Optimization of Accelerated Solvent Extraction of Polysaccharides from <i>Gracilaria lemaneiformis</i> Using Response Surface Methodology and Anti-inflammatory Activity

WANG Huiying; LIU Yanfei; ZHANG Jingyuan; DU Bin; YANG Yuedong

doi:10.13386/j.issn1002-0306.2023030309

Volume 44 Issue 23

Nov. 2023

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Science and Technology of Food Industry > 2023 > 44(23): 110-117. > DOI: 10.13386/j.issn1002-0306.2023030309

WANG Huiying, LIU Yanfei, ZHANG Jingyuan, et al. Optimization of Accelerated Solvent Extraction of Polysaccharides from Gracilaria lemaneiformis Using Response Surface Methodology and Anti-inflammatory Activity[J]. Science and Technology of Food Industry, 2023, 44(23): 110−117. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023030309.

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Optimization of Accelerated Solvent Extraction of Polysaccharides from Gracilaria lemaneiformis Using Response Surface Methodology and Anti-inflammatory Activity

Hebei Key Laboratory of Natural Products Activity Components and Function, Qinhuangdao 066004, China

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Received Date: March 28, 2023
Available Online: October 08, 2023

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Abstract

The aim of this work was to apply response surface methodology (RSM) to model and optimize the accelerated solvent extraction (ASE) technique for extracting the crude polysaccharides (GLP-K) from Gracilaria lemaneiformis. In terms of the yield of polysaccharide, single factor tests and Box-Behnken design response surface method were developed. The structure of the prepared polysaccharides was characterized by Fourier transform infrared (FT-IR) and high performance liquid chromatography (HPLC). And the anti-inflammatory potential of GLP-K in lipopolysaccharide (LPS)-induced RAW264.7 macrophages was also explored. Briefly, the optimal extraction conditions for GLP-K were as follows: 70 °C of extraction temperature, 8.5 min of extraction time, and 4 extraction cycles. Under the condition, the experimental yield of polysaccharide was 9.58%±0.31%. FT-IR showed that the polysaccharide contained uronic acid, and the weight-average molecular weight ranged from 4.4 to 747.1 kDa. GLP-K had no significant cytotoxic effects at or below the concentrations of 1000 μg/mL (P<0.001). Compared with the model group, GLP-K administration group (50, 100, 200, 300, 400, 500 μg/mL) displayed remarkable inhibitory effects on the release level of NO (P<0.001), which decreased by 43.76%~69.47%. This paper enriched the research on the extraction of polysaccharides by accelerated solvent extraction technology, and provided experimental basis for the development and utilization of Gracilaria lemaneiformis polysaccharides.

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