Rapid separation and determination of spermine in fruit by capillary electrophoresis with electrochemiluminescence detection

WEI Ruo-nan; CHEN Zhi-yuan

doi:10.13386/j.issn1002-0306.2018.01.048

Volume 39 Issue 1

Dec. 2017

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Science and Technology of Food Industry > 2018 > 39(1): 267-271. > DOI: 10.13386/j.issn1002-0306.2018.01.048

WEI Ruo-nan, CHEN Zhi-yuan. Rapid separation and determination of spermine in fruit by capillary electrophoresis with electrochemiluminescence detection[J]. Science and Technology of Food Industry, 2018, 39(1): 267-271. DOI: 10.13386/j.issn1002-0306.2018.01.048

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Rapid separation and determination of spermine in fruit by capillary electrophoresis with electrochemiluminescence detection

WEI Ruo-nan¹,
CHEN Zhi-yuan^1,2, ,

1. College of Biological Science and Engineering, Shaanxi Sci-Tech University, Hanzhong 723001, China;
2. Bio-resources Key Laboratory of Shaanxi Province, Shaanxi Sci-Tech University, Hanzhong 723001, China

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Received Date: June 19, 2017
Available Online: December 28, 2020

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Abstract

Abstract

Objective:To establish a capillary electrophoresis with electrochemiluminescence(CE-ECL)method for determination of spermidine in fruit. Methods:An efficient, rapid and accurate analytical approach was developed for the determination of spermine in fruit by CE-ECL technique. Results:The ECL intensity was linear with the concentrations of spermine over the ranges from 2×10^-4~3×10^-1 mmol/L. The linear equation was I=1114.2c+99.41, correlation coefficient r=0.9996(n=5)with detection limit(S/N=3)of 9.9×10^-5 mmol/L. The adding standard recoveries were 95.1%~101.2%, 99.0%~100.8% and 100.6%~106.0%, respectively. Conclusions:This method is simple, accurate, reproducible and can be used for the study of fruit fresh-keeping, and prolonging processing period.
- capillary electrophoresis,
- electrochemiluminescence,
- Ru(pby)²⁺₃,
- spermine

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References

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Rapid separation and determination of spermine in fruit by capillary electrophoresis with electrochemiluminescence detection