Preparation of Peppermint Oil/<i>β</i>-CD Inclusion by High-speed Shearing with Process Optimization Using ResponseSurface Methodology

CHEN Hongbin; HONG Lunbo; WANG Juan; XU Jianbing; YANG Xiaoming; WU Xiaohui

doi:10.13386/j.issn1002-0306.2023020138

Volume 44 Issue 22

Nov. 2023

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Science and Technology of Food Industry > 2023 > 44(22): 210-218. > DOI: 10.13386/j.issn1002-0306.2023020138

CHEN Hongbin, HONG Lunbo, WANG Juan, et al. Preparation of Peppermint Oil/β-CD Inclusion by High-speed Shearing with Process Optimization Using ResponseSurface Methodology[J]. Science and Technology of Food Industry, 2023, 44(22): 210−218. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023020138.

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Preparation of Peppermint Oil/β-CD Inclusion by High-speed Shearing with Process Optimization Using ResponseSurface Methodology

1.
Zhejiang Huisong Pharmaceutical Company Limited, Hangzhou 310020, China
2.
College of Food and Biological Engineering, Jiangsu University, 212013, China

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Received Date: February 14, 2023
Available Online: September 16, 2023

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Abstract

Abstract

Objective: To investigate the optimal process for preparing peppermint essential oil/β-cyclodextrin (β-CD) inclusion complex using high-speed shearing combined with freeze-drying. Methods: The effects of freeze-drying and vacuum drying, high-speed shearing, and stirring on the encapsulation process were compared. Based on single-factor experiments, the ratio of β-CD to essential oil, encapsulation temperature, and mass fraction of β-CD were considered as influencing factors. The comprehensive score of oil content and encapsulation efficiency was used as an evaluation index to investigate the Box-Behnken response surface method for optimizing the encapsulation process. The content of pulegone in the inclusion complex was measured by high-performance liquid chromatography (HPLC). Fourier transform infrared spectroscopy (FTIR) was used to characterize the inclusion complex. Results: The yield of inclusion complex prepared by freeze-drying was higher, with an average yield of 97.6%. The oil content and encapsulation efficiency by the high-speed shearing method were relatively high, being about 3% and 19% higher than those by the stirring method, respectively. The optimized conditions were as follows: The weight ratio of β-CD to volatile oil was 9:1 (g/mL), the encapsulation temperature was 55 ℃, and the mass fraction of β-CD was 17%. Under these conditions, the essential oil content was 10.9%, the encapsulation efficiency was 97.6%, the comprehensive score was 99.7, the RSD value was 1.59%, and the optimized process was stable and feasible. The content of pulegone in the inclusion complex was found to be 2.465 mg/g by HPLC. The formation of the peppermint oil/β-CD inclusion complex was confirmed by analysis using FTIR. Conclusion: The combination of high-speed shearing and freeze-drying for the preparation of peppermint essential oil inclusion complex had the advantages of high efficiency, simple process, and it improved theyield, would provide guidance for the production process.
- peppermint essential oil,
- β-cyclodextrin inclusion,
- high-speed shearing,
- freeze-drying,
- Box-Behnken response surface,
- FTIR

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References

[1]	国家药典委员会. 中华人民共和国药典(第一部)[M]. 北京:中国医药科技出版社, 2020:394 National Pharmacopoeia Committee. Pharmacopoeia of the people's Republic of China (Part 1)[M]. Beijing:China Pharmaceutical Science and Technology Press, 2020:394.
[2]	艾米拉·奥肯, 吴涛, 白小慧, 等. GC-MS分析新疆4种薄荷挥发油化学成分及其生物活性的比较研究[J]. 食品研究与开发,2021,42(8):127−131 AOKEN A, WU T, BAI X H, et al. Comparative study of chemical composition and biological activity of essential oil from four species of mentha L. plants growing in Xinjiang by GC-MS[J]. Food Research and Develop,2021,42(8):127−131.
[3]	王霏娜, 俞箐, 找春霞, 等. 用响应面法优化 β-环糊精包合白术挥发油工艺[J]. 浙江大学学报,2008,42(12):2160−2165 WANG F N, YU J, ZHAO C X, et al. Optimization of preparation of Atractylodesmacrocephala volatile- β-cyclodextrin complex by response surface methodology[J]. Journal of Zhejiang University,2008,42(12):2160−2165.
[4]	CIOBANU A, MALLARD I, LANDY D, et al. Retention of aroma compounds from Menthapiperitaessential oil by cyclodextrins and crosslinkedcyclodextrinpolymers[J]. Food Chemistry,2013,138:291−297. doi: 10.1016/j.foodchem.2012.10.106
[5]	周瑶, 陈岗, 詹永, 等. 艾叶挥发油-环糊精包合物研究概述[J]. 食品工业,2020,41(8):240−243 ZHOU Y, CHEN G, ZHAN Y, et al. Summarization of study on folium Artemisiaargyi essential oil-cyclodextrin complexes[J]. Food Industry,2020,41(8):240−243.
[6]	徐蓉蓉, 高垚垚, 周云龙, 等. 薏苡仁油 β-环糊精包合物的制备及其表征[J]. 食品工业科技,2017,38(12):258−263 XU R R, GAO Y Y, ZHOU Y L, et al. Preparation and characterization of adlay seed oil and β-cyclodextrin inclusion complexes[J]. Science and Technology of Food Industry,2017,38(12):258−263.
[7]	马君义, 王宝生, 黄玉龙. 羟丙基- β-环糊精对百里香挥发油包合作用的研究[J]. 食品工业科技,2013,31(9):259−260 MA J Y, WANG B S, HUANG Y L. Inclusion complex of volatile oil from Thymus mongolicusronn. with hydroxypropyl- β-cyclodextrin[J]. Science and Technology of Food Industry,2013,31(9):259−260.
[8]	陆宁, 宛晓春, 刘文琴. 薄荷油 β-环糊精包合效果的多指标综合评判[J]. 粮油食品科技,2004,12(2):23−24 doi: 10.3969/j.issn.1007-7561.2004.02.010 LU N, WAN X C, LIU W Q. The comprehensive evaluate of inclusion effect about peppermint oil and β-cyclodextrin with multiple-factor[J]. Science and Technology of Cereals, Oils and Foods,2004,12(2):23−24. doi: 10.3969/j.issn.1007-7561.2004.02.010
[9]	XI X H, HUANG J L, ZHANG S Y, et al. Preparation and characterization of inclusion complex of Myristicafragrans Houtt (nutmeg) essential oil with 2-hydroxypropyl-β-cyclodextrin[J]. Food Chemistry, 2023, 423(15): 136316.
[10]	陈秋实, 张洪, 吴杰. 薄荷挥发油的提取及其 β-环糊精包合物制备工艺研究[J]. 云南中医学院学报,2008,31(1):13−16 CHEN Q S, ZHANG H, WU J, et al. Extraction of volatile oil from Menthehapiocalyx Briq. and processing techniques of it’s β-cycoldextrin in clusion compound[J]. Journal of Yunnan University of Traditional Chinese Medicine,2008,31(1):13−16.
[11]	JIANG S, ZHAO T T, WEI Y Y, et al. Preparation and characterization of tea tree oil/hydroxypropyl- β-cyclodextrin inclusion complex and its application tocontrol brown rot in peach fruit[J]. Food Hydrocolloids,2021,121(1):107037.
[12]	石春韬, 李萍, 舒婷, 等. 肉桂油- β-环糊精纳米微胶囊的超声波法制备与表征[J]. 食品工业科技,2016,37(18):307−323 SHI C T, LI P, SHU T, et al. Ultrasonic preparation and characterization of cinnamon oil- β-cyclodextrin nanocapsules[J]. Science and Technology of Food Industry,2016,37(18):307−323.
[13]	王鸿森, 徐彦浩, 常晟, 等. 应用高压均质技术制备白术挥发油- β-环糊精包合物[J]. 泰山医学院学报,2017,38(6):607−609 WANG H S, XU Y H, CHANG S, et al. Fabrication of baizhu’s volatile oil- β-cyclodextrin inclusion complex by high pressure homogenization method[J]. Journal of Taishan Medical College,2017,38(6):607−609.
[14]	李双双, 李希, 邓谦, 等. 椒香温中止痛方挥发油 β-环糊精包合工艺优化及其包合物表征[J]. 中成药,2020,42(12):3129−3134 LI S S, LI X, DENG Q, et al. Optimization of β-cyclodextrin inclusion process for volatile oils in jiaoxiang wenzhong zhitong decoction and characterization of inclusion compounds[J]. Chinese Traditional Patent Medicine,2020,42(12):3129−3134.
[15]	国家药典委员会. 中华人民共和国药典(第四部)[M]. 第四版. 北京:中国医药科技出版社, 2020:233 National Pharmacopoeia Committee. Pharmacopoeia of the people's Republic of China (Part 4)[M]. Beijing:China Pharmaceutical Science and Technology Press, 2020:233.
[16]	屈云萍, 李哲, 王娇, 等. 舒筋通络颗粒中乳香挥发油提取及包合工艺研究[J]. 河北工业科技,2018,35(4):294−298 QU Y P, LI Z, WANG J, et al. Study on extraction and inclusion process of volatile oil from frankincense in Shujintongluo granules[J]. Hebei Journal of Industrial Scienceand Technology,2018,35(4):294−298.
[17]	高杰, 李哲, 李雪利, 等. Box-Behnken响应面法优化徐长卿配方颗粒挥发油- β-CD包合物制备工艺[J]. 河北工艺科技,2018,35(5):377−382 GAO J, LI Z, LI X L, et al. Optimization of the preparation of volatile oil from cynanchumpaniculatum formula granule- β-cyclodextrin inclusion complex by Box-Behnken design-response surface method[J]. Hebei: Journal of Industrial Science and Technology,2018,35(5):377−382.
[18]	RYZHAKOV A, DO T T, STAPPAERTS J, et al. Serf-assembly of cyclodextrins and their complexes in aqueous solutions[J]. Journal of Pharmaceutical Sciences,2016,105(9):2556−2569. doi: 10.1016/j.xphs.2016.01.019
[19]	LIU Y Y, ZHANG G S, ZHU G W, eta1. Using the Box-Behnken response surface method to optimize the preparation and characterization of lavender and fennel volatile oil β-cyclodextrin form inclusion complex[J]. World Journal of Traditional Chinese Medicine,2018,4(2):54−61. doi: 10.4103/wjtcm.wjtcm_9_18
[20]	张志军, 张桂芝, 王朋朋. 广藿香挥发油的红外光谱鉴定和气相色谱-质谱分析[J]. 中国医药科学,2015,5(1):86−91 ZHANG Z J, ZHANG G Z, WANG P P. Analysis of essential oils of Pogostemonisherba by FTIR and GC-MS[J]. China Medicine and Pharmacy,2015,5(1):86−91.
[21]	王媚, 吴建华, 史亚军, 等. 红花挥发油的红外光谱鉴定与气相色谱-质谱分析[J]. 化学与生物工程,2017,34(3):66−70 WANG M, WU J H, SHI Y J, et al. Analysis of volatile oil from Carthamustinctorius L. by infrared spectroscopy and gas chromatography-mass spectrometry[J]. Chemistry and Bioengineering,2017,34(3):66−70.
[22]	孙兰萍, 马龙, 伍亚华, 等. 杜仲籽油 β-环糊精包合物的制备及其表征[J]. 食品工业科技,2013,34(6):315−319 SUN L P, MA L, WU Y H, et al. Preparation and characterization of β-cyclodextrin inclusion complex of Eucommiaulmoides seed oil[J]. Science and Technology of Food Industry,2013,34(6):315−319.
[23]	李雪利, 高杰, 李哲, 等. 响应面法优化香橼配方颗粒挥发油包合工艺[J]. 现代医药卫生,2018,34(22):3479−3482 LI X L, GAO J, LI Z, et al. Response surface method for optimizing volatile oil inclusion technology of citron formula granules[J]. Journal of Modern Medicine and Health,2018,34(22):3479−3482.
[24]	金哲雄, 王玥, 周群, 等. 闹羊花与野菊花红外光谱的分析与鉴定[J]. 2014, 34(9):2434-2438 JIN Z X, WANG Y, ZHOU Q, et al. The analyses and identification of Flosrhododendrimollis and Floschrysanthemiindici viainfrared spectroscopy[J]. Spectroscopy and Spectral Analysis, 2014, 34(9):2434-2438.
[25]	ALIZADEH N, NAZARI F. Thymol essential oil/ β-cyclodextrin inclusion complex into chitosan nanoparticles:Improvement of thymol properties in vitro studies[J]. Journal of Molecular Liquids,2022,346:118250. doi: 10.1016/j.molliq.2021.118250
[26]	AMBROZEJ K K, MARTYNA A, KARPINSKA J, et al. Quality control of mint species based on UV-VIS and FTIR spectral data supported by chemometric tools[J]. Food Control,2021,129:108228. doi: 10.1016/j.foodcont.2021.108228
[27]	潘铁英, 张玉兰, 苏克曼. 波谱解析法[M]. 第二版. 华东理工大学出版社, 2010:110-113 PAN T Y, ZHANG Y L, SU K M. Wave spectrum analysis method[M]. Second edition. East China University of Science and Technology Press, 2010:110-113.
[28]	HADIAN Z, KAMALABADI M, PHIMOLSIRIPOL Y, et al. Preparation, characterization, and antioxidant activity of β-cyclodextrin nanoparticles loaded Rosa damascena essential oil for application in beverage[J]. Food Chemistry,2023,403:134410. doi: 10.1016/j.foodchem.2022.134410
[29]	LUO X M, ZENG L X, LI Q, et al. β-Cyclodextrin inclusion complex containing essential oil from wampee [ Clausena lansium (Lour.) Skeels] fruit pericarp:Synthesis, characterization, and evaluation of antioxidant activity[J]. Journal of Molecular Structure,2022,1266:133525. doi: 10.1016/j.molstruc.2022.133525
[30]	EMADZADEH B, GHORANI B, TABASI S N, et al. Fate of β-cyclodextrin-sugar beet pectin microcapsules containing garlic essential oil in an acidic food beverage[J]. Food Bioscience,2021,42:101029. doi: 10.1016/j.fbio.2021.101029
[31]	李娜, 刘德旺, 赵红梅, 等. 四味土木香散挥发油的 β-环糊精包合工艺[J]. 中成药,2020,42(3):558−563 LI N, LIU D W, ZHAO H M, et al. β-Cyclodextrin inclusion process for volatile oils of siweitumuxiang powder[J]. Chinese Traditional Patent Medicine,2020,42(3):558−563.

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