Optimization of hydrolysis process on solubility of protein from Antarctic krill(Euphausia superb)by response surface methodology

The solubility of the hydrolyzate of Antarctic krill protein was used as an index. Based on the single factor experiment,the effect of enzyme and substrate ratio,time,temperature and pH on the solubility of Antarctic krill protein hydrolyzate was analyzed by response surface optimization experiment. At the same time,the bast technology regression model of the solubility of the hydrolyzate and the factors was established and verified. The papain was screened from four enzymes to serve as proteolytic protease. On this basis,combining with the actual production situation,the optimal process conditions for the hydrolysis of Antarctic krill protein with ratio of papain to substrate:0.25%,hydrolysis time:30 min,hydrolysis temperature:55 ℃ and pH6.0 Under the optimal conditions,the solubility of Antarctic krill protein hydrolysates was(12.06%±0.21%). Therefore,enzymatic modification can change the solubility of Antarctic krill protein.