Rapid separation and determination of spermine in fruit by capillary electrophoresis with electrochemiluminescence detection

Objective:To establish a capillary electrophoresis with electrochemiluminescence(CE-ECL)method for determination of spermidine in fruit. Methods:An efficient, rapid and accurate analytical approach was developed for the determination of spermine in fruit by CE-ECL technique. Results:The ECL intensity was linear with the concentrations of spermine over the ranges from 2×10^-4~3×10^-1 mmol/L. The linear equation was I=1114.2c+99.41, correlation coefficient r=0.9996(n=5)with detection limit(S/N=3)of 9.9×10^-5 mmol/L. The adding standard recoveries were 95.1%~101.2%, 99.0%~100.8% and 100.6%~106.0%, respectively. Conclusions:This method is simple, accurate, reproducible and can be used for the study of fruit fresh-keeping, and prolonging processing period.