One-step Preparation and Characterization of Water-dispersible Astaxanthin Emulsion by Tween-20 under Ultrasonic Condition

LI Jia; QI Xiangming

doi:10.13386/j.issn1002-0306.2023020089

Volume 44 Issue 22

Nov. 2023

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Science and Technology of Food Industry > 2023 > 44(22): 182-190. > DOI: 10.13386/j.issn1002-0306.2023020089

LI Jia, QI Xiangming. One-step Preparation and Characterization of Water-dispersible Astaxanthin Emulsion by Tween-20 under Ultrasonic Condition[J]. Science and Technology of Food Industry, 2023, 44(22): 182−190. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023020089.

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One-step Preparation and Characterization of Water-dispersible Astaxanthin Emulsion by Tween-20 under Ultrasonic Condition

LI Jia^{1, 2, 3,},
QI Xiangming^{1, 2, 3, ,}

1.
Qingdao Key Laboratory of Food Biotechnology, College of Food Science and Engineering,Ocean University of China, Qingdao 266404, China
2.
Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory forMarine Science and Technology, Qingdao 266237, China
3.
Key Laboratory of Biological Processing of Aquatic Products, China National Light Industry, Qingdao 266404, China

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Received Date: February 09, 2023
Available Online: September 18, 2023

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Abstract

Abstract

In order to achieve sustainable extraction and enhance the properties of astaxanthin from Haematococcus pluvialis, a green and efficient approach was developed by utilizing Tween-20 under ultrasonic conditions. This approach allowed for the efficient release of astaxanthin while simultaneously preparing water-dispersible astaxanthin emulsions. The factors influencing the process, the formation mechanism of the water-dispersible astaxanthin emulsion, and the composition and performance characterization of the obtained product were investigated. Exploration of the process revealed that Tween-20 dosage, ultrasonic power, and solid-to-liquid ratio significantly influenced the release and dispersion efficiency of astaxanthin, with 98.41% astaxanthin being released and dispersed when Tween-20 dosage, ultrasonic power, and solid-to-liquid ratio were 200 μL, 600 W, and 1:20 g/mL, respectively. The mechanism exploration results demonstrated that ultrasound had a dual effect of disrupting the cells of H. pluvialis to a limited extent and assisting Tween-20 to disperse astaxanthin, which was superior to high-pressure homogenization. The characterization results showed that the water-dispersible astaxanthin emulsion obtained under these conditions had an average particle size of 115.55 nm, a Zeta potential of −23.35 mV, and an encapsulated astaxanthin content of up to 43.82% in the dry product. Astaxanthin was found to be encapsulated in an amorphous non-crystalline state, with selective encapsulation of saturated fatty acids. The amount of saturated fatty acids was up to 17.93% (wt.%). Overall, this one-step, ultrasonic approach to preparing water-dispersible astaxanthin emulsions from H. pluvialis using Tween-20 is easy to operate, highly efficient, green and feasible, with some of the product properties being superior to those of pure astaxanthin encapsulated products.
- astaxanthin,
- ultrasound,
- release,
- Tween-20,
- nanoemulsion,
- component analysis,
- performance characterization

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References (38)

References

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