Optimization of extraction conditions of intracellular polysaccharides from HL-003 using response surface methodology

ZHENG Peng-peng; LI Shan; CHEN Yu-hui; AO Xin-yu

doi:10.13386/j.issn1002-0306.2015.15.037

ZHENG Peng-peng, LI Shan, CHEN Yu-hui, AO Xin-yu. Optimization of extraction conditions of intracellular polysaccharides from HL-003 using response surface methodology[J]. Science and Technology of Food Industry, 2015, (15): 219-223. DOI: 10.13386/j.issn1002-0306.2015.15.037

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Optimization of extraction conditions of intracellular polysaccharides from HL-003 using response surface methodology

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Response surface methodology was used to optimize main process parameters for the extraction of polysaccharides from HL-003's intracellular broth based on polysaccharide extraction rate of one- factor tests.A regression model equation was fitted.The optimum extraction conditions were found to be ultrasound time 19 min,liquid- solid ratio 42 m L / g,extraction temperature 72 ℃ and extraction time 2.2 h.The extraction rate of polysaccharides under these conditions was up to 2.03%.The fitted regression model had excellent goodness of fit and showed lower prediction error.

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Optimization of extraction conditions of intracellular polysaccharides from HL-003 using response surface methodology

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