Optimization of Extraction Process and Biological Activities of Flavonoid Aglycones from <i>Ribes meyeri</i> Stem

LU Honglin; ZHAO Yayun; WANG Qiang; SHEN Haitao; XU Wenbin; HE Dajun

doi:10.13386/j.issn1002-0306.2020040162

Volume 42 Issue 3

Jan. 2021

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Science and Technology of Food Industry > 2021 > 42(3): 140-146. > DOI: 10.13386/j.issn1002-0306.2020040162

LU Honglin, ZHAO Yayun, WANG Qiang, SHEN Haitao, XU Wenbin, HE Dajun. Optimization of Extraction Process and Biological Activities of Flavonoid Aglycones from Ribes meyeri Stem[J]. Science and Technology of Food Industry, 2021, 42(3): 140-146. DOI: 10.13386/j.issn1002-0306.2020040162

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Optimization of Extraction Process and Biological Activities of Flavonoid Aglycones from Ribes meyeri Stem

College of Life Science, Analysis and Testing Center, Shihezi University, Shihezi 832000, China

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Received Date: April 14, 2020
Available Online: February 02, 2021

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Abstract

In order to optimize acid hydrolysis extraction process of 5 flavonoid aglycones(luteolin,quercetin,kaempferol,isorhamnetin and apigenin)from Ribes meyeri stem and evaluate their antioxidant activity and α-glucosidase inhibitory activity in vitro,the content of 5 flavonoid aglycones were determined simultaneously by UPLC-MS/MS. The acid hydrolysis extraction process of flavonoid aglycones of Ribes meyeri stem was optimized by single factor experiments and orthogonal test. The antioxidant and hypoglycemic activities of acid hydrolysis extract and 5 flavonoid aglycones from Ribes meyeri stem were evaluated by DPPH,ABTS free radical scavenging and α-glucosidase inhibitory activity assays. The results showed that the limit of detection and limit of quantification about the five flavonoid aglycones were 0.8~1.6 μg/L and 2.9~5.4 μg/L. The five flavonoid aglycones had good linear relationship(R²≥0.9962)within 3.2~207.0 μg/L. The precision,repeatability and stability of the method were good for the 5 flavonoids(RSD≤4.4%). The optimal extraction process of flavonoid aglycones from Ribes meyeri stem was established as follows:90% methanol solution(including 5% hydrochloric acid),the extraction temperature was 70 ℃,the extraction time was 100 min. In this optimized extraction conditions,the extraction rate of total flavonoid aglycones reached 4.06 mg/g(RSD<5%),which indicated the extraction method was stable and feasible. The acid-hydrolysis extract of Ribes meyeri stem and the 5 flavonoid aglycones showed significant α-glucosidase inhibitory activity and antioxidant activity. The α-glucosidase inhibitory activities of methanol acid-hydrolysis extract and 5 flavonoid aglycones were higher than the positive control,acarbose(IC₅₀:(53.84±2.41) mg/L). Quercetin had the best DPPH free radical scavenging activity(IC₅₀:(2.94±0.18) mg/L),while luteolin and kaempferol had higher ABTS free radical scavenging activity(IC₅₀:(5.34±0.10),(5.55±0.17) mg/L,respectively),and their antioxidant activities were higher than the positive control V_C. The established UPLC-MS/MS method is sensitive,reliable and accurate,which can be used for the simultaneous quantitative analysis of 5 flavonoid aglycones. The optimal acid hydrolysis process can effectively improve extraction rate of flavonoid aglycones from Ribes meyeri stem.

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[1]	新疆植物志编辑委员会编. 新疆植物志(第2卷,第2分册)[M]. 新疆:科技卫生出版社,1993:281-284.
[2]	杨永昌,何廷农,卢生莲,等. 藏药志[M]. 西宁:青海人民出版社,1991:404-405.
[3]	田贺,张志东,李亚东,等. 茶藨属植物果实营养成分分析[J]. 吉林农业大学学报,2009,31(5):621-623.
[4]	吴文杰,赵中辉,张元,等.药食同源植物LC-MS技术分析的研究进展[J]. 食品工业科技,2016,37(19):366-371.
[5]	Lucci P,Saurina J,Núñez O. Trends in LC-MS and LC-HRMS analysis and characterization of polyphenols in food[J]. TrAC Trends in Analytical Chemistry,2017,88:1-24.
[6]	Ganzera M,Sturm S. Recent advances on HPLC/MS in medicinal plant analysis-An update covering 2011-2016[J]. J Pharm Biomed Anal,2018,147:211-233.
[7]	李转红,郭寒,徐文斌,等. 新疆3种植物不同提取部位的抗氧化活性及其对PTP1B的抑制作用[J]. 中国实验方剂学杂志,2015,21(19):28-33.
[8]	Imran M,Salehi B,Aslam G T,et al. Kaempferol:Akey emphasis to its anticancer potential[J]. Molecules,2019,24(12):2277-2293.
[9]	Parhiz H,Roohbakhsh A,Soltani F,et al. Antioxidant and anti-inflammatory properties of the Citrus flavonoids hesperidin and hesperetin:An updated review of their molecular mechanisms and experimental models[J]. Phytotherapy Research,2015,29(3):323-331.
[10]	Gowd V,Jia Z,Chen W. Anthocyanins as promising molecules and dietary bioactive components against diabetes-A review of recent advances[J]. Trends in Food Science & Technology,2017,68:1-13.
[11]	Yang X L,Ma Y L,Li L R. β-Glucosidase from tartary buckwheat immobilization on bifunctionalized nano-magnetic iron oxide and its application in tea soup for aroma and flavonoid aglycone enhancement[J]. Food & Function,2019,10:5461-5472.
[12]	余沙,何珺,钱一鑫,等. 银杏叶提取物中银杏黄酮苷元的提纯工艺研究[J]. 华西药学杂志,2011,26(6):577-579.
[13]	Filipe P,Silva J N,Haigle J,et al. Contrasting action of flavonoidson phototoxic effects induced in human skin fibroblasts by UVA alone or UVA plus cyamemazine,a phototo xic neuroleptic[J].Photochem Photobiol Sci,2005,4(5):420-428.
[14]	Xu J,Cao J,Yue J,et al. New triterpenoids from acorns of Quercus liaotungensis,and their inhibitory activity against α-glucosidase,α-amylase and protein-tyrosine phosphatase1B[J]. Journal of Functional Foods,2018,41:232-239.
[15]	Thilina L G,Kalpa W S,Dinithi C P,et al. In-vitro antioxidant,hypoglycemic activityand identification of bioactive compounds in phenol-rich extract from the marine red algae Gracilaria edulis(Gmelin)silva[J]. Molecules,2019,24:3708-3725.
[16]	郭寒,葛娟,李鑫,等. 正交试验优化黑果悬钩子茎、叶总黄酮的提取纯化及其抗氧化活性[J]. 食品科学,2015,36(14):10-16.
[17]	李古月,刘佳鑫,才谦. 齿叶白鹃梅叶总黄酮提取物酸水解工艺及水解后苷元提取率测定[J]. 天津中医药,2014,31(5):306-309.
[18]	崔福顺,史得君,李官浩,等. 盐酸水解法制备人参茎叶黄酮苷元的工艺研究[J]. 食品科技,2015,40(8):208-211.
[19]	刘飞,李佳,张永倩. 栝楼雄株茎叶黄酮类化合物的分离及其清除DPPH能力研究[J]. 中草药,2016,47(23):4141-4145.
[20]	袁博,曹健,秦朗,等. 四种酚类化合物体外抗氧化活性的比较研究[J]. 食品工业,2018,39(9):200-204.
[21]	肖颖,余英才,左爱仁. 异鼠李素和槲皮素抗氧化活性研究[J]. 时珍国医国药,2012,23(5):1118-1120.
[22]	Guo N,Zhu Y,Jiang Y W,et al. Improvement of flavonoid aglycone and biological activity of mulberry leaves by solid-state fermentation[J]. Industrial Crops & Products,2020,148:112287.

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Optimization of Extraction Process and Biological Activities of Flavonoid Aglycones from Ribes meyeri Stem