate oligosaccharides from Lessonia trabeculata and their bioactivities. Journal of Oceanology and Limnology. 2024(06): 1998-2012 .
    7. 唐天城,朱本伟,姚忠,孙芸,熊强. 石莼多糖及其寡糖结构、制备及活性的研究进展. 现代食品科技. 2023(05): 340-353 .
    8. 唐翠娥,周培源,刘延照,李艳. 基于原子力显微镜研究黄原胶分子结构的云母片处理方法优化. 食品工业科技. 2023(14): 60-66 . 本站查看
    9. 邱智超,勾宇春,张志鹏,王宗抗,孟品品,周进,姜玥璐. 藻类资源在农业种植业中的应用研究进展. 农业资源与环境学报. 2023(04): 840-851 .
    10. 张琨霖,王贺琪,郭庆彬,刘欢欢,梁宏合,王乐,李贞景. 桑黄子实体多糖的提取工艺优化、结构解析及免疫活性分析. 食品工业科技. 2023(20): 93-100 . 本站查看
    11. 黄卫红,董乐. 紫球藻胞外多糖酶解物的纯化、红外鉴定及其抗氧化活性. 泉州师范学院学报. 2023(05): 19-27 .
    12. 曾凡珂,潘蕾蔓,张祎,赖富饶,吴晖,张猛猛. 荸荠皮多糖的理化性质及抗氧化活性. 现代食品科技. 2022(03): 82-88+81 .
    13. 王朝群,杨燕,唐超,何希瑞. 天麻多糖提取分离方法和药理作用研究进展. 中国药事. 2022(04): 417-428 .
    14. 杨斯惠,向月,曹亚楠,任远航,彭镰心,时小东. 植物多糖的益生作用及其影响因素研究进展. 食品科学. 2022(11): 301-310 .
    15. 刘亚楠,李欢,蒋凡,傅玲琳,王彦波. 基于活性包装视角下的水产品保鲜机制研究进展. 食品科学. 2022(13): 285-291 .
    16. 陈超,谭书明,王画,杨笙,代晓桐. 刺梨及其活性成分对2型糖尿病小鼠糖脂代谢的影响. 食品科学. 2022(13): 146-154 .
    17. 郭建行,贾颂华,李博润,李珊珊,冀晓龙,刘延奇. 红海藻多糖提取、分离纯化及生物活性研究进展. 食品研究与开发. 2022(16): 216-224 .
    18. 孙艳宾,李宁,梁君玲,张慧婧,景大为,张川. 海藻酸钠提取工艺研究进展. 食品科技. 2022(08): 201-206 .
    19. 贾薇,余冬生,余养朝,冯占,胡明,张劲松,汪雯翰. 三相分离法制备金针菇提取物中多糖的理化性质及体外活性. 食用菌学报. 2022(05): 73-80 .
    20. 孔丽,朱月霞,周冰雪,刘志,薛旺迁,石锦峰,郑家勤,李姣姣,吉敬,秦昆明,董自波,沈金阳. 海蒿子多糖提取、纯化及其药理活性研究进展. 大连海洋大学学报. 2022(05): 894-902 .
    21. 高玥,许倩楠,蔡明刚,胡贤陈,田文静,陈海峰. 海洋来源药食同源品开发利用研究进展. 中草药. 2021(17): 5455-5464 .
    22. 陆伊俏,池海波,厉桂宁,陈纤,邱家港,余辉,方旭波,陈小娥. 可口革囊星虫体腔液多糖碱提工艺优化及其抗氧化性研究. 食品工业科技. 2021(19): 204-210 . 本站查看
    23. 刘小杰,倪辉. 海藻多糖在乳品工业中的应用研究进展. 乳业科学与技术. 2021(06): 31-38 .
    24. 林晓娟,苏志琛,陈继承. 海带多糖的结构特征、生物活性及其应用. 现代食品. 2021(24): 49-52 .

    Other cited types(16)

Catalog

    Article Metrics

    Article views (421) PDF downloads (76) Cited by(40)

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return